Summary. The changes in the plasma levels of progesterone, unconjugated oestrone and oestradiol-17 β, sulphate sow at time implantation, during gestation parturition are described. progesterone concentration remained fairly constant (10 to 12 ng/ml) throughout pregnancy until 20 15 days before parturition. dropped over last gestation. Measurable quantities were present maternal Day 16 but not 9 rose a peak >3 ng/ml (assessed as free oestrone) between 23 30. fell low level 35 pg/ml on 46...

SUMMARY A method is described for diagnosing the presence or absence of a conceptus soon after mating insemination. It applicable to wide range mammals and tests in cow, sheep pig have given an accuracy approximately 90%. The based on detection functional regressed corpus luteum at fixed time by measuring concentration progesterone peripheral blood. relatively simple carry out.

The onset of ovarian function and occurrence estrus were monitored in two groups Holstein cows over the first 90 days post-partum. Group 1 consisted 36 animals housed a free stall area observed continuously for with closed circuit television time lapse videorecorder. 2 33 tie stalls detected by herdsmen. Blood samples collected twice weekly assayed progesterone. plasma progesterone profiles used to monitor determine ovulation time. In both there was close agreement between basal...

Summary. The growth and atresia of individual Graafian follicles in the ovaries fourteen cycling Suffolk ewes were followed vivo by marking large follicle(s) present with Indian ink at three different times during oestrous cycle. containing marked removed on 3rd to 9th day next cycle sectioned determine whether had ovulated. results showed that before last regressed did not ovulate. Only at, or just before, onset oestrus, ovulated developed into corpora lutea. It is suggested there are least...

Summary. The changes in concentration of oestrone, oestradiol (-17α and -17β), oestrone sulphate the sulphates have been measured allantoic amniotic fluids maternal peripheral plasma throughout gestation. Oestrone was major present all fluids. It measurable fluid before Day 52 reached a peak 475 ng/ml around 133. A lower occurred 110. were similar to those but at much level. Considerable fluctuation observed concentrations fluid. ratio oestradiol-17α oestradiol-17β considerably higher than...

Ovine placental lactogen (oPL), has been purified approximately 1,000-fold from sheep cotyledons using conventional protein purification procedures. Radioreceptor assays rabbit liver particulate fractions for growth hormone (RRA-GH) and mammary gland prolactin (RRA-PRL) were employed to monitor the hormonal activities. The molecular weight of oPL is 22,000 as determined by gel filtration on Sephadex G-100, its isoelectric point focusing 8.8. In two RRA's, displacement curve parallel bovine...

The concentration of the unconjugated and sulfoconjugated estrogens have been measured in fetal maternal fluids ewe throughout pregnancy. Estrone sulfate was major estrogen all examined. In fluids, it first detectable allantoic fluid on Day 31, peaked at a high level (14.2 ng/ml) 46 declined rapidly to 850 pg/ml 55 before gradually rising second level. amniotic followed that fluid, but no peak evident. pattern for estrone similar sulfoconjugate, very much lower change estradiol sulfates...

Nulliparous heifers (N = 21) were killed at each day between Days 20 and 29 after insemination. Placentomes collected from the region near embryo prepared for examination by light electron microscopy. At Day placentomes could be recognized as discrete oval structures on surface of uterine lumen. 20-21, trophoblast was in close apposition to caruncular epithelium with microvilli maternal cells indenting apical border embryonic cells. Mutual interdigitation present 24 this progressed intimate...

A specific and sensitive homologous RIA for ovine placental lactogen (oPL) has been developed. The assay is oPL in that pituitary PRL (oPRL), GH (oGH), other hormones, as well rat, caprine, bovine, monkey, human PLs exhibit no cross-reaction the assay. lower limit of sensitivity 1.0 ng/ml. In uterine vein samples was measurable after day 40 gestation peripheral sera 48 (3.5 +/- 1.9 ng/ml). Peripheral plasma concentrations reached peak levels by 131--141 (649 205--565 347 ng/ml), then began...

