A5077965831- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Stress Responses and Cortisol
- Neurogenesis and neuroplasticity mechanisms
- Virus-based gene therapy research
- Neuroendocrine regulation and behavior
- RNA Interference and Gene Delivery
- Aluminum Alloys Composites Properties
- Mitochondrial Function and Pathology
- Heat shock proteins research
- Invertebrate Immune Response Mechanisms
- Animal Genetics and Reproduction
- Birth, Development, and Health
- Metallurgy and Material Forming
- Nerve injury and regeneration
- RNA Research and Splicing
- Neuropeptides and Animal Physiology
- Nuclear Structure and Function
- Fungal and yeast genetics research
- Transgenic Plants and Applications
- Microstructure and mechanical properties
- Reproductive System and Pregnancy
- Tryptophan and brain disorders
BOKU University
2018-2024
Austrian Centre of Industrial Biotechnology (Austria)
2018-2021
Inje University
2016-2017
Colorado State University
2000
Despite great efforts to control and modify gene expression of Chinese Hamster Ovary (CHO) cells by conventional genetic engineering approaches, i.e. overexpression or knockdown/-out, subclonal variation, induced unknown regulatory effects as well stress are still a major hurdle for efficient cell line unequivocal characterization function. The use epigenetic modulators - key players in CHO clonal heterogeneity has only been marginally addressed so far. Here, we present the application an...
For the industrial production of recombinant proteins in mammalian cell lines, a high rate gene expression is desired. Therefore, strong viral promoters are commonly used. However, these have several drawbacks as they override cellular responses, not integrated into network, and thus can induce stress potentially epigenetic silencing. Endogenous advantage better response to state lower level by uncontrolled overexpression transgene. Such fine-tuning typically achieved endogenous enhancers...
With the emergence of new CRISPR/dCas9 tools that enable site specific modulation DNA methylation and histone modifications, more detailed investigations contribution epigenetic regulation to precise phenotype cells in culture, including recombinant production subclones, is now possible. These also allow a wide range applications metabolic engineering once impact such modifications on chromatin state available. In this study, enhanced were targeted viral promoter (CMV), an endogenous...
Lower cultivation temperature dramatically affects cell growth and cellular productivity in Chinese hamster ovary cells (CHO) is often used industrial applications with the aim to enhance productivity. Cold-inducible proteins whose activity induced at lower play an important role understanding mechanisms of cold-induced changes gene expression. One these increased transcription specific target genes controlled by sequence elements cold-inducible promoters. This study provides a list...
Chinese hamster ovary (CHO) cells are the most widely used host for expression of therapeutic proteins. Recently, significant progress has been made due to advances in genome sequence and annotation quality unravel black box CHO. Nevertheless, many cases link between genotype phenotype context suspension cultivated production cell lines is still not fully understood. While frameshift approaches targeting coding genes frequently used, non-coding regions have received less attention with...
Phosph oenolpyruvate carboxykinase (PEPCK) is a key regulatory enzyme of renal gluconeogenesis. The 3′-nontranslated region the PEPCK mRNA contains an instability element that facilitates its rapid turnover and contributes to regulation gene expression. Such processes are mediated by specific protein-binding elements. Thus RNA gel shift analysis was used identify proteins in rat cortical cytosolic extracts bind mRNA. Deletion constructs were then map binding interactions two adjacent...
Experiment 2. FSL and FRL animals received single injections of CBD (10 or 30 mg/Kg, ip), ketamine ip) vehicle were submitted to the forced swimming test (FST) 1h later.Results: 1. Acute treatment with (30 mg/Kg) immediately after pretest, but not imipramine, significantly reduced number escape failures during test, an antidepressant-like effect similar chronic imipramine (ANOVA, p<0.05).Experiment Single injection immobility in animals, similarly p<0.05). Conclusion:Acute is able induce...