A5057284434- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Molecular Junctions and Nanostructures
- Analytical Chemistry and Sensors
- Nanoparticle-Based Drug Delivery
- Photochromic and Fluorescence Chemistry
- Nanoplatforms for cancer theranostics
- Molecular Sensors and Ion Detection
- Diamond and Carbon-based Materials Research
- Nitric Oxide and Endothelin Effects
- Carbon Nanotubes in Composites
- RNA and protein synthesis mechanisms
- Chemistry and Chemical Engineering
- Nanocluster Synthesis and Applications
- Hemoglobin structure and function
- DNA and Biological Computing
- Porphyrin and Phthalocyanine Chemistry
- Lipid Membrane Structure and Behavior
- Supramolecular Self-Assembly in Materials
City University of Hong Kong
2015-2024
Chinese University of Hong Kong
2023-2024
City University of Hong Kong, Shenzhen Research Institute
2015-2024
Kowloon Hospital
2018-2021
We have demonstrated that the subcellular targeting properties of indole-based cyanines can be tuned by functional substituent attached onto indole moiety in which first example a highly RNA-selective and two-photon active fluorescent light-up probe for high contrast brightness TPEF images rRNA nucleolus live cells has been developed. It is important to find this cyanine binds much stronger toward RNA than DNA buffer solution as well selectively stains targets nucleolus. Remarkably, (Φσmax)...
The chair-type G-quadruplex structure formed by human telomeric variant DNA.
Abstract We demonstrate the use of two different wavelength ranges excitation light as inputs to remotely trigger responses self‐assembled DNA devices (D‐OR). As an important feature this device, dependence readout fluorescent signals on external inputs, UV for 1 min and/or near infrared irradiation (NIR) at 800 nm fs laser pulses, can mimic function signal communication in OR logic gates. Their operations could be reset easily its initial state. Furthermore, these exhibit efficient cellular...
Because of the chemical simplicity α-l-threose nucleic acid (TNA) and its ability to exchange genetic information between itself RNA, it has attracted significant interest as RNA ancestor. We herein explore biological properties evaluate potency sequence-designed TNA polymers suppress gene expression in living environments. found that sequence-specific macromolecules exhibit strong affinity specificity toward complementary targets, are highly biocompatible nontoxic a cell system, readily...
Senescent cells have become an important therapeutic target for many age-related dysfunctions and diseases. We report herein a novel nanophotosensitizing system that is responsive to the senescence-associated β-galactosidase (β-gal) selective detection elimination of these cells. It involves dimeric zinc(II) phthalocyanine linked β-galactose unit via self-immolative linker. This compound can self-assemble in aqueous media, forming stable nanoscale particles which units are stacked...
The first example of mitochondrial delivery the anticancer drug doxorubicin (Dox) is presented by lipid‐functionalized DNA nanocages (LNCs). Dox localized in mitochondria induces significant cytotoxicity and cellular apoptosis MCF‐7 compared with lysosomes. These results suggest that LNC has potential to be an outstanding tool treatment specific organelle‐related diseases such as cancers.
We design and synthesize a sequence-defined α-l-threose nucleic acid (TNA) polymer, which is complementary to certain nucleotide sites of target anti-apoptotic proteins, BcL-2 involving in development progression tumors. Compared scramble TNA, anti-BcL-2 TNA significantly suppresses mRNA protein expression cancerous cells shows antitumor activity carcinoma xenografts, resulting suppression tumor cell growth induction death. Together with good biocompatibility, very low toxicity, excellent...
The rapidly emerging DNA nanotechnology began with pioneer Seeman’s hypothesis that not only can carry genetic information but also be used as molecular organizer to create well‐designed and controllable nanomaterials for applications in materials science, nanotechnology, biology. DNA‐based self‐assembly represents a versatile system nanoscale construction due the well‐characterized conformation of its predictability formation base pairs. structural features nucleic acids form basis...
Two-photon-regulated, shape-changing DNA nanostructures are demonstrated by integrating a nanotube with two-photon photocleavable module that enables the opening of cavities tube, and becomes partially single-stranded in response to excitation under 800 nm fs laser pulses.
The development of biocompatible drug delivery vehicles for cancer therapy in the brain remains a big challenge. In this study, we designed self-assembled DNA nanocages functionalized with or without blood–brain barrier (BBB)-targeting ligands, d and investigated their penetration across BBB. Our were not cytotoxic they substantially taken up capillary endothelial cells Uppsala 87 malignant glioma (U-87 MG) cells. We found that ligand modification is essential system as ligand-free (LF-NCs)...
A novel biocompatible and macrophage cell-membrane permeable carbazole-based cyanine probe for ROS detection cellular protection is reported.
A protein-derived nanodiamond-based nanoprobe with targeting properties is established for brain tumor imaging.
Abstract We demonstrate the use of two different wavelength ranges excitation light as inputs to remotely trigger responses self‐assembled DNA devices (D‐OR). As an important feature this device, dependence readout fluorescent signals on external inputs, UV for 1 min and/or near infrared irradiation (NIR) at 800 nm fs laser pulses, can mimic function signal communication in OR logic gates. Their operations could be reset easily its initial state. Furthermore, these exhibit efficient cellular...
Abstract We demonstrated a strategy to reversibly extend and contract 3D DNA nanocages based on G‐rich strands as scaffolds in the presence of K + or chelating agents. The contraction extension nanocage would be regulated by formation deformation G‐quadruplex ions agents, respectively. Compared single telomeric strands, self‐assembled integrated with three HTLs act horseradish peroxidase mimicking DNAzymes for colorimetric detection monitoring cholesterol high stability toward nuclease blood...
Combination therapy has been proven as an effective strategy for cancer treatment. To this end, we report herein a self-assembled nucleic acid-based complex dual photodynamic and antisense therapy. It contains nucleolin-targeting As1411-based G-quadruplex platform, partially hybridized oligonucleotide 4625, which can inhibit the antiapoptotic protein B cell lymphoma-xL inducing apoptotic death, zinc(II) phthalocyanine (ZnPc)-based photosensitizer held by noncovalent interactions. Through...