The progesterone concentration in peripheral plasma has been measured sequentially individual cows during the estrous cycle, gestation, at parturition, and post-partum. During cycle was lowest just before, during, after estrus when level (0.1–0.4 ng/ml) similar to that found three ovariectomized cows. commenced rise on 4th–6th day (day of = 1st day), reached a peak 3–6 ng/ml 11th–13th day, dropped rapidly over 24–48-hr period basal value 24–72 hr before next estrus. In early pregnancy,...

Nulliparous heifers were killed, 3\p=n-\5each day, at 3-day intervals between Days 30 and 45 after insemination 4 additional animals killed 48 67.Placentomes collected from the region near fetus prepared for examination by light electron microscopy.At days placentomes visible on internal surface of fixative-treated uteri as discrete, oval structures with a lighter appearance than surrounding tissues.The maternal\p=n-\trophoblast interface ranged almost completely flat to slightly undulating...

The changes in concentration of unconjugated oestrone, oestradiol-17\m=infty\and oestradiol-17\g=b\ have been measured the maternal jugular venous plasma pregnant cows over a period from 40 days before to 6 after parturition.Measurable levels these oestrogens were present parturition.A steady increase their commenced 20 parturition and peak 4\m=.\5ng/ml 300 pg/ml for 450 found just parturition.The all three dropped dramatically first 24 hr and, by 48 hr, concentrations had <15 oestrone <35...

Summary. Specimens were obtained from the uteri of heifers on Days 17, 18, 20 to 30 and at 3-day intervals up Day 45 gestation this material was examined by light microscopy, transmission scanning electron microscopy. A reduction in height uterine epithelium 18 first detectable maternal response presence blastocyst within lumen. The trophoblast could be preserved contact with attachment became progressively more complex between 21 30. Attachment occurred simultaneously intercaruncular...

Specific binding sites for ovine placental lactogen (oPL) have been detected in the 100,000 × g pellet obtained from tissues derived non-pregnant and pregnant ewes as well fetal tissues. of [125I]iodo-PL (percentage per milligram protein) was found ewe liver (28%), adipose tissue (20%), ovary (10%), corpus luteum (6%), uterus (5%), (5%). The specific oPL less than 3% following maternal tissues: mammary gland, adrenal, kidney, spleen, pancreas, lung, heart, skeletal muscle brain (cortex),...

Dementia is an emerging health priority in Australian Aboriginal communities, but substantial gaps remain our understanding of this issue, particularly for the large urban section population. In remote high prevalence rates dementia at relatively young ages have been reported. The current study investigating aging, cognitive decline, and older urban/regional Australians.

Despite growing interest in river and catchment restoration, including a focus on nature-based solutions, assessing effectiveness of restoration programmes continues to prove challenge. The development the Eddleston Water project, Scottish Government’s empirical study impact implementing natural flood management measures risk habitat provides opportunity review monitoring at strategic operational level for this long-running programme. project has implemented an extensive range along across...

Background/Objectives: Antimicrobial resistance (AMR) is a global problem with antibiotic consumption considered key modifiable factor for the development of AMR. Long-term care (LTC) facilities have been identified as potential reservoirs Escherichia coli (E. coli) due to high rates urinary tract infection (UTI) and levels among residents. However, while relationship between these two factors well accepted, little known about possible temporal these. This study explores trends in E. LTC...

Background: Antimicrobial resistance (AMR) is a global health challenge with significant variation. Little known about the prevalence on smaller geographical scale. Objectives: This study aimed to explore geo-temporal variation in antibiotic Escherichia coli (E. coli) urinary isolates Illawarra Shoalhaven region, region south of Sydney. Methods: Data from urine E. people living community were geospatially analysed 2008 2018. The proportion resistant was mapped by type (amoxicillin clavulanic...

Detectable concentrations of oestrone sulphate were present in 50% the plasma samples collected from pregnant animals by Day 17. No was detected cyclic nonpregnant pigs.

Sows and ewes were killed at various times during gestation. Minced uterine samples incubated for 3 h with [6,7-3H]oestrone sulphate or [2,4,6,7-3H]oestrone, respectively. Contrasting patterns of enzyme activities found in the sow ewe. In sow, endometrial sulphotransferase activity declined after Day 30 gestation, while ewe, sulphation gradually increased 16. The inverse was oestrogen sulphatase pregnancy.