A5029883352- Cardiac Arrest and Resuscitation
- Respiratory Support and Mechanisms
- Mechanical Circulatory Support Devices
- Traumatic Brain Injury and Neurovascular Disturbances
- Hemoglobin structure and function
- Cardiac Imaging and Diagnostics
- Trauma and Emergency Care Studies
- Thermal Regulation in Medicine
- Neonatal Respiratory Health Research
- Heme Oxygenase-1 and Carbon Monoxide
- Heart Rate Variability and Autonomic Control
- Congenital Heart Disease Studies
- Erythrocyte Function and Pathophysiology
- Sepsis Diagnosis and Treatment
- Congenital Diaphragmatic Hernia Studies
- Cardiac Ischemia and Reperfusion
- Alzheimer's disease research and treatments
- Electron Spin Resonance Studies
- Protein Interaction Studies and Fluorescence Analysis
- Neurological diseases and metabolism
- Cardiac Arrhythmias and Treatments
- Cardiovascular Disease and Adiposity
- Disaster Response and Management
- Prion Diseases and Protein Misfolding
- Assisted Reproductive Technology and Twin Pregnancy
Children's Hospital of Philadelphia
2013-2017
University of Pennsylvania
2013-2014
Philadelphia University
2013-2014
Iowa State University
2004-2006
Stress Abiotiques et Différenciation des Végétaux Cultivés
2006
Banaras Hindu University
2006
University of Wisconsin–Eau Claire
2003
Abstract In the developed world, extreme prematurity is leading cause of neonatal mortality and morbidity due to a combination organ immaturity iatrogenic injury. Until now, efforts extend gestation using extracorporeal systems have achieved limited success. Here we report development system that incorporates pumpless oxygenator circuit connected fetus lamb via an umbilical cord interface maintained within closed ‘amniotic fluid’ closely reproduces environment womb. We show fetal lambs are...
Objectives: During cardiopulmonary resuscitation, adequate coronary perfusion pressure is essential for establishing return of spontaneous circulation. Current American Heart Association guidelines recommend standardized interval administration epinephrine patients in cardiac arrest. The objective this study was to compare short-term survival using a hemodynamic directed resuscitation strategy versus chest compression depth-directed porcine model Design: Randomized interventional study....
Although current resuscitation guidelines are rescuer focused, the opportunity exists to develop patient-centered strategies that optimize hemodynamic response of individual in hopes improve survival.To determine if titrating cardiopulmonary (CPR) blood pressure would 24-hour survival compared with traditional CPR a porcine model asphyxia-associated ventricular fibrillation (VF).After 7 minutes asphyxia, followed by VF, 20 female 3-month-old swine randomly received either pressure-targeted...
Hexacoordinate hemoglobins are a class of proteins that exhibit reversible bis-histidyl coordination the heme iron while retaining ability to bind exogenous ligands. One hypothesis for their physiological function is they scavenge nitric oxide, reaction oxidizes protein and requires reduction continue. Reduction kinetics hexacoordinate hemoglobins, including human neuroglobin cytoglobin, those from Synechocystis rice, compared myoglobin, soybean leghemoglobin, several relevant mutant...
All plants contain an unusual class of hemoglobins that display bis-histidyl coordination yet are able to bind exogenous ligands such as oxygen. Structurally homologous hexacoordinate (hxHbs) also found in animals (neuroglobin and cytoglobin) some cyanobacteria, where they thought play a role free radical scavenging or ligand sensing. The plant hxHbs can be distinguished from the others because only weakly hexcacoordinate ferrous state, no structural mechanism for regulating hexacoordination...
The membrane-active antifungal agent amphotericin B (AmB) is one of the few agents shown to slow course prion diseases in animals. Congo Red and other small molecules have been reported directly inhibit amyloidogenesis both Alzheimer peptide model systems via specific binding. We propose that it possible AmB may act similarly physically prevent conversion largely alpha-helical protein (PrP) pathological beta-sheet aggregate protease-resistant isoform (PrP(res)) disease by analogy...
Effective cardiopulmonary resuscitation is a critical component of the pre-hospital treatment cardiac arrest victims. Mechanical chest compression (MCC) devices enable delivery MCC waveforms that could not be delivered effectively by hand. While generated blood flow has been studied for more than 50 years, relation between sternum kinematics (depth over time) and resulting have well described. Using five parameter model, we effect depth, release time, their interaction on in highly...
Intra-arrest hypothermia induction may provide more benefit than inducing after return of spontaneous circulation. However, little is understood about the interaction between patient physiology and technology choice during ongoing chest compressions. After 10 min untreated ventricular fibrillation, mechanical compressions were provided for 60 (100 CPM, 1.25" deep) in 26 domestic swine (30.5 ± 1.7 kg) with concurrent using one eight cooling methods. Four methods included volume infusion cold...
Introduction: The American Heart Association (AHA) recommends monitoring cardiopulmonary resuscitation (CPR) quality with arterial blood pressure or end tidal carbon dioxide (ETCO2). objective of this study was to evaluate the ability diastolic (DBP) and ETCO2 obtained during late CPR discriminate between survivors non-survivors in porcine models hypoxic normoxic ventricular fibrillation (VF). Methods: In both models, 3-month old swine (~30kg) were randomized receive one two strategies: 1)...
Introduction: Coronary perfusion pressure (CPP) guided CPR improves survival over guideline CPR. EtCO2 has also been used to guide These efforts reflect that one-size-fits-all is sub-optimal, but assume CPP and guidance will result in similar definitions of “high quality” chest compressions (CC). A mechanistic study the interaction between CC, blood flow, CPP, not done. Methods: was performed on 21 domestic swine (~30 Kg) using standard physiological monitoring. Flow measured abdominal...
Introduction: Animal research has suggested that inducing therapeutic hypothermia during the arrest period (e.g. chest compressions) may provide more benefit than after return of spontaneous circulation. Hypothermia induction technologies can be divided into two classes: require volume infusions, and do not. We hypothesize a prolonged cold infusion ongoing CPR will alter hemodynamics, particularly venous blood to heart. Methods: Eighteen temperatures were monitored in 24 domestic swine (~30...
Introduction: Animal research suggests that intra-arrest therapeutic hypothermia, cooling before return of spontaneous circulation (ROSC), may be more protective than inducing hypothermia after ROSC. Reduced blood flow should slow rates, because the amount will determine convective heat transfer. We hypothesize result in significant temperature heterogeneity. Methods: 18 temperatures (7 brain, 4 surface, 7 thoracic and abdominal) were monitored 75 domestic swine (~30 Kg) during induction....