A5100344372- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Synthesis and biological activity
- Veterinary medicine and infectious diseases
- Cell Adhesion Molecules Research
- Synthesis and Biological Evaluation
- Cardiac pacing and defibrillation studies
- RNA regulation and disease
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Synthesis and Characterization of Heterocyclic Compounds
- Atrial Fibrillation Management and Outcomes
- RNA modifications and cancer
- Enzyme Structure and Function
- Cytokine Signaling Pathways and Interactions
- Photochemistry and Electron Transfer Studies
- Carbohydrate Chemistry and Synthesis
- Supramolecular Self-Assembly in Materials
- Cardiac Arrhythmias and Treatments
Pennsylvania State University
2024-2025
Dalian University
2024
Dalian University of Technology
2024
University of California, San Diego
2010-2023
Nankai University
2011-2015
National Taiwan University
2014
South Dakota State University
2010
There has been growing concern that linear ablation is associated with an increased risk of iatrogenic arrhythmias in patients undergoing for atrial fibrillation (AF). Therefore, we compared circumferential pulmonary vein plus left (CPVA+LALA) segmental isolation (PVI)in paroxysmal AF.Sixty-six consecutive AF were prospectively randomly assigned to receive PVI versus CPVA+LALA (consisting encircling lesions around the veins), a roof line, and mitral isthmus line documentation bidirectional...
Many transcription factors contain intrinsically disordered activation domains (TADs), which mediate interactions with coactivators to activate transcription. Historically, DNA-binding and TADs have been considered as modular units, but recent studies shown that can influence DNA binding. Whether these results be generalized more is not clear. Here, we biophysically characterized the NFκB p50/RelA heterodimer including RelA TAD investigated TAD's on NFκB-DNA interactions. In solution, show...
Abstract The intrinsically disordered C-terminal domain (CTD) of RNA polymerase II coordinates transcription and co-transcriptional events through dynamic phosphorylation patterns. While it has been long hypothesized that induces structural changes in the CTD, a direct comparison how different patterns modulate CTD conformation limited. Here, we generated two distinct an essential Drosophila region with kinase Dyrk1a: one where Ser2 are primarily phosphorylated, mimicking state near...
The NFκB transcription factor family members RelA, p50, and cRel form homo- heterodimers that are inhibited by IκBα, IκBβ, IκBε. These have diverse biological functions, their expression profiles differ, leading to different concentrations in tissue types. Here we present definitive biophysical measurements of the dimer affinities inhibitor better understand exchange how presence inhibitors may alter equilibrium dimers cellular context. Fluorescence anisotropy binding experiments were...
Abstract Binding and unbinding of transcription factors to DNA are kinetically controlled regulate the transcriptional outcome. Control release factor NF-κB from is achieved through accelerated dissociation by inhibitor protein IκBα. Using single-molecule FRET, we observed a continuum conformations in free DNA-bound states interconverting on subseconds minutes timescale, comparable vivo binding seconds suggesting that structural dynamics directly control kinetics. Much partially bound,...
ABSTRACT Many transcription factors contain intrinsically disordered activation domains (TADs), which mediate interactions with co-activators to activate transcription. Historically, DNA-binding and TADs have been considered as modular units, but recent studies shown that can influence DNA binding. We biophysically characterized the NFκB p50/RelA heterodimer including RelA TAD investigated TAD’s on NFκB-DNA interactions. In solution is compact, helical tendency in two regions interact...
Abstract Binding and unbinding of transcription factors to DNA are kinetically controlled regulate the transcriptional outcome. Control release factor NF-κB from is achieved through accelerated dissociation by inhibitor protein IκBα. Using single-molecule FRET, we observe a continuum conformations in free DNA-bound states interconverting on subseconds minutes timescale, comparable vivo binding seconds suggesting that structural dynamics directly control kinetics. Much partially bound,...