- Hydrocarbon exploration and reservoir analysis
- Microbial bioremediation and biosurfactants
- Geology and Paleoclimatology Research
- Petroleum Processing and Analysis
- Methane Hydrates and Related Phenomena
- Atmospheric and Environmental Gas Dynamics
- Isotope Analysis in Ecology
- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- Enhanced Oil Recovery Techniques
- Geological formations and processes
- Marine and environmental studies
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Geological Studies and Exploration
- Anaerobic Digestion and Biogas Production
- Geochemistry and Geologic Mapping
- Wastewater Treatment and Nitrogen Removal
- Toxic Organic Pollutants Impact
- Paleontology and Stratigraphy of Fossils
- Porphyrin Metabolism and Disorders
- Microbial Fuel Cells and Bioremediation
- Coal and Its By-products
- Chemical Reactions and Isotopes
- Folate and B Vitamins Research
Carl von Ossietzky Universität Oldenburg
2016-2025
GFZ Helmholtz Centre for Geosciences
2011-2022
Max Planck Institute for Marine Microbiology
1995-2012
Max Planck Society
1995-2012
Newcastle University
2011
University of Potsdam
2009-2010
Forschungszentrum Jülich
1997-2004
Ernst Ruska Centre
1997
Universität Hamburg
1989-1995
Montanuniversität Leoben
1995
In the course of our screening for dibenzo-p-dioxin-utilizing bacteria, a Sphingomonas sp. strain was isolated from enrichment cultures inoculated with water samples river Elbe. The isolate grew both biaryl ethers dibenzo-p-dioxin and dibenzofuran (DF) as sole sources carbon energy, showing doubling times about 8 5 h, respectively. Biodegradation two aromatic compounds initially proceeded after an oxygenolytic attack at angular position adjacent to ether bridge, producing...
ABSTRACT A novel type of denitrifying bacterium (strain HxN1) with the capacity to oxidize n -alkanes anaerobically nitrate as electron acceptor CO 2 formed (1-methylpentyl)succinate (MPS) during growth on -hexane only organic substrate under strict exclusion air. Identification MPS by gas chromatography-mass spectrometry was based comparison a synthetic standard. not anaerobic -hexanoate. Anaerobic [1- 13 C] or d 14 - led 1-methylpentyl side chain in one C atom deuterium atoms,...
A mesophilic sulfate-reducing enrichment culture growing anaerobically on crude oil was used as a model system to study which nutritional types of bacteria may develop original petroleum constituents in wells, tanks, and pipelines. Chemical analysis hydrocarbons during growth revealed depletion toluene o-xylene within 1 month m-xylene, o-ethyltoluene, m-ethyltoluene, m-propyltoluene, m-isopropyltoluene approximately 2 months. In anaerobic counting series, the highest numbers CFU (6 x 10(6) 8...
ABSTRACT Anaerobic degradation of the aromatic hydrocarbon ethylbenzene was studied with sulfate as electron acceptor. Enrichment cultures prepared marine sediment samples from different locations showed ethylbenzene-dependent reduction to sulfide and always contained a characteristic cell type that formed gas vesicles towards end growth. A pure culture this type, strain EbS7, isolated Guaymas Basin (Gulf California). Complete mineralization coupled demonstrated in growth experiments EbS7....
Two Pseudomonas sp. strains, capable of growth on chlorinated benzenes as the sole source carbon and energy, were isolated by selective enrichment from soil samples an industrial waste deposit. Strain PS12 grew monochlorobenzene, all three isomeric dichlorobenzenes, 1,2,4-trichlorobenzene (1,2,4-TCB). PS14 additionally used 1,2,4,5-tetrachlorobenzene (1,2,4,5-TeCB). During these compounds both strains released stoichiometric amounts chloride ions. The first steps catabolism 1,2,4-TCB...
The anaerobic biodegradation of naphthalene, an aromatic hydrocarbon in tar and petroleum, has been repeatedly observed environments but scarcely pure cultures. To further explore the relationships physiology naphthalene-degrading microorganisms, sulfate-reducing bacteria (SRB) were enriched from a Mediterranean sediment with added naphthalene. Two strains (NaphS3, NaphS6) oval cells isolated which showed naphthalene-dependent sulfate reduction. According to 16S rRNA gene sequences, both...
A Pseudomonas sp. strain, HH69, and a mixed culture, designated HH27, were isolated by selective enrichment from soil samples. The pure strain the culture grew aerobically on dibenzofuran as sole source of carbon energy. Degradation proceeded via salicylic acid which was branched into gentisic catechol pathway. Both accumulated in medium HH69. acids slowly metabolized after growth ceased. enzymes responsible for their metabolism showed relatively low activities. Besides above-mentioned...
Summary Microorganisms can degrade saturated hydrocarbons (alkanes) not only under oxic but also anoxic conditions. Three denitrifying isolates (strains HxN1, OcN1, HdN1) able to grow conditions by coupling alkane oxidation CO 2 with NO 3 ‐ reduction N were compared respect their metabolism. Strains HxN1 and which are both Betaproteobacteria , utilized n ‐alkanes from C 6 8 12 respectively. Both activate alkanes anaerobically in a fumarate‐dependent reaction yielding alkylsuccinates, as...
The Posidonia Shale in the Hils syncline North-West Germany represents a natural maturity sequence from 0.48 to 1.45% vitrinite reflectance (R0). In this study, molecular composition of acidic compounds six samples with different maturation level has been determined using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) combined electrospray ionization (ESI) negative mode. changes distribution elemental and compound classes as well double-bond equivalents (DBE) carbon...
Abstract Vitamin B12 (cobalamin, herein B12) is an essential cofactor involved in amino acid synthesis and carbon resupply to the TCA cycle for most prokaryotes, eukaryotic microorganisms, animals. Despite being required by most, produced only a minor fraction of prokaryotes therefore leads complex interaction between prototrophs auxotrophs. However, it unknown how provided In this study, 33 prototrophic alphaproteobacterial strains were grown co-culture with Thalassiosira pseudonana,...
The bacterium Sphingomonas sp. strain SS3, which utilizes diphenyl ether and its 4-fluoro, 4-chloro, (to a considerably lesser extent) 4-bromo derivatives as sole sources of carbon energy, was enriched from soil samples an industrial waste deposit. showed cometabolic activities toward all other isomeric monohalogenated ethers. During degradation in batch culture experiments, phenol catechol were produced intermediates then channeled into the 3-oxoadipate pathway. initial step follows...
The ability of the dibenzofuran- and dibenzo-p-dioxin-mineralizing bacterium Sphingomonas sp. strain RW1 (R.-M. Wittich, H. Wilkes, V. Sinnwell, W. Francke, P. Fortnagel, Appl. Environ. Microbiol. 58:1005-1010, 1992) to oxidize chlorinated derivatives dibenzofuran dibenzo-p-dioxin was analyzed. Strain degraded several mono- dichlorinated dibenzofurans dibenzo-p-dioxins, but it did not degrade more highly congeners. Most dibenzo-p-dioxins investigated in this study were corresponding...
Summary Among the dominant deltaproteobacterial sulfate‐reducing bacteria ( SRB ), members of genus D esulfobacula are not only present in (hydrocarbon‐rich) marine sediments, but occur also frequently anoxic water bodies encountered upwelling areas. Here, we 5.2 Mbp genome toluolica Tol2 , which is first an aromatic compound‐degrading, . The has apparently been shaped by viral attacks (e.g. CRISPRs ) and its high plasticity reflected 163 detected genes related to transposases integrases, a...