A5039240064- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- Gene Regulatory Network Analysis
- Plant Reproductive Biology
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Telomeres, Telomerase, and Senescence
- Sympathectomy and Hyperhidrosis Treatments
- Neonatal Respiratory Health Research
- Plant and Biological Electrophysiology Studies
- Themes in Literature Analysis
- Cholesterol and Lipid Metabolism
- RNA regulation and disease
- Plant nutrient uptake and metabolism
- Emotional Intelligence and Performance
- 3D Printing in Biomedical Research
- Lymphatic System and Diseases
- Pain Management and Placebo Effect
- Gene expression and cancer classification
- Scientific Computing and Data Management
Johns Hopkins Medicine
2017-2025
Johns Hopkins University
2017-2025
University of Pennsylvania
2024
University of Bern
2024
University of Washington
2020
Dankook University
2016
Abstract Pancreatic ductal adenocarcinoma (PDAC) is believed to arise from the accumulation of a series somatic mutations and also frequently associated with pancreatic intraepithelial neoplasia (PanIN) lesions. However, there still debate as whether cell type-of-origin PanINs PDACs in humans acinar or ductal. As type identity maintained epigenetically, DNA methylation changes during can provide compelling perspective examine this question. Here, we performed laser-capture microdissection on...
The anterior cingulate cortex plays a pivotal role in the cognitive and affective aspects of pain perception. Both endogenous exogenous opioid signaling within mitigate cortical nociception, reducing unpleasantness. However, specific functional molecular identities cells mediating analgesia remain elusive. Given complexity as sensory emotional experience, richness ethological pain-related behaviors, we developed standardized, deep-learning platform for deconstructing behavior dynamics...
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Abstract T cells are important in the pathogenesis of acute kidney injury (AKI), and TCR + CD4 - CD8 (double negative-DN) that have regulatory properties. However, there is limited information on DN compared to traditional cells. To elucidate molecular signature spatial dynamics during AKI, we performed single-cell RNA sequencing (scRNA-seq) sorted murine DN, , combined with transcriptomic profiling normal post AKI mouse kidneys. scRNA-seq revealed distinct transcriptional profiles for...
A critical area of recent cancer research is the emergence transition states between normal and that exhibit increased cell plasticity which underlies tumor heterogeneity. Pancreatic ductal adenocarcinoma (PDAC) can arise from combination a state termed acinar-to-ductal metaplasia (ADM) gain-of-function mutation in proto-oncogene KRAS. During ADM, digestive enzyme-producing acinar cells acquire transient epithelium-like phenotype while maintaining their geographical organization. One route...
Optimization of cell engineering protocols requires standard, comprehensive quality metrics. We previously developed CellNet, a computational tool to quantitatively assess the transcriptional fidelity engineered cells compared with their natural counterparts, based on bulk-derived expression profiles. However, this platform and others were limited in ability compare data from different sources, no current makes it easy new existing state-of-the-art standardized manner. Here, we utilized our...
Stomata are epidermal valves that facilitate gas exchange between plants and their environment. Stomatal patterning is regulated by EPIDERMAL PATTERING FACTOR (EPF)-family of secreted peptides: EPF1 enforcing stomatal spacing, whereas EPF-LIKE9, also known as Stomagen, promoting development. It remains unknown, however, how far these signaling peptides act. Utilizing Cre-lox recombination-based mosaic sectors overexpress either or Stomagen in Arabidopsis cotyledons, we reveal a range within...
Tissue engineering strategies that combine human pluripotent stem cell-derived myogenic progenitors (hPDMs) with advanced biomaterials provide promising tools for 3D skeletal muscle grafts to model tissue development in vitro and promote regeneration vivo. We recently demonstrated (i) the potential obtaining large numbers of hPDMs using a combination two small molecules without overexpression transgenes (ii) application electrospun fibrin microfiber bundles functional restoration following...
BACKGROUND: Cerebral intraventricular hemorrhage (IVH) is a major cause of neurodevelopmental impairment in preterm infants.IVH characterized by vessel rupture and rapid accumulation blood within the ventricles.Subsequent hemolysis leads to release extracellular hemoglobin (Hb) into cerebrospinal fl uid (CSF).Hb its metabolites initiate cytotoxic, oxidative, proinfl ammatory, apoptotic pathways resulting tissue damage.Scavenging Hb, using haptoglobin (Hp), may therefore constitute potential...