A5100772095- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Plant Pathogens and Fungal Diseases
- Tendon Structure and Treatment
- Genomics and Phylogenetic Studies
- Spinal Fractures and Fixation Techniques
- Plant Disease Resistance and Genetics
- Osteoarthritis Treatment and Mechanisms
- Actinomycetales infections and treatment
- Video Analysis and Summarization
- Medical Imaging and Analysis
- Orthopaedic implants and arthroplasty
- Circular RNAs in diseases
- Cervical and Thoracic Myelopathy
- Scoliosis diagnosis and treatment
- Microbial Natural Products and Biosynthesis
- Phytochemical Studies and Bioactivities
- Advanced biosensing and bioanalysis techniques
- Spinal Hematomas and Complications
- Ocular Diseases and Behçet’s Syndrome
- MicroRNA in disease regulation
- Generative Adversarial Networks and Image Synthesis
- Library Science and Information Systems
- Marine and Coastal Research
- Spinal Dysraphism and Malformations
Gunma University
2025
Alibaba Group (United States)
2024
Center for Life Sciences
2024
Peking University
2024
Soochow University
2023
Xijing Hospital
2009-2021
People 's Liberation Army 451 Hospital
2014-2020
Air Force Medical University
2009-2020
Liaocheng People's Hospital
2019
Tianjin Medical University
2012
The role of apoptosis in the pathogenesis intervertebral disc degeneration (IDD) remains enigmatic. Accumulating evidence has shown that apoptotic machinery is regulated by miRNAs. We hypothesized miRNAs might contribute to IDD. have found 29 were differentially expressed and miR-155 was down-regulated degenerative nucleus pulposus (NP). deregulation further verified using real-time PCR (0.56 fold, p < 0.05). Bioinformatics target prediction identified FADD caspase-3 as putative targets...
Accumulating evidence indicates noncoding RNAs (ncRNAs) fine-tune gene expression with mysterious machinery. We conducted a combination of mRNA, miRNA, circRNA, LncRNA microarray analyses on 10 adults' lumbar discs. Moreover, we performed additional global exploration RNA interacting machinery in terms silico computational pipeline. Here show the landscape human In general, RNA-abundant comprises 14,635 mRNAs (37.93%), 2,059 miRNAs (5.34%), 18,995 LncRNAs (49.23%) and 2,894 (7.5%) circRNAs....
Abstract Background Notwithstanding the clinical hallmarks of COVID-19 patients were reported, several critical issues still remain mysterious, i.e., prognostic factors for including extrinsic as viral load SARS-CoV-2 and intrinsic individual’s health conditions; myocarditis incidence rate hallmarks. Methods Demographic, epidemiologic, radiologic laboratory data collected by medical record reviews adult hospitalized diagnosed COVID-19. Cycle threshold (Ct) value real-time PCR (RT-PCR)...
Angiogenesis is a pathological signature of intervertebral disc degeneration (IDD). Accumulating evidence has shown that notochordal cells (NCs) play an essential role in maintaining development and homeostasis with inhibitive effect on blood vessel in-growth. However, the anti-angiogenesis mechanism NCs still unclear. In current study, we, for first time, isolated NC-derived exosomes (NC-exos) showed their increased concentration following compressive load cultures. We further found NC-exos...
The mechanisms of immune privilege in human nucleus pulposus (NP) remain unclear.Accumulating evidence indicates that Fas ligand (FasL) might play an important role the disc.We aimed for addressing FasL expression intervertebral disc degeneration (IDD) and terms interaction between NP cells immunocytes via FasL-Fas machinery.We collected specimens from 20 patients with IDD as degenerative group 8 normal cadaveric donors control.FasL was detected by qRT-PCR, western blotting flow cytometry...
Accumulating evidence indicates that noncoding RNAs play important roles in a multitude of biological processes. The striking findings miRNAs (microRNAs) and lncRNAs (long RNAs) as members open up an exciting era the studies gene regulation. More recently, reports circRNAs (circular add fuel to research. Human intervertebral disc degeneration (IDD) is main cause low back pain disabling spinal disease. We have addressed expression profiles if miRNAs, mRNAs IDD (Wang et al., J Pathology, 2011...
