A5003357668- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Free Radicals and Antioxidants
- biodegradable polymer synthesis and properties
- RNA Interference and Gene Delivery
- Genomics, phytochemicals, and oxidative stress
- Periodontal Regeneration and Treatments
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Synthesis and biological activity
- Tissue Engineering and Regenerative Medicine
- Antioxidant Activity and Oxidative Stress
- Autophagy in Disease and Therapy
- Curcumin's Biomedical Applications
- Essential Oils and Antimicrobial Activity
- Osteoarthritis Treatment and Mechanisms
- Toxoplasma gondii Research Studies
- Nanocomposite Films for Food Packaging
- Vitamin C and Antioxidants Research
- Nanoparticle-Based Drug Delivery
- Dental Implant Techniques and Outcomes
- Viral gastroenteritis research and epidemiology
- Organoselenium and organotellurium chemistry
- Structural and Chemical Analysis of Organic and Inorganic Compounds
University of Minho
2006-2018
University of Coimbra
1983-1986
Colorectal carcinoma (CRC) is one of the most common causes cancer-related mortality. Short-chain fatty acids secreted by dietary propionibacteria from intestine, such as acetate, induce apoptosis in CRC cells and may therefore be relevant prevention therapy. We previously reported that acetic acid-induced Saccharomyces cerevisiae involves partial vacuole permeabilization release Pep4p, yeast cathepsin D (CatD), which has a protective role this process. In cancer cells, lysosomes have...
Starch-based biomaterials and scaffolds have been proposed for several biomedical applications. In the present work new based on a 50/50 (wt%) blend of corn starch/ethylene-vinyl alcohol (SEVA-C) were studied. These processed by melt-based technology, which has used before with other starch-based materials but never SEVA-C. Scanning electron microscopy (SEM) observation showed that developed porous structures 60% pore size between 200 900 μm reasonable degree interconnectivity. Moreover,...
Abstract Our purpose was to evaluate the in vivo endosseous response three starch‐based scaffolds implanted rats ( n = 54). We scaffold groups; a 50/50 (wt %) blend of corn starch and ethylene‐vinyl alcohol (SEVA‐C), same composition coated with biomimetic calcium phosphate (Ca‐P) layer (SEVA‐C/CaP), cellulose acetate (SCA), all produced by extrusion blowing agents, into distal femurs proximal epiphyseal plate, for 1, 3, or 6 weeks. results showed that at 1 week considerable reparative bone...
Two Ca2+ transport systems were investigated in plasma membrane vesicles isolated from sheep brain cortex synaptosomes by hypotonic lysis and partial purification. Synaptic loaded with Na+ (Na+i) accumulate exchange for Na+, provided that a gradient (in leads to out) is present. Agents dissipate the (monensin) prevent Na+/Ca2+ completely. accumulated can be released A 23187, indicating intravesicularly. In absence of any (K+i-loaded vesicles), also owing ATP hydrolysis. Monovalent cations...
Curcumin is a natural polyphenolic compound isolated from turmeric (Curcuma longa) with well-demonstrated neuroprotective and anticancer activities. Although curcumin safe even at high doses in humans, it exhibits poor bioavailability, mainly due to absorption, fast metabolism, rapid systemic elimination. To overcome these issues, several approaches, such as nanoparticle-mediated targeted delivery, have been undertaken different degrees of success. The present study was conducted compare the...
In a previous work, we described the use of starch-based microparticles as vehicles for controlled release corticosteroids. The goal present work is to evaluate potential these incorporate and platelet-derived growth factor (PDGF). loading efficiency profile were evaluated, PDGF was incorporated into released from matrix microparticles. shows rapid in first 24 h, after which there slow but constant up 8 weeks. maintenance biological activity incorporation evaluated by its mitogenic effect...
Abstract The aim of the development composite materials is to combine most desired properties two or more materials. In this work, biodegradable character, good controlled‐release properties, and natural origin starch‐based biomaterials are combined with bioactive bone‐bonding glass (BG). Novel, starch–BG microparticles were synthesized starting from a blend starch polylactic acid (50%/50% wt) BG 45S5 powder using simple emulsion method. Morphological chemical characterization showed that...
Abstract: The binding of [ 3 H]nimodipine to purified synaptic plasma membranes (SPM) isolated from sheep brain cortex was characterized, and the effects nimodipine, nifedipine, (+)‐verapamil on were compared 45 Ca 2+ translocation under conditions that separate fluxes through channels uptake via Na + /Ca exchange. H]Nimodipine labels a single class sites in SPM, with K D 0.64 ± 0.1 n M , B max 161 27 fmol.mg ‐1 protein, Hill slope 1.07, at 25°C. Competition SPM unlabelled channel blockers...
Abstract We previously described the synthesis of starch‐based microparticles that were shown to be bioactive (when combined with Bioactive Glass 45S5) and noncytotoxic. To further assess their potential for biomedical applications such as controlled release, three corticosteroids a similar basic structure—dexamethasone (DEX), 16α‐methylprednisonole (MP), 16α‐methylprednisolone acetate (MPA)—were used models entrapment release agents. DEX, MP, MPA entrapped into at 10% wt/wt polymer loading...
Reactive oxygen (ROS) and nitrogen (RNS) species are known to be involved in many degenerative diseases. This study reports four new compounds from organic synthesis, identified as FMA4, FMA7, FMA762 FMA796, which differ mainly by the number of hydroxyl groups within their phenolic unit. Their potential role antioxidants was evaluated PC12 cells assessing protection against oxidative nitrosative insults. The compounds, particularly were able protect lipid peroxidation intracellular ROS/RNS...