Application of liquid microbial inoculants on legume seeds is a sustainable agricultural practice that can improve plant nutrient uptake and increase crop productivity. Inoculants should provide long-term survival rhizobia in the final product after application, to seeds. Ten different medium formulations were examined (yeast mannitol broth with addition agar, sodium-alginate, calcium chloride, glycerol or ferric chloride combinations thereof) for efficient nitrogen-fixing rhizobium,...

This research aimed to isolate and characterize nonrhizobial endophytic bacteria from root nodules of Medicago sativa L. Lotus corniculatus with plant growth-promoting characteristics test its activity in a pot experiment acid soil.Out 44 isolates, the majority exhibited indole-3-acetic (IAA) production; 29 produced siderophores, few isolates performed phosphate solubilization and/or lytic enzymes, while 30% showed notable antifungal activity. The most promising strains were identified as...

In pot experiment, one isolate Knj from a Serbian soil, four strains of Bradyrhizobium japonicum and three spp. were examined for the effect on adzuki bean nodulation effectiveness in symbiotic N2 fixation. All tested produced root nodules bean. Strains B. showed high potential fixation, particularly 525 542. resulted 65-71% shoot dry weight 99-138% total N content uninoculated control with full (100%). No significant difference was found between plants inoculated without (40-42 42% weight,...

The potato cyst nematode (PCN) Globodera rostochiensis is a plant parasite of classified into group quarantine organisms causing high economic losses worldwide. Due to the long persistence in soil, cysts harbor numerous bacteria whose presence can lead death and population decline. G. found two fields were used as source bacteria. universal procedure was applied extract DNA from which then sequenced with 16S primers. aims study identify bacterial microbiota associated PCN populations infer...

In addition to true cereals (Poaceae) and legumes (Fabaceae), which are most commonly used as cover crops, plant species from the mustard family (Brassicaceae) increasingly due their biological agronomic characteristics. Crucifers grow fast, develop high biomass, achieve great land coverage (> 80%) possess nutrient uptake. These reasons that use crops has a beneficial effect on soil fertility, erosion prevention, weed suppression groundwater quality protection. Majority of crucifers contain...

Agricultural by-products are generated in large amounts various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction value-added compounds and production enzymes could reduce both problems its costs cellulase production. Hitherto, there have been no reported studies concerning utilization residues (after polyphenols nicotine, fermentation pre-treatment by Streptomyces fulvissimus CKS7) (CMCase ?vicelase) Paenibacillus chitinolyticus CKS1. The...

The occurrence of microorganisms and their relationships with total available heavy metal concentrations (Cu, Zn, Pb, Cr, Ni, Cd, Hg, As), as well main soil chemical characteristics (pH, P, K, Ca, N tot , C org ) were evaluated in naturally metalliferous serpentine soils non-serpentine elevated Ni Cr concentrations.Significant negative correlations detected between ammonifiers (r = -0.545,r -0.371),oligonitrofiles -0.478,r -0.458),total number -0.363,r -0.393),and Cr.The actynomicetes...

Soil chemical properties and total heavy metal concentrations (As, Cd, Cr, Cu, Ni, Pb, Zn, Fe Mn) their relationships with some soil microbiological characteristics (such as respiration numbers of specific groups microorganisms) were evaluated in agricultural soils southern Serbia. In 42% samples, slightly elevated As, Ni and/or Pb detected, the highest values 43.4, 64.4 184.1 mg/kg, respectively. No significant differences observed between samples metals below maximum allowed (MAC). There...

The study evaluates the effect of liming materials application in combination with NPK fertilizer and borax on macronutrient contents (nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) magnesium (Mg)), an extremely acid soil raspberry leaves fruits during a two-year period. Liming increased pH, N mineral content, P, Ca Mg while K content either (dolomite application), or decreased (lime application). P after significantly, but remained below optimal values. Some treatments lime...

Common birdsfoot trefoil (Lotus corniculatus L.) is a perennial forage legume tolerant to adverse environmental conditions such as low soil pH, poor drainage, various insects and plant diseases. Owing its ability establish symbiosis with nitrogen-fixing bacteria, rhizobia, thrives on soils of fertility used in grass mixtures enrich the nitrogen. The aim work was investigate presence rhizobia that nodulate Serbia perform isolation evaluation their nitrogen fixation potential. abundance 28...

A prerequisite for the improvement of birdsfoot trefoilproduction is productionof sufficient quantities good quality seeds. The aim study was to analyzethe impact seed inoculation with mesorhizobial bacteria on yield and yieldcomponents trefoil (Lotus corniculatus L. ). In experimentbirdsfoot cultivars Zora, K-37 Rocco, two strains Mesorhizobiumloti were used. trial carried out in spring 2012 a private estate thevillage Ivanovci near Ljig (Serbia) set up brown forest soil type,using...

Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion growth. The most comprehensively studied bacteria with these belong to Bacillus genus. In this study, seven isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics isolates, such as production indole-3aceticacid, siderophores and hydrogen cyanide tested. induction wheat (Triticum aestivum) barley (Hordeum jubatum) seed...

The study of five forage grasses (Lolium multiflorum, Festuca rubra, arundinacea, Arrhenatherum elatius and Dactylis glomerata) was conducted on an uncontaminated cultivated land, leached chernozem type, ?Nikola Tesla A? (TENT A) thermal power station ash deposit. concentrations of: As, Pb, Cd, Zn, Ni, Fe i Cu in grown two media were compared. Grass samples have been collected tillering stage, when they full development. During the vegetative period three replications cut at about 3-5 cm...

In the present study effects of Rhizobium inoculation and lime application on mineral composition (N, P, K, Ca, Mg, Fe, Mn, Cu, Zn, B) red clover (Trifolium pratense L.), in very acid soil were evaluated. Inoculation with leguminosarum bv. trifolii significantly increased shoot dry weight (SDW) plants (three times greater), as well N, Mn Cu contents compared to control. Application together, depending rate (3, 6 or 9 t ha-1 lime) cut, SDW significantly, but decreased Zn B plants. Regardless...