A5039722314- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Supramolecular Self-Assembly in Materials
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Dendrimers and Hyperbranched Polymers
- Polymer Surface Interaction Studies
- Advanced Polymer Synthesis and Characterization
- biodegradable polymer synthesis and properties
- Monoclonal and Polyclonal Antibodies Research
- Luminescence and Fluorescent Materials
- Molecular Junctions and Nanostructures
- Pickering emulsions and particle stabilization
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- DNA and Nucleic Acid Chemistry
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Nanocluster Synthesis and Applications
- Photochromic and Fluorescence Chemistry
- Cancer therapeutics and mechanisms
- Gold and Silver Nanoparticles Synthesis and Applications
- Esophageal Cancer Research and Treatment
- Polydiacetylene-based materials and applications
- Graphene and Nanomaterials Applications
Max Planck Institute for Polymer Research
2013-2025
ARUP Laboratories (United States)
2024
University of Utah
2023-2024
Cedars-Sinai Medical Center
2023
Division of Cancer Epidemiology and Genetics
2023
Cancer Genetics (United States)
2023
Cedars-Sinai Smidt Heart Institute
2023
Cold Spring Harbor Laboratory
2022
Universität Ulm
2012-2021
University of Applied Sciences Mainz
2018-2020
The conjugation of synthetic polymers with various biomolecules provides an easy access to biohybrid materials which combine advantages from both the world and Nature. Due rapid development tools deepening understanding biomolecule structure function, these polymer bioconjugates are not only important for biomedical applications, but also can serve as innovative constructs in science. This review summarizes a selection structurally defined their application building blocks preparing...
A versatile, bottom-up approach allows the controlled fabrication of polydopamine (PD) nanostructures on DNA origami. PD is a biosynthetic polymer that has been investigated as an adhesive and promising surface coating material. However, control dopamine polymerization challenged by multistage-mediated reaction mechanism diverse chemical structures in PD. origami decorated with multiple horseradish peroxidase-mimicking DNAzyme motifs was used to shape size formation nanometer resolution....
Synthetic assembly within living cells represents an innovative way to explore purely chemical tools that can direct and control cellular behavior. We use a simple modular platform is broadly accessible yet incorporates highly intricate molecular recognition, immolative, rearrangement chemistry. Short bimodular peptide sequences undergo programmed sequence of events be tailored the intracellular environment. Each sequential stage pathways beginning with uptake, transport, localization...
The polymerization of biomolecules is a central operation in biology that connects molecular signals with proliferative and information-rich events cells. As molecules arrange precisely across 3-D space, they create new functional capabilities such as catalysis transport highways exhibit phase separation phenomena fuel nonequilibrium dynamics Hence, the observed polymer chemistry manifests itself basis leading to cellular phenotypes, expressed multitude hierarchical structures found cell...
Single-molecule localization microscopy (SMLM) is a powerful technique to achieve super-resolution imaging beyond the diffraction limit. Although various types of blinking fluorophores are currently considered for SMLM, intrinsic remain rare at single-molecule level. Here, we report synthesis nanographene-based burst-blinking highly versatile SMLM. We image amyloid fibrils in air and pH solutions without any additive lysosome dynamics live mammalian cells under physiological conditions. In...
The efficient conjugation of a ruthenium complex and the peptide hormone somatostatin is presented. resultant biohybrid offers valuable features for photodynamic therapy such as remarkable cellular selectivity, rapid cell uptake by receptor-mediated endocytosis, generation (1)O2 upon irradiation, potent phototoxicity well low cytotoxicity in "off"-state.
The development of protein–polymer hybrids emerged several decades ago with the vision that their synergistic combination will offer macromolecular manifold features to succeed as next generation therapeutics.
The modulation of protein uptake and activity in response to physiological changes forms an integral part smart therapeutics. We describe herein the self-assembly a pH-responsive dendrimer shell onto surface active enzymes (trypsin, papain, DNase I) as supramolecular protecting group form hybrid dendrimer-enzyme complex. attachment is based on interaction between boronic acid salicyl hydroxamate, thus allowing macromolecular assembly respond pH 5.0 7.4 highly reversible fashion. Catalytic...
The reaction of bis-sulfone conjugation reagents with disulfide bonds allows the site-specific modification various peptides and proteins. Herein, we present intracellular disintegration bis-sulfide containing somatostatin bioconjugates under controlled, tumor-relevant glutathione levels. GSH responsive release is demonstrated, which offers high potential for designing tumor therapeutics.
