A5014687627- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Genomic variations and chromosomal abnormalities
- Fractal and DNA sequence analysis
- Child Nutrition and Water Access
- Parasite Biology and Host Interactions
- Helminth infection and control
- Genetic Mapping and Diversity in Plants and Animals
- Global Maternal and Child Health
- Lysosomal Storage Disorders Research
- Renal Diseases and Glomerulopathies
- Genetic and phenotypic traits in livestock
- Vector-borne infectious diseases
- Eosinophilic Disorders and Syndromes
- Genomics and Phylogenetic Studies
- Chronic Lymphocytic Leukemia Research
- Hemoglobinopathies and Related Disorders
- Bioinformatics and Genomic Networks
- Genetic Associations and Epidemiology
- Insect Pest Control Strategies
- Evolution and Genetic Dynamics
- Genomics and Rare Diseases
University of Liverpool
2014-2024
Authorised Association Consortium
2024
Makerere University
2017-2021
University of Glasgow
2017
Wellcome Centre for Molecular Parasitology
2017
Wellcome Trust
2017
University of London
2009
London School of Hygiene & Tropical Medicine
2009
University of Manchester
2007
Liverpool School of Tropical Medicine
1994-2006
A nested PCR was developed to amplify the variable region of kinetoplast minicircles all Leishmania species which infect mammals. Each parasite contains approximately 10,000 DNA minicircles, are unequally distributed among 10 minicircle classes. The primers were designed bind within 120-bp conserved is common classes; remaining 600 bp each highly class but divergent between generated a strong signal from minimum 0.1 fg DNA. Restriction digests amplicons highest dilutions suggested that only...
This study presents new findings concerning the evolution of human pathogens, Trypanosoma brucei and T. cruzi , which suggest that these parasites have divergent origins fundamentally different patterns evolution. Phylogenetic analysis 18S rRNA sequences places in a clade comprising exclusively mammalian trypanosomes African origin, suggesting an evolutionary history confined to Africa. (from humans sylvatic mammals) clusters with specific Old New World bats, rangeli trypanosome species...
Reduced susceptibility to infectious disease can increase the frequency of otherwise deleterious alleles. In populations African ancestry, two apolipoprotein-L1 (APOL1) variants with a recessive kidney risk, named G1 and G2, occur at high frequency. APOL1 is trypanolytic protein that confers innate resistance most trypanosomes, but not Trypanosoma brucei rhodesiense or T.b. gambiense, which cause human trypanosomiasis. this case-control study, we test prevailing hypothesis these reduce...
Phylogenetic relationships among Trypanosoma rangeli isolates from man, wild mammals and triatomine bugs widespread geographical origin were inferred by comparison of the small subunit ribosomal gene sequences. The phylogenetic trees indicated that subgenus Herpetosoma is polyphyletic strongly supported division this group