A5010479759- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Biomedical and Engineering Education
- Silk-based biomaterials and applications
- RNA regulation and disease
- Corneal Surgery and Treatments
- Innovations in Medical Education
- Pluripotent Stem Cells Research
- Biochemical and Structural Characterization
- Electrospun Nanofibers in Biomedical Applications
- Vagus Nerve Stimulation Research
- Pleural and Pulmonary Diseases
- Nerve injury and regeneration
- Mobile Learning in Education
- EEG and Brain-Computer Interfaces
- Augmented Reality Applications
- Virus-based gene therapy research
- Simulation-Based Education in Healthcare
AVEO Oncology (United States)
2025
Sanofi (United States)
2025
Biogen (United States)
2020-2021
Northeastern University
2016-2020
Tufts University
2015-2017
We present a silk biomaterial platform with highly tunable mechanical and degradation properties for engineering regeneration of soft tissues such as, skin, adipose, neural tissue, elasticity in the kilopascal range. Lyophilized sponges were prepared under different process conditions effect molecular weight, concentration crystallinity on 3D scaffold formation, structural integrity, morphology, properties, cell interactions vitro vivo studied. Tuning weight distribution (via degumming time)...
Abstract CRISPR-Cas systems have emerged as a powerful tool to generate genetic models for studying normal and diseased central nervous system (CNS). Targeted gene disruption at specific loci has been demonstrated successfully in non-dividing neurons. Despite its simplicity, high specificity low cost, the efficiency of CRISPR-mediated knockout vivo can be substantially impacted by many parameters. Here, we used CRISPR-Cas9 disrupt neuronal-specific gene, NeuN, optimized key parameters...
With insufficient options to meet the clinical demand for cornea transplants, one emerging area of emphasis is on tissue engineering. In present study, goal was combine corneal stroma and epithelium into coculture system, monitor both human stromal stem cell (hCSSC) epithelial (hCE) growth differentiation keratocytes differentiated in these three-dimensional systems vitro. Coculture conditions were first optimized, including medium, air–liquid interface culture, surface topography chemistry...
Adeno-associated virus (AAV)-based therapeutics have the potential to transform lives of patients by delivering one-time treatments for a variety diseases. However, critical challenge their widespread adoption and distribution is high cost goods. Reducing manufacturing costs developing AAV capsids with improved yield, or fitness, key making gene therapies more affordable. fitness largely determined amino acid sequence capsid, however, engineered AAVs are rarely optimized manufacturability....
Adeno-associated virus (AAV) transduction efficiency and tropism are conventionally determined by high expression of a fluorescent reporter gene. Emerging data has suggested that such conventional methods may underestimate AAV for cells in which from vectors is undetectable. To explore an alternative method captures low cargo sufficient the intended activity, we sought after CRISPR/Cas9-mediated gene disruption. In this study, use to deliver CRISPR/guide RNA designed abolish genes NeuN,...
Abstract Tissue‐engineered models continue to experience challenges in delivering structural specificity, nutrient delivery, and heterogenous cellular components, especially for organ‐systems that require functional inputs/outputs have high metabolic requirements, such as the heart. While soft lithography has provided a means recapitulate complex architectures dish, it is plagued with number of prohibitive shortcomings. Here, concepts from microfluidics, tissue engineering, layer‐by‐layer...
Medical manikins are a type of patient simulator used to train medical staff. The benefits using manikin include the suspension disbelief, simultaneous team and individual learning, allowable failure, personalized scenarios, frequent repetition focus on needs learner rather than patient. Unfortunately, one limitations is that they cannot simulate every problem staff comes across. SimMan (Laerdal) do not have ability when excess air or fluid in pleural space lung thorax, drainage which must...
Cardiac sympathovagal imbalance is a dysfunction of the parasympathetic and sympathetic nervous systems (PSNS SNS) that, in tandem, regulate cardiac output response to environmental stimuli. This autonomic system (ANS) increases risk arrhythmias sudden failure due an inability modulate heart rate following overexertion or excessive stress. Therefore, there immediate need understand underlying cellular mechanisms innervation develop new strategies restore ANS balance. Moving away from...
One hot July morning, I put on a business casual dress and headed to biotech company for the first day of my internship. It felt daunting. Academia was safe space; had never worked anywhere else. When arrived at office, ushered into an orientation room, where people in formal attire told me about company's mission, products, research areas. As morning wore on, unease working for-profit grew. left wondering whether pausing Ph.D. take internship been mistake. > “I'd spent years dreaming up...
To overcome the limitations of traditional organ chips, in article number 2000133, Ryan A. Koppes and co-workers have implemented a “cut assemble” manufacturing technique to exploit meniscus pinning effect, via GelPins, constrain 3D biomaterials. These GelPins enable formation discrete, yet contiguous hydrogel structures both x-y z planes within custom microfluidic devices, facilitating future investigation innervation heterogenous tissue interactions.