Summary Dopamine offers the possibility to build polymer films on any substrates via an oxidative process. This redox process cannot only be used for self‐oxidation in air, but also electrochemical deposition using cyclic voltammetry. Electropolymerization provides opportunity customize film thicknesses surfaces detectable by X‐ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The topography nanomechanical properties of have been characterized liquid AFM....
Conventional grafting-to approaches to DNA–polymer conjugates are often limited by low reaction yields due the sterically hindered coupling of a presynthesized polymer DNA. The grafting-from strategy, in contrast, allows one directly graft polymers from an initiator that is covalently attached Herein, we report blue-light-mediated reversible addition–fragmentation chain-transfer (Photo-RAFT) polymerization two different RAFT agent-terminated DNA sequences using Eosin Y as photocatalyst...
Temporal and spatial control over polydopamine formation on the nanoscale can be achieved by installing an irradiation-sensitive polymerization system DNA origami. Precisely distributed G-quadruplex structures template serve as anchors for embedding photosensitizer protoporphyrin IX, which-upon irradiation with visible light-induces multistep oxidation of dopamine to polydopamine, producing polymeric designated areas within origami framework. The photochemical process allows exclusive layer...
Nanostructures derived from amphiphilic DNA-polymer conjugates have emerged prominently due to their rich self-assembly behavior; however, synthesis is traditionally challenging. Here, we report a novel platform technology towards nanostructures of various shapes by leveraging polymerization-induced (PISA) for polymerization single-stranded DNA (ssDNA). A "grafting from" protocol thermal RAFT ssDNA under ambient conditions was developed and utilized the functional DNA-diblock acrylates...
Nanostructure-based functions are omnipresent in nature and essential for the diversity of life. Unlike small molecules, which often inhibitors enzymes or biomimetics with established methods elucidation, we show that nanoscale structures cells complex can implicate system-level effects such as regulation energy redox homeostasis. Herein, design a platinum(II)-containing tripeptide assembles into intracellular fibrillar nanostructures upon molecular rearrangement presence endogenous H2O2....
The complex dynamics and transience of assembly pathways in living systems complicate the understanding these molecular to nanoscale processes. Current technologies are unable track events leading onset assembly, where real-time information is imperative correlate their rich biology. Using a chemically designed pro-assembling molecule, we map its transformation into nanofibers fusion with endosomes form hollow fiber clusters. Tracked by phasor-fluorescence lifetime imaging (phasor-FLIM)...
The effects of hydration on two types structural rearrangements, physical aging and crystallization, poly(lactic acid) (PLA) have been characterized. Although the water absorbed is extremely small, order 0.5% by weight, we found a significant increase in both magnitude kinetics enthalpic relaxation near glass transition temperature (Tg). at least 2 times speed as compared to dry samples. In addition, also that crystallization can be increased significantly when PLA hydrated. initial rate 80...
Abstract Neurological disorders are undoubtedly among the most alarming diseases humans might face. In treatment of neurological disorders, blood‐brain barrier (BBB) is a challenging obstacle preventing drug penetration into brain. Advances in dendrimer chemistry for central nervous system (CNS) treatments presented here. A poly(amido)amine (PAMAM) bioconjugate with streptavidin adapter attachment dendrons or any biotinylated constructed. vitro studies on porcine murine models and vivo mouse...
Dynamic covalent chemistry (DCvC) has emerged as a versatile synthetic tool for devising stable, stimuli-responsive linkers or conjugates. The interplay of binding affinity, association and dissociation constants exhibits strong influence on the selectivity reaction, conversion rate, well stability in aqueous solutions. Nevertheless, dynamic interactions often exhibit fast events vice versa, affecting their rates stabilities. To overcome limitation linker design, we reported herein dual...
Drug safety and efficacy due to premature release into the bloodstream poor biodistribution remains a problem despite seminal advances in this area. To circumvent these limitations, we report drug cyclization based on dynamic covalent linkages devise dual lock for small-molecule anticancer drug, camptothecin (CPT). activity is "locked" within cyclic structure by redox responsive disulfide pH-responsive boronic acid-salicylhydroxamate turns only presence of acidic pH, reactive oxygen species...
ConspectusChemical programming of macromolecular structures to instill a set defined chemical properties designed behave in sequential and precise manner is characteristic vision for creating next generation nanomaterials. In this context, biopolymers such as proteins nucleic acids provide an attractive platform the integration complex design due their sequence specificity geometric definition, which allows accurate translation functionalities biological activity. Coupled with advent amino...