A5024750951- Sirtuins and Resveratrol in Medicine
- PARP inhibition in cancer therapy
- Calcium signaling and nucleotide metabolism
- Sphingolipid Metabolism and Signaling
- Carbohydrate Chemistry and Synthesis
- Signaling Pathways in Disease
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Plant-derived Lignans Synthesis and Bioactivity
- Tea Polyphenols and Effects
- Autophagy in Disease and Therapy
- Lipid metabolism and biosynthesis
- Biochemical and Molecular Research
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Infective Endocarditis Diagnosis and Management
- Wnt/β-catenin signaling in development and cancer
- Bone Metabolism and Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related gene regulation
- Sports Science and Education
- Cell death mechanisms and regulation
- GDF15 and Related Biomarkers
Kiel University
2023
University of Genoa
2007-2017
Italian Institute of Technology
2014-2017
Agrobioinstitute
2012
Schmerzklinik Kiel
2009
University of Bonn
2000-2006
Cytokine secretion by cancer cells contributes to cancer-induced symptoms and angiogenesis. Studies show that the sirtuin SIRT6 promotes inflammation enhancing TNF expression. Here, we aimed determine whether is involved in conferring an inflammatory phenotype define mechanisms linking inflammation. We enhances expression of pro-inflammatory cyto-/chemokines, such as IL8 TNF, cell migration pancreatic Ca(2+) responses. Via its enzymatic activity, increases intracellular levels ADP-ribose,...
SIRT6 is an NAD(+)-dependent deacetylase with a role in the transcriptional control of metabolism and aging but also genome stability inflammation. Broad therapeutic applications are foreseen for inhibitors, including uses diabetes, immune-mediated disorders, cancer. Here we report on identification first selective inhibitors by silico screening. The most promising leads show micromolar IC50s, have significant selectivity versus SIRT1 SIRT2, active cells, as shown increased acetylation at...
Nicotinamide phosphoribosyltransferase (Nampt) is a key enzyme for nicotinamide adenine dinucleotide (NAD(+)) biosynthesis, and recent evidence indicates its role in inflammatory processes. Here, we investigated the potential effects of pharmacological Nampt inhibition with FK866 mouse myocardial ischemia/reperfusion model. In vivo ex procedures were performed.Treatment reduced infarct size, neutrophil infiltration, reactive oxygen species (ROS) generation within infarcted hearts model...
Summary Pharmacological treatments targeting CXC chemokines and the associated neutrophil activation recruitment into atherosclerotic plaques hold promise for treating cardiovascular disorders. Therefore, we investigated whether FK866, a nicotinamide phosphoribosyltransferase (NAMPT) inhibitor with anti-inflammatory properties that recently found to reduce ischaemic myocardium, would exert beneficial effects in mouse atherosclerosis model. Atherosclerotic plaque formation was induced by...
The fatty acid amide hydrolase (FAAH) regulates the endocannabinoid system cleaving primarily lipid messenger anandamide. FAAH has been well characterized over years and, importantly, it represents a promising drug target to treat several diseases, including inflammatory-related diseases and cancer. But its enzymatic mechanism for selection specifically hydrolyze anandamide, rather than similar bioactive lipids, remains elusive. Here, we clarify this in FAAH, examining role of dynamic...
Systemic and intraplaque biomarkers have been widely investigated in clinical cohorts as promising surrogate parameters of cardiovascular vulnerability. In this pilot study, we if systemic levels calcification were affected by treatment with a statin cohort patients severe carotid stenosis being asymptomatic for ischemic stroke. Patients on therapy had reduced serum osteopontin (OPN), RANKL/osteoprotegerin (OPG) ratio, MMP-9/pro-MMP-9 activity compared to untreated patients. Statin-treated...
Aging | doi:10.18632/aging.100870. Denise Lasigliè, Silvia Boero, Inga Bauer, Sara Morando, Patrizia Damonte, Michele Cea, Fiammetta Monacelli, Paizio Odetti, Alberto Ballestrero, Antonio Uccelli, Raul Mostoslavsky, Alessandro Poggi, Alessio Nencioni
Abstract The ceramides are a family of bioactive lipid‐derived messengers involved in the control cellular senescence, inflammation, and apoptosis. Ceramide hydrolysis by acid ceramidase (AC) stops biological activity these substances influences survival function normal neoplastic cells. Because its central role ceramide metabolism, AC may offer novel molecular target disorders with dysfunctional ceramide‐mediated signaling. Here, class benzoxazolone carboxamides is identified as first...
// Natthakan Thongon 1, * , Ilaria Castiglioni 2, Chiara Zucal 1 Elisa Latorre Vito D'Agostino Inga Bauer 3 Michael Pancher 4 Alberto Ballestrero Georg Feldmann 5 Alessio Nencioni Alessandro Provenzani Laboratory of Genomic Screening, Centre for Integrative Biology, University Trento, Italy 2 Gene Expression and Muscular Dystrophy, San Raffaele Scientific Institute, Milan, Department Internal Medicine, Genoa, High Throughput Screening Facility, Pancreatic Cancer Translational Research,...
We constructed a single-chain variable fragment miniantibody (G11-scFv) directed toward the transactivation domain of c-Myc, which is fused with internalization Int Antennapedia at its carboxyl terminus (a cargo-carrier construct). In ELISA experiments, an EC(50) for binding saturation was achieved concentrations G11-scFv-Int(-) approximately 10(-8) M. Internalization fluoresceinated Fl-G11-scFv-Int(+) construct observed in intact human cultured cells confocal microscopy. After 5 h...
Acid ceramidase (AC) hydrolyzes ceramides, which are central lipid messengers for metabolism and signaling of sphingolipids. A growing body evidence links deregulation sphingolipids to several diseases, including cancer. Indeed, AC expression is abnormally high in melanoma cells. inhibition may thus be key treating malignant melanoma. Here, we have used a systematic scaffold exploration design general pharmacophore inhibition. This comprises 6 + 5 fused ring heterocycle linked an aliphatic...
The potential of plant bioactives for the prevention and therapy diabetes is increasingly being recognized. In present study we investigated antidiabetic properties an aqueous Bistorta officinalis Delarbre extract (BODE) by employing both in-vitro assays in-vivo models. Multiple targets in glucose homeostasis which are involved regulation blood level were affected BODE in-vitro. exhibited inhibitory activities towards intestinal carbohydrate-hydrolysing enzymes α-amylase α-glucosidase with...
Abstract The ceramides are a family of bioactive lipid‐derived messengers involved in the control cellular senescence, inflammation, and apoptosis. Ceramide hydrolysis by acid ceramidase (AC) stops biological activity these substances influences survival function normal neoplastic cells. Because its central role ceramide metabolism, AC may offer novel molecular target disorders with dysfunctional ceramide‐mediated signaling. Here, class benzoxazolone carboxamides is identified as first...
Sirtuins are a family of NAD+-dependent enzymes that was proposed to control organismal life span about decade ago. While such role sirtuins is now debated, mounting evidence involves these in numerous physiological processes and disease conditions, including metabolism, nutritional behavior, circadian rhythm, but also inflammation cancer. SIRT1, SIRT2, SIRT3, SIRT6, SIRT7 have all been linked carcinogenesis either as tumor suppressor or cancer promoting proteins. Here, we review the...