A5056572686- Cellular transport and secretion
- Signaling Pathways in Disease
- Calcium signaling and nucleotide metabolism
- Pancreatic function and diabetes
- PI3K/AKT/mTOR signaling in cancer
- Biochemical and Molecular Research
- Fungal Plant Pathogen Control
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- Ubiquitin and proteasome pathways
- Erythrocyte Function and Pathophysiology
- Lysosomal Storage Disorders Research
- Chronic Myeloid Leukemia Treatments
University of British Columbia
2022-2025
University of Victoria
2022-2024
The lipid kinase phosphatidylinositol 4 III α (PI4KIIIα/PI4KA) is a master regulator of the composition and asymmetry plasma membrane. PI4KA exists primarily in heterotrimeric complex with its regulatory proteins TTC7 FAM126. Fundamental to activity targeted recruitment membrane by lipidated EFR3A EFR3B. Here, we report cryogenic electron microscopy structure C terminus bound PI4KA-TTC7B-FAM126A complex, extensive validation using both hydrogen deuterium exchange mass spectrometry,...
The lipid kinase phosphatidylinositol 4 III alpha (PI4KIIIa/PI4KA) is a master regulator of the composition and asymmetry plasma membrane. PI4KA exists primarily in heterotrimeric complex with its regulatory proteins TTC7 FAM126. Fundamental to activity targeted recruitment membrane by lipidated EFR3A EFR3B. Here, we report cryo-EM structure C-terminus bound PI4KA-TTC7B-FAM126A complex, extensive validation using both hydrogen deuterium exchange mass spectrometry (HDX-MS), mutational...
The formation of complexes between Rab11 and its effectors regulates multiple aspects membrane trafficking, including recycling ciliogenesis. WD repeat–containing protein 44 (WDR44) is a structurally uncharacterized effector that ciliogenesis by competing with prociliogenesis factors for binding. Here, we present detailed biochemical biophysical characterization the WDR44–Rab11 complex define specific residues mediating Using AlphaFold2 modeling hydrogen/deuterium exchange mass spectrometry,...
Abstract Phosphatidylinositol 4-kinase alpha (PI4KA) maintains the PI4P and phosphatidylserine pools of plasma membrane. A key regulator PI4KA is its association into a complex with TTC7 FAM126 proteins. This can be regulated by CNAβ1 isoform phosphatase Calcineurin. We previously identified that directly binds to FAM126A. Here, we report cryo-EM structure truncated bound Calcineurin, revealing direct Calcineurin interaction PI4KA. Additional HDX-MS computational analysis show forms an...
Abstract The protein kinase Akt is a master regulator of pro-growth signalling in the cell. activated through its targeted recruitment to phosphoinositides, leading disruption autoinhibitory interface between and pleckstrin homology (PH) domains. Hyper activation common oncogenic transformation, with multiple activating mutants identified Akt. This has led development potent selective ATP-competitive allosteric inhibitors for Paradoxically, some cause hyperphosphorylation Here, using...