A5084976435- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Growth Hormone and Insulin-like Growth Factors
- Ovarian function and disorders
- Endometriosis Research and Treatment
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and disorders
- Uterine Myomas and Treatments
- Family Dynamics and Relationships
- Genetic Syndromes and Imprinting
- Pancreatic function and diabetes
- Sperm and Testicular Function
- Ovarian cancer diagnosis and treatment
- Thyroid Disorders and Treatments
- Neonatal Respiratory Health Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Birth, Development, and Health
- Cancer, Hypoxia, and Metabolism
- Folate and B Vitamins Research
- COVID-19 Impact on Reproduction
- Gynecological conditions and treatments
Väestöliitto
2006-2019
Aava Medical Centre
2019
Turku University Hospital
1999
Monash University
1995-1998
Helsinki University Hospital
1987-1996
Monash Medical Centre
1995
Epworth Hospital
1995
Monash IVF
1995
Royal Prince Alfred Hospital
1991-1993
University of Helsinki
1988-1991
The serum levels of 34K insulin-like growth factor (IGF)-binding protein were measured by RIA in 88 type 1 diabetic patients, 9 patients with 2 diabetes, 7 insulinoma, 19 normal subjects (all after an overnight fast), and 82 a breakfast meal. In addition, the effect 2- to 3-h euglycemic steady state hyperinsulinemia on IGF-binding IGF-1 was studied some each fasted groups. Compared subjects, mean 4-fold (P less than 0.001) higher I treated conventional insulin injections, 2.5-fold those...
The placenta expresses genes for insulin-like growth factors (IGFs) and possesses IGF-receptors, suggesting that placental is regulated by IGFs in an autocrine manner. We have previously shown human decidua, but not placenta, synthesizes secretes a 34 K IGF-binding protein (34 IGF-BP) called 12. now used choriocarcinoma JEG-3 cell monolayer cultures recombinant (Thr59)IGF-I as model to study whether the decidual IGF-BP able modulate receptor binding biological activity of trophoblasts....
The number of children born after frozen embryo transfer (FET) is steadily rising. However, studies on obstetric and perinatal outcomes are limited. Our primary aim was to compare the health FET fresh transfer, use data from spontaneous conception as a reference. In register-based cohort study we evaluated (n = 2293), 4151) those pregnancy (reference group; n 31 946). Data were collected registers two infertility outpatient clinics, university hospitals Finnish Medical Birth Register...
Journal Article Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection assisted hatching Get access Frank L. Barnes, Barnes Search for other works by this author on: Oxford Academic PubMed Google Scholar Amanda Crombie, Crombie 2Monash IVF Pty Ltd, Epworth HospitalErin Street, Richmond, Victoria 3121, Australia David K. Gardner, Gardner Annette Kausche, Kausche Orly Lacham-Kaplan, Lacham-Kaplan Anne-Maria Suikkari, Suikkari...
We previously demonstrated that supraphysiological insulin concentrations reducedthe plasma 34K insulinlike growth factor-binding protein (IGF-BP) in humans. Inthis study we examined whether physiological changes regulateIGF-BP and, if so, the regulation is influenced by race, glucose tolerance, or rate of metabolism. For these purposes 1) analyzed relationship between fasting and IGF-BP 2 racial groups (23 Caucasians 35 southwestern American Indians), 2) measured response to oral 20 normal...
BACKGROUND: The purpose has been to develop an in vitro oocyte maturation (IVM) method for a wide range of patients. METHODS: A total 239 cycles with immature retrieval (IOC) were carried out without hormonal priming. Patients regular and normal or polycystic ovaries (PCO) anovulatory PCOS included. Insemination intracytoplasmic sperm injection (ICSI) according quality was alternatively used fertilization the matured oocytes. RESULTS: 971 oocytes (mean 8.0±5.2) collected 122 IVM-IVF 851...
Careful follow-up of children born after in vitro maturation (IVM) human oocytes is essential because the technique still very new.Obstetric and perinatal data were collected from all deliveries IVM treatment during 1999-2004. The growth development was assessed at 6, 12 24 months using Muenchener Funktionelle Entwicklungs Diagnostik Bayley Scales Infants.In total, 43 women [age 31.2 +/- 3.9 (mean SD) years] gave birth to 40 singleton infants three sets twins (multiple rate 7.0%). Obstetric...
Insulin-like growth factors IGF-I and IGF-II are bound to specific binding proteins in serum. The lower mol. wt protein (IGF-BP) has been detected various tissues, including secretory endometrium preovulatory follicles of the ovary. This group studied circulating levels IGF-BP serum 23 patients with polycystic ovarian disease (PCOD) found that one-third them have a subnormal level. In comparison PCOD normal level, those level had higher degree obesity tendency be more hirsute. They also...
A β-lactoglobulin homologue (βLG/PP14) and insulin-like growth factor-binding protein-1 (IGFBP-1) are two major secretory proteins of the human endometrium. In present study, we have shown that βLG/PP14 mRNA is expressed in endometrium a cyclic manner, being hardly detectable midcycle most abundant during late phase. IGFBP-1 also endometrium, but amounts smaller than those encoding with maximal accumulation earlier The expression these mRNAs occurs different cell types as revealed by situ...
We studied the response of serum 34K insulinlike growth factor-binding protein (IGF-BP) to two types prolonged exercise. In first study, 11 normal men performed cycle ergometer exercise, after an overnight fast, for 3 h at intensity corresponding 45–50% their maximal aerobic power. After mean IGF-BP concentration was 4.9-fold higher than baseline level (P < 0.01), while IGF-I did not change. Resting same time period resulted in a 38% fall 0.01). second 10 and 8 type 1 diabetic exercised, on...
Does the length of time during which embryos are cultured in vitro affect birthweight infants?The duration embryo culture period is a significant factor determining infants.IVF children show adverse perinatal outcome when compared with general population and increased incidence preterm birth low commonly observed.A retrospective cross-sectional cohort study including 1079 infants born after treatment at Family Federation Finland Fertility Clinic Helsinki, between 2000 2010.All singleton IVF...
BACKGROUND: Recent studies have shown that zygote morphology could be used for the assessment of human embryo quality. Pronuclear (PN) is based on certain distinct features seen in zygotes 16–18 h after fertilization. In present study PN stage was assessed and combined with a single transfer order to investigate whether currently classifications are able predict quality implantation rates. METHODS AND RESULTS: Zygotes were analysed according two different classification systems. first, total...
Human follicular fluid contains insulin-like growth factor I (IGF-I) and its low molecular weight binding protein (IGF-BP). We studied the synthesis of IGF-BP by granulosa cells obtained after ovarian hyperstimulation for in vitro fertilization. The were cultured 72 hours Ham's F-10 medium supplemented with 10 % fetal calf serum (FCS). Samples culture collected every 24 hours. concentration increased from 1.2 to 2.1 μg/1 at 48 h 3.3 h. De novo was shown incorporation labeled methionine into...
Frozen embryo transfers are characterized by impaired pregnancy outcome and increased incidence of loss as compared with fresh IVF/ICSI transfers. In this study, we performed a retrospective analysis clinical embryological factors that potentially influence the frozen transfer.We reviewed 1242 respect to age woman, method fertilization, quality before after freezing number embryos transferred.The (positive hCG) rates were 25.8 21.1%, respectively. A total 107 (33.3%) 321 pregnancies...
Worldwide there is an increasing number of families created by oocyte donation (OD). The aim this study was to gather information about parents' plans disclosure their child and other people, as well attitudes level satisfaction up 15 years after OD treatment. A questionnaire with separate material for each partner sent all parents (167 mothers, 163 fathers) who had a treatment donated oocytes at Väestöliitto Fertility Clinics in Helsinki during 1992–2006. These total 231 children aged 1–14...
In this prospective study we investigated whether the maturation and fertilization of immature oocytes can be improved by administration recombinant follicle stimulating hormone (rFSH) starting in late luteal phase two groups women: group 1 (n = 6) women with regular menstrual cycles; 2 irregular cycles polycystic ovaries (PCO) on ultrasound examination. Low-dose (37.5 IU) rFSH was commenced 11 days after LH surge during a spontaneous cycle ninth day progesterone an cycle. Recombinant FSH...
Is an elective single-embryo transfer (eSET) policy feasible for women aged 40 or older?For older (aged 40-44 years) with a good prognosis, eSET can be applied acceptable cumulative clinical pregnancy rates and live birth rates.Various studies have shown the effectiveness of in <35 years high low multiple births.This retrospective cohort study included 628 treated between 2000 2009.Women underwent fresh cycle IVF ICSI treatment (n = 264) double-embryo (DET) 364). In subsequent frozen-thawed...
What are the short- and long-term health consequences current satisfaction of altruistic oocyte donors?Donating oocytes in majority (>90%) donation cycles is not associated with harmful general or reproductive effects.Knowledge effects on donors sparse follow-up studies have usually been carried out commercial donors. Thus far, no major demonstrated. Most reported a high level donor satisfaction, but also less favorable experiences published.A retrospective cross-sectional survey all women...
Insulin-like growth factor-binding protein-3 (IGFBP-3) carries most of the serum IGFs as a 150K ternary complex which increases during pregnancy. However, recent studies have demonstrated that IGFBP-3 is absent in pregnancy when measured by ligand blotting after electrophoresis. In present study we demonstrate that, several criteria, appears functionally intact serum. Serum samples were collected from pregnant women between 2-40 weeks gestation. immunoreactive and acid-labile (alpha) subunit...
In order to study the effects of insulin-like growth factor (IGF-I) and binding protein (IGFBP-1) on human granulosa cell proliferation after in vitro fertilization, cells were obtained oocyte retrieval cultured presence or absence graded amounts recombinant IGF-I, purified IGFBP-1 [3H] thymidine Physiological concentrations IGF-I (2–200 ng/ml) found stimulate incorporation into a concentration-dependent manner. Half-maximal stimulation was with 10 ng/ml exogenous which chosen for...
What are disclosure intentions and experiences of heterosexual parents with children born after assisted donor insemination (DI) or IVF sperm (dIVF)? Only 16.5% Finnish DI/dIVF had told their child his/her origin; 18% all above 3 years age received the information. Parents older were more unwilling to tell uncertain regarding what do than younger children. In general, 10–35% DI offspring willing inform about conception. Men who need become a father secretive women donated oocytes less...