A5021021395- Protein Degradation and Inhibitors
- ATP Synthase and ATPases Research
- interferon and immune responses
- Epigenetics and DNA Methylation
- Heavy Metal Exposure and Toxicity
- Neuroblastoma Research and Treatments
- Computational Drug Discovery Methods
- Birth, Development, and Health
- Cancer therapeutics and mechanisms
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Virus-based gene therapy research
- Chromosomal and Genetic Variations
- Effects and risks of endocrine disrupting chemicals
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Nanoparticles: synthesis and applications
- Mosquito-borne diseases and control
- IoT-based Smart Home Systems
- RNA Research and Splicing
University of Alabama at Birmingham
2024
Salk Institute for Biological Studies
2019-2023
Wayne State University
2012-2017
Detroit R&D (United States)
2015
Environmental Health
2015
This study explores the use of human embryonic stem cells (hESCs) for assessing nanotoxicology, specifically, effect gold nanoparticles (AuNPs) different core sizes (1.5, 4, and 14 nm) on viability, pluripotency, neuronal differentiation, DNA methylation hESCs. The hESCs exposed to 1.5 nm thiolate‐capped AuNPs exhibit loss cohesiveness detachment suggesting ongoing cell death at concentrations as low 0.1 μg mL −1 . this concentration do not form embryoid bodies but rather disintegrate into...
Abstract We report that the DNA methylation profile of a child’s neonatal whole blood can be significantly influenced by his or her mother’s lead levels (BLL). recruited 35 mother-infant pairs in Detroit and measured (Pb) at over 450,000 loci from current both mother child. found mothers with high BLL correlate altered 564 their children’s blood. Our results suggest Pb exposure during pregnancy affects status fetal germ cells, which leads to grandchildren’s dried spots. This is first...
Exposure to lead (Pb) during childhood can result in learning disabilities and behavioral problems. Although described animal models, whether Pb exposure also alters neuronal differentiation the developing brains of exposed children is unknown. Here, we investigated effects physiologically relevant concentrations (from 0.4 1.9μM) on capacity human embryonic stem cells (hESCs) progress a fate. We found that neither acute nor chronic prevented hESCs from generating neural progenitor (NPCs)....
Aims: In this paper, we tested the hypothesis that early life lead (Pb) exposure associated DNA methylation (5 mC) changes are dependent on sex of child and can serve as biomarkers for Pb exposure. Methods: pilot study, measured 5mC profiles extracted from dried blood spots (DBS) in a cohort 43 children (25 males 18 females; ages 3 months to 5 years) Detroit. Result & Discussion: We found effect Pb-exposure 5-mC be separated into three subtypes: affected loci which conserved irrespective...
Prenatal exposure to neurotoxicants such as lead (Pb) may cause stable changes in the DNA methylation (5mC) profile of fetal genome. However, few studies have examined its effect on de-methylation pathway, specifically dynamic 5-hydroxymethylcytosine (5hmC) profile. Therefore, this study, we investigate relationship between Pb and 5mC 5hmC modifications during early development. To study profile, use a novel modification Infinium™ HumanMethylation450 assay (Illumina, Inc.), which named...
Traumatic brain injury (TBI) can cause persistent pathological alteration of neurons. This may lead to cognitive dysfunction, depression and increased susceptibility life threatening diseases, such as epilepsy Alzheimer's disease. To investigate the underlying genetic molecular basis TBI, we subjected w1118 Drosophila melanogaster mild closed head trauma found that mitochondrial activity is reduced in brains these flies 24 hours after inflicting trauma. determine transcriptomic changes...
GATA3 is essential for T cell differentiation and surrounded by genome-wide association study (GWAS) hits immune traits. Interpretation of these GWAS challenging because gene expression quantitative trait locus (eQTL) studies lack power to detect variants with small effects on in specific types the genome region containing contains dozens potential regulatory sequences. To map sequences GATA3, we performed a high-throughput tiling deletion screen 2 Mb Jurkat cells. This revealed 23 candidate...
Abstract Genetic variants and de novo mutations in regulatory regions of the genome are typically discovered by whole-genome sequencing (WGS), however WGS is expensive most reads come from non-regulatory regions. The Assay for Transposase-Accessible Chromatin (ATAC-seq) generates sequences could potentially be used as a low-cost ‘capture’ method variant discovery, but its use this purpose has not been systematically evaluated. Here we apply seven callers to bulk single-cell ATAC-seq data...
Abstract Glioblastoma is the most prevalent primary malignant brain tumor in adults and characterized by poor prognosis universal recurrence. Effective glioblastoma treatments are lacking, part due to somatic mutations epigenetic reprogramming that alter gene expression confer drug resistance. To investigate recurrently dysregulated genes glioblastoma, we interrogated allele-specific (ASE), difference between two alleles of a gene, stem cells (GSC) derived from 43 patients. A total 118 were...
Abstract Background Neuroblastoma is a pediatric malignancy with high frequency of metastatic disease at initial diagnosis. tumors have few recurrent protein-coding mutations but contain extensive somatic copy number alterations (SCNAs) suggesting that alter gene dosage are important drivers tumorigenesis. Here, we analyze allele-specific expression in 96 high-risk neuroblastoma to discover genes impacted by cis-acting dosage. Results We identify 1043 recurrent, neuroblastoma-specific...
The SNF2 family chromatin remodeler HELLS has emerged as an important regulator of cell proliferation, genome stability, and several cancer pathways. Significant upregulation been reported in 33 human types. While implicated DNA damage response, its function repair is poorly understood. Here we report a new regulatory link between single-strand break (SSB) cellular responses to alkylation damage. We found that loss impairs SSB repair, selectively sensitizes cells alkylating agents PARP...
Abstract B-cell acute lymphoblastic leukemia (B-ALL) is the most common pediatric malignancy. Based on gene expression profiling, B-ALL can be classified into distinct transcriptional subtypes with differing disease outcomes. Many of these are defined by mutations in transcription factors and chromatin-modifying enzymes, but how such diverse lead to remains unclear. To illuminate chromatin regulatory landscape B-ALL, we analyzed 3D genome organization, open chromatin, 53 primary patient...
Abstract Traumatic brain injury (TBI) can cause persistent pathological alteration of neurons. This may lead to cognitive dysfunctions, depression, and even increased susceptibility life threatening diseases, such as epilepsy Alzheimer’s Disease. To investigate the underlying genetic molecular basis TBI, Wasserman colleagues developed an inexpensive reproducible model for simulating TBI in Drosophila melanogaster (Fruit Fly). Using a modified version this high impact trauma (HIT) device, we...
Abstract: The basic idea of our project is to make a Monitoring and Alert System using Embedded Systems IOT. Our deals with various kinds sensors (like temperature sensors, gas etc.) other peripherals along an Arduino UNO Microcontroller Wi-Fi module. Here all these will collect data from the surrounding, send it which further website also alert if any sort unwanted conditions observed. sensor remotely help via Internet Things main purpose project. We believe that this provide users simple,...
<div>Abstract<p>Glioblastoma is the most prevalent primary malignant brain tumor in adults and characterized by poor prognosis universal recurrence. Effective glioblastoma treatments are lacking, part due to somatic mutations epigenetic reprogramming that alter gene expression confer drug resistance. To investigate recurrently dysregulated genes glioblastoma, we interrogated allele-specific (ASE), difference between two alleles of a gene, stem cells (GSC) derived from 43...
Supplementary Data from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma
Supplementary Data from Leveraging Allele-Specific Expression for Therapeutic Response Gene Discovery in Glioblastoma