A5022307929- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Microbial metabolism and enzyme function
- Peptidase Inhibition and Analysis
- Fluid Dynamics Simulations and Interactions
- Microbial Fuel Cells and Bioremediation
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Microbial Metabolic Engineering and Bioproduction
University of Michigan
2022-2024
Pohang University of Science and Technology
2021
Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration colocalization can increase catalytic activity, stability, longevity, highly desirable features for many biotechnological biomedical applications enzyme catalysts. One promising strategy to construct is repurpose protein nanocages found in nature. However, protein-based often exhibit decreased partially caused by mismatch shell selectivity the substrate...
Protein nanocages have emerged as an important engineering platform for biotechnological and biomedical applications. Among naturally occurring protein cages, encapsulin nanocompartments recently gained prominence due to their favorable physico-chemical properties, ease of shell modification, highly efficient selective intrinsic packaging capabilities. Here, we expand function by designing characterizing encapsulins concurrent RNA encapsulation in vivo. Our strategy is based on modifying...
Abstract Enzyme nanoreactors are nanoscale compartments consisting of encapsulated enzymes and a selectively permeable barrier. Sequestration co-localization can increase catalytic activity, stability, longevity, highly desirable features for many biotechnological biomedical applications enzyme catalysts. One promising strategy to construct is repurpose protein nanocages found in nature. However, protein-based often exhibit decreased partially caused by mismatch shell selectivity the...
Encapsulins are self-assembling protein nanocompartments able to selectively encapsulate dedicated cargo enzymes. widespread across bacterial and archaeal phyla involved in oxidative stress resistance, iron storage, sulfur metabolism. Encapsulin shells exhibit icosahedral geometry consist of 60, 180, or 240 identical subunits. Cargo encapsulation is mediated by the specific interaction targeting peptides domains, found all proteins, with interior surface encapsulin shell during...
Abstract Protein nanocages have emerged as an important engineering platform for biotechnological and biomedical applications. Among naturally occurring protein cages, encapsulin nanocompartments recently gained prominence due to their favorable physico-chemical properties, ease of shell modification, highly efficient selective intrinsic packaging capabilities. Here, we expand function by designing characterizing encapsulins concurrent RNA encapsulation in vivo . Our strategy is based on...