A5101924696- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Herpesvirus Infections and Treatments
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Ocular Diseases and Behçet’s Syndrome
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal and Optic Conditions
- Photochromic and Fluorescence Chemistry
- Barrier Structure and Function Studies
- 3D Printing in Biomedical Research
- Neurological Disease Mechanisms and Treatments
- Retinopathy of Prematurity Studies
- Hepatitis Viruses Studies and Epidemiology
- interferon and immune responses
- Viral Infections and Immunology Research
- Immune Response and Inflammation
- Corneal Surgery and Treatments
- Cancer Research and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- NF-κB Signaling Pathways
Yale University
2016-2024
Medical University of South Carolina
2012-2016
Columbia University Irving Medical Center
2009-2012
Columbia University
2009-2011
Augusta University
2003-2010
Med Center
2009
Modeling age-related macular degeneration (AMD) is challenging, because it a multifactorial disease. To focus on interactions between the retinal pigment epithelium (RPE) and Bruch's membrane, we generated RPE from AMD patients used an altered extracellular matrix (ECM) that models aged membrane. Induced pluripotent stem cells (iPSCs) were fibroblasts isolated or age-matched (normal) controls. derived iPSCs analyzed by morphology, marker expression, transepithelial electrical resistance...
Engagement of the T cell antigen receptor (TCR) rapidly induces multiple signal transduction pathways, including ERK activation. Here, we report a critical role for at late stage Inhibition pathway 2–6 h after start TCR stimulation significantly impaired interleukin-2 (IL-2) production, whereas same treatment during first 2 had no effect. inhibition nuclear translocation c-Rel with minimum reduction NF-AT activity. Requirement sustained activation was also confirmed using primary cells. To...
To determine the effects of age and topographic location on gene expression in human neural retina.Macular peripheral retina RNA was isolated from donor eyes for DNA microarray quantitative RT-PCR analyses.Total integrity donors preserved. Hierarchical clustering analysis demonstrates that profiles young, old, macula, cluster into four distinct groups. Genes which are highly expressed macular, peripheral, or old were identified, including inhibitors Wnt Signaling Pathway (DKK1, FZD10, SFRP2)...
Purpose We have shown previously that non-enzymatic nitration (NEN) of the extracellular matrix (ECM), which serves as a model Bruch's membrane (BM) aging, has profound effect on behavior overlying retinal pigment epithelial (RPE) cells, including altered phagocytic ability, reduced cell adhesion, and inhibition proliferation. know transplanted RPE monolayers will encounter hostile sub-RPE environment, age-related alterations in BM may compromise function survival. Here we use our previous...
We have shown previously that Bruch's membrane (BM) aging decreases retinal pigment epithelium (RPE) phagocytosis. Herein, we determine the effects of BM reengineering on RPE phagocytosis.BM explants were dissected from young and old donor eyes. Some reengineered by cleaning with Triton X-100 and/or coating extracellular matrix (ECM) ligands. ARPE-19 cell-derived ECM (ARPE-ECM) modified ("aged") sodium nitrite was subjected to similar treatments. cells then cultured confluence onto different...
Compared with many induced pluripotent stem cell (iPSC) lines generated using retrovirus and other non-integrating methods, the utilization of human protein-induced iPSC (piPSC) may provide a safer alternative for generation retinal pigment epithelial (RPE) cells transplantation in degenerative diseases. Here we assess ability piPSCs to differentiate into RPE cells, perform native behavior. were seeded 6-well low-attachment plates allow embryoid body formation, then analyzed markers NANOG,...
purpose. To determine whether infiltrating polymorphonuclear leukocytes PMNs play a role in preventing early direct anterior-to-posterior spread of herpes simplex virus (HSV)-1 and/or the HSV-1 from brain back to retina injected eye after anterior chamber (AC) inoculation. methods. BALB/c mice were treated with monoclonal antibody RB6-8C5 (Gr-1) against or control IgG and inoculated HSV-1. results. In Gr-1–treated mice, depleted peripheral blood HSV-1–infected eye. More (2–3 logs) was...
Purpose: Loss of CD46 has recently been implicated in choroidal neovascularization mice. Herein we investigated the effect nitrite modification extracellular matrix (ECM) as an vitro model "aging" and its on expression vascular endothelial growth factor (VEGF) release cocultured human retinal pigment epithelium (RPE). Methods: ARPE-19 cells were plated onto RPE-derived ECM conditions (untreated; modified; modified followed by washing with Triton X-100; or X-100 coated ligands). Cells...
Aside from vitamins and antioxidants recommended by the Age-Related Eye Disease Study, there is no effective therapy for "dry," or atrophic age-related macular degeneration (AMD) which represents 90% of cases. Therapies are needed to slow retard development geographic atrophy (GA), understanding Bruch's membrane pathology part this process. Alterations in human precede progression AMD contributing damage retinal pigment epithelial (RPE) cells. Given lack sufficient animal models study AMD,...
After uniocular anterior chamber (AC) inoculation with HSV-1, the segment of injected eye becomes inflamed and infected; however, virus does not spread from infect retina eye. The purpose this study was to identify early infiltrating cells determine whether produce interferon (IFN)gamma.Euthymic, female, BALB/c mice were in one AC 3 x 10(4) PFU HSV-1 (KOS) a volume 2 microL. Mice each group killed at 12, 24, 36, 48, 72 hours post injection (pi), eyes enucleated, frozen sections stained...
Tumor necrosis factor alpha (TNF-alpha) has been shown to have a protective role in the eyes and brains of herpes simplex virus type 1 (HSV-1)-infected mice. To determine whether overexpression TNF-alpha affected course infection following uniocular anterior chamber inoculation, recombinant HSV-1 that produces constitutively (KOSTNF) was constructed. BALB/c mice were injected with recombinant, containing pCI plasmid, rescue virus, or parental virus. Flow cytometry immunohistochemistry used...
We have reported previously that retinal pigment epithelium (RPE) differentiated from induced pluripotent stem cells (iPSC) generated fibroblasts of patients with age-related macular degeneration (AMD) exhibit a degenerative disease phenotype and distinct transcriptome compared to age-matched controls. Since the genetic composition iPSC RPE are inherited fibroblasts, we investigated whether differential behavior was present in parental prior differentiation cell lines into RPE. Principal...
Aside from vitamins and antioxidants recommended by the Age-Related Eye Disease Study, there is no effective therapy for "dry," or atrophic age-related macular degeneration (AMD) which represents 90% of cases. Therapies are needed to slow retard development geographic atrophy (GA), understanding Bruch's membrane pathology part this process. Alterations in human precede progression AMD contributing damage retinal pigment epithelial (RPE) cells. Given lack sufficient animal models study AMD,...