Introduction:Abnormal biomechanics plays a role in intervertebral disc degeneration.Adipose-derived stromal cells (ADSCs) have been implicated integrity; however, their the setting of mechanical stimuli upon disc's nucleus pulposus (NP) remains unknown.As such, present study aimed to evaluate influence ADSCs NP compressive load culture.Methods: Human were cultured at 3.0MPa for 48 hours with or without co-culture (the ratio was 50:50).We used flow cytometry, live/dead staining and scanning...
Acute myocardial infarction (AMI) is a common cardiovascular disease with high disability and mortality. Circular RNAs (circRNAs) are implicated in the pathomechanism of multiple human diseases, including AMI. This study intended to explore function working mechanism novel circRNA circ_0023461 hypoxia-induced cardiomyocytes.Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) Western blot assay were implemented detect RNA protein expression. Cell counting kit-8 (CCK8)...
Human brains respond to semantic features of presented stimuli with different neurons. It is then curious whether modern deep neural networks admit a similar behavior pattern. Specifically, this paper finds small cluster neurons in diffusion model corresponding particular subject. We call those the concept They can be identified by statistics network gradients stimulation connected given The demonstrate magnetic properties interpreting and manipulating generation results. Shutting them...
Rapid and sensitive detection of virus-related antigens antibodies is crucial for controlling sudden seasonal epidemics monitoring neutralizing antibody levels after vaccination. However, conventional methods still face challenges related to compatibility with rapid, highly sensitive, compact apparatus. In this work, we developed a Si nanowire (SiNW)-based field-effect biosensor by precisely the process conditions achieve required electrical properties via complementary...
Existing end-to-end speech large language models (LLMs) usually rely on large-scale annotated data for training, while data-efficient training has not been discussed in depth. We focus two fundamental problems between and text: the representation space gap sequence length inconsistency. propose Soundwave, which utilizes an efficient strategy a novel architecture to address these issues. Results show that Soundwave outperforms advanced Qwen2-Audio translation AIR-Bench tasks, using only...
African swine fever (ASF) is a highly contagious, fatal disease of pigs caused by virus (ASFV). The complexity ASFV and our limited understanding its interactions with the host have constrained development vaccines antiviral strategies. To identify factors required for replication, we developed genome-wide CRISPR knockout (GeCKO) screen that contains 186,510 specific single guide RNAs (sgRNAs) targeting 20,580 pig genes used genotype II to perform GeCKO in wild boar lung (WSL) cells. We...
The 16S rDNA sequences of four strains, i.e. three type strains Actinobispora and strain 6330T, were determined compared with those representatives the family Pseudonocardiaceae by using two tree-making algorithms. All validly described species genera Pseudonocardia consistently recovered as a mixed group in phylogenetic trees, distinct from other Pseudonocardiaceae. Strain 6330T formed phyletic line tree was most closely associated aurantiaca. use specific PCR primers designed for...
As an intermediate filament protein, cytokeratin 8 (CK8) exerts multiple cellular functions.Moreover, it has been identified as a marker of notochord cells, which play essential roles in human nucleus pulposus (NP).However, the distribution CK8 positive cells NP and their relationship with intervertebral disc degeneration (IDD) have not clarified until now.Here, we found percentage IDD (25.7±4.14%) was significantly lower than that normal scoliosis (51.9±9.73% 47.8±5.51%,respectively,...
ABSTRACT Biologic and cellular treatment strategies aiming for curing intervertebral disc degeneration (IDD) have been proposed recently. Given the convenient availability expansion potential, adipose‐derived stromal cells (ADSCs) might be an ideal cell candidate. However, interaction between ADSCs nucleus pulposus (NP) still remains ambiguous, especially in direct co‐cultures of two types cells. Nevertheless, NP markers after were unidentified. Here, we addressed human a co‐culture system...
Objective: As a main cellular component within the disc, nucleus pulposus (NP) cells play important roles in disc physiology.However, little is known on biologic hallmarks of human NP cells.Therefore, present study aimed to address features cells.Methods: Human samples were collected from normal cadavers, patients with scoliosis and degeneration as normal, disease control degenerative NP, respectively.The studied using transmission electron microscopy TUNEL assay.Pre-digested flow cytometry...