A5011087961- Mycotoxins in Agriculture and Food
- Genomics, phytochemicals, and oxidative stress
- HIV-related health complications and treatments
- Plant Disease Resistance and Genetics
- RNA modifications and cancer
- HIV Research and Treatment
- Phytochemicals and Antioxidant Activities
- Cancer-related gene regulation
- Moringa oleifera research and applications
- Fungal Biology and Applications
- COVID-19 Clinical Research Studies
- Essential Oils and Antimicrobial Activity
- HIV/AIDS Research and Interventions
- Retinoids in leukemia and cellular processes
- Glutathione Transferases and Polymorphisms
- HIV/AIDS drug development and treatment
- Metabolism, Diabetes, and Cancer
- Natural Antidiabetic Agents Studies
- Cancer-related molecular mechanisms research
- HVDC Systems and Fault Protection
- interferon and immune responses
- Plant Stress Responses and Tolerance
- Histone Deacetylase Inhibitors Research
- Health, Environment, Cognitive Aging
- Selenium in Biological Systems
University of KwaZulu-Natal
2017-2025
University of Zululand
2017
Fusaric acid (FA) is a food-borne mycotoxin that mediates toxicity with limited information on its epigenetic properties. p53 tumour suppressor protein regulates cell cycle arrest and apoptotic death. The expression of regulated transcriptionally by promoter methylation post-transcriptionally N-6-methyladenosine (m6A) RNA methylation. We investigated the effect FA regulation via m6A in human hepatocellular carcinoma (HepG2) cells. HepG2 cells were treated [0, 25, 50, 104, 150 µg/ml; 24 h]...
Methylenetetrahydrofolate reductase (MTHFR) gene is involved in homocysteine and folic acid metabolism. Tumour suppressor protein TP53 maintains cellular genetic integrity. To date, no studies associated the MTHFR C677T rs1801133 Pro72Arg rs1042522 with CRP levels methotrexate (a antagonist) treatment outcomes psoriatic arthritis (PsA) patients. The present study aimed to investigate whether variants influences South African Indian Caucasian PsA patients (n=114) healthy controls (n=100) were...
Fusaric acid (FA), a common fungal contaminant of maize, is known to mediate toxicity in plants and animals; however, its mechanism action unclear. p53 tumor suppressor protein that activated response cellular stress. The function regulated by post-translational modifications-ubiquitination, phosphorylation, acetylation. This study investigated possible FA induced the human hepatocellular carcinoma (HepG2 ) cell line. effect on DNA integrity modifications were investigated. Methods included:...
Fumonisin B1 (FB1), a Fusarium-produced mycotoxin, is found in various foods and feeds. It well-known liver carcinogen experimental animals; however, its role genotoxicity controversial. The current study investigated FB1-triggered changes the epigenetic regulation of PTEN determined effect on DNA damage checkpoint human hepatoma G2 (HepG2) cells. Following treatment with FB1 (IC50: 200 µM; 24 h), expression miR-30c, KDM5B, PTEN, H3K4me3, PI3K, AKT, p-ser473-AKT, CHK1, p-ser280-CHK1 was...
Fumonisin B1 (FB1) is a natural contaminant of agricultural commodities that has displayed myriad toxicities in animals. Moreover, it known to be hepatorenal carcinogen rodents and may associated with oesophageal hepatocellular carcinomas humans. The most well elucidated mode FB1-mediated toxicity its disruption sphingolipid metabolism; however, enhanced oxidative stress, endoplasmic reticulum autophagy, alterations immune response also play role carcinogenicity. Alterations the host...
Fusaric acid (FA), a mycotoxin contaminant of maize, displays toxicity in plants and animals; however, its epigenetic mechanism is unknown. DNA methylation, an modification that regulates gene expression, mediated by methyltransferases (DNMTs; DNMT1, DNMT3A, DNMT3B) demethylases (MBD2). The expression DNMTs are regulated promoter microRNAs (miR-29b) post-translational modifications (ubiquitination). Alterations these methylation modifying enzymes affect patterns offer novel mechanisms FA...
Ubiquitous soil fungi parasitise agricultural commodities and produce mycotoxins. Fumonisin B2 (FB2), the structural analogue of commonly studied B1 (FB1), is a neglected mycotoxin produced by several Fusarium species. Mycotoxins are known for inducing toxicity via mitochondrial stress alluding to degradation (mitophagy). These processes involve inter-related pathways that regulated proteins related SIRT3 Nrf2. This study aimed investigate responses in human kidney (Hek293) cells exposed FB2...
The World Health Organization (WHO) reported that there are 37 million individuals living with the human immunodeficiency virus (HIV) worldwide, majority in South Africa. This chronic disease is managed by effective use of antiretroviral (ARV) drugs. However, prolonged use, ARV drug-induced toxicity remains a clinically complex problem. study investigated drugs on mitochondria and NRF2 antioxidant pathway its possible amelioration using Moringa oleifera Lam (MO) leaf extracts. medicinal...
The cosmetic industry makes extensive use of kojic acid (KA); however, the toxicity KA in humans is not well known. By monitoring oxidative stress, mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NFκB) signalling human hepatoma (HepG2) cells after a 24 h exposure, this study aimed to identify KA. [4.22, 8.02 12.67 mM] was assessed using mitochondrial output, antioxidant responses, macromolecule damage, MAPK signalling, inflammation, cell death markers, spectrophotometry,...
Psoriasis and psoriatic arthritis (PsA) are inflammatory associated autoimmune disorders. MicroRNA (miR)-146a plays a crucial role in regulating inflammation. A single nucleotide polymorphism the miR-146a gene (rs2910164), aberrantly alters its expression linked with pathogenesis of several disorders, including psoriasis PsA. In South Africa, PsA extremely rare indigenous African population most common both Indian Caucasian population. The aim this study was to investigate whether rs2910164...