A5036436844- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- DNA and Nucleic Acid Chemistry
- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Nicotinic Acetylcholine Receptors Study
- Neurotransmitter Receptor Influence on Behavior
- Advanced biosensing and bioanalysis techniques
- Olfactory and Sensory Function Studies
- Multisensory perception and integration
- Neuroscience and Music Perception
- CRISPR and Genetic Engineering
- Electron Spin Resonance Studies
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
- Metabolism and Genetic Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Medical Image Segmentation Techniques
- Pluripotent Stem Cells Research
Leibniz Institute for Neurobiology
2006-2019
Center for Behavioral Brain Sciences
2012-2019
Otto-von-Guericke University Magdeburg
2012
Max Planck Institute for Biophysical Chemistry
2003
Institute of Neurobiology
1986-1991
Recent data from animal studies raise the possibility that dopaminergic neuromodulation promotes encoding of novel stimuli. We investigated a possible role for midbrain in human episodic memory by measuring how polymorphisms dopamine clearance pathways affect encoding-related brain activity (functional magnetic resonance imaging) an task. In 51 young, healthy adults, successful was associated with activation substantia nigra. This modulated functional variable number tandem repeat (VNTR)...
Abstract New memories initially persist in a labile state and require protein synthesis‐dependent processes of consolidation for long‐term manifestation. Using differential conditioning to linearly frequency‐modulated tones (FMs) we have recently shown that post‐training injections synthesis inhibitors into the auditory cortex Mongolian gerbils interfere with memory number days. Here, used rapamycin as pharmacological tool elucidate signalling pathways control proteins required persistent...
Previous studies in the auditory cortex of Mongolian gerbils on discrimination learning direction frequency-modulated tones (FMs) revealed that long-term memory formation involves activation dopaminergic system, activity protein kinase mammalian target rapamycin (mTOR), and synthesis. This led to hypothesis system might modulate via regulation mTOR, which is implicated translational control. Here, we report D1/D5 dopamine receptor agonist SKF-38393 substantially improved gerbils' FM when...
In Mongolian gerbils, the auditory cortex is critical for discriminating rising vs. falling frequency-modulated tones. Based on our previous studies, we hypothesized that dopaminergic inputs to during and shortly after acquisition of discrimination strategy control long-term memory formation. To test this hypothesis, studied tone learning gerbils in a shuttle box GO/NO-GO procedure following differential treatments. (i) Pre-exposure tones at 1 day before first training session severely...
Recently, the induction of transcription factor-encoding immediate-early genes such as c-fos was observed in distinct brain regions rats trained to acquire a footshock-motivated brightness discrimination Y-maze. The functional relevance inducible factors for learning and memory formation is, however, not clear. To address this question present study, we have used synthetic antisense phosphorothioate oligodeoxynucleotide suppress vivo expression rat brain. Intrahippocampal application 10 hr 2...
Hybridization studies revealed a differential accumulation of c-jun and jun B mRNA in the hippocampus cerebral cortex rats trained on foot-shock-motivated brightness discrimination. Supposing that early gene expression is functionally significant for plastic changes brain, we used antisense phosphorothioate oligodeoxynucleotides (S-ODN) vivo to study effects specific inhibition learning memory formation rat brain. Discrimination performance was impaired after intrahippocampal injection...
Bassoon is a large scaffolding protein of the presynaptic active zone involved in development terminals and regulation neurotransmitter release at both excitatory inhibitory brain synapses. Mice with constitutive ablation (Bsn) gene display impaired function, show sensory deficits develop severe seizures. To specifically study role forebrain synapses its relevance for control behavior, we generated conditional knockout (Bsn cKO) mice by through an Emx1 promoter-driven Cre recombinase. In...
Differential conditioning of Mongolian gerbils to linearly frequency-modulated tones (FM) has recently received experimental attention. In the study role cerebral protein synthesis for FM discrimination memory, post-training bilateral injections anisomycin into auditory cortex under light halothane anesthesia. Compared with saline-treated controls, anisomycin-treated showed a decrement during subsequent three days training. They markedly improved their performance within training sessions,...
Using auditory discrimination learning in gerbils, we have previously shown that activation of auditory-cortical D1/D5 dopamine receptors facilitates mTOR-mediated, protein synthesis-dependent mechanisms memory consolidation and anterograde formation. To understand molecular this facilitatory effect, tested the impact local pharmacological different receptor signalling modes cortex. end, patterns soluble synaptic protein-enriched fractions from cortical, hippocampal striatal brain regions...
Abstract Learning and memory processes are accompanied by rearrangements of synaptic protein networks. While various studies have demonstrated the regulation individual proteins during these processes, much less is known about complex proteomes. Recently, we reported that auditory discrimination learning in mice associated with a relative down‐regulation involved structural organization synapses brain regions. Aiming at identification biological signaling pathways formation, here, label‐free...
Changes in synaptic efficacy underlying learning and memory processes are assumed to be associated with alterations of the protein composition synapses. Here, we performed a quantitative proteomic screen monitor changes proteome four brain areas (auditory cortex, frontal hippocampus striatum) during auditory learning. Mice were trained shuttle box GO/NO-GO paradigm discriminate between rising falling frequency modulated tones avoid mild electric foot shock. Control-treated mice received...
The molecular synaptic mechanisms underlying auditory learning and memory remain largely unknown. Here, the workflow of a proteomic study on discrimination in mice is described. In this paradigm, are trained shuttle box Go/NoGo-task to discriminate between rising falling frequency-modulated tones order avoid mild electric foot-shock. protocol involves enrichment synaptosomes from four brain areas, namely cortex, frontal hippocampus, striatum, at different stages training. Synaptic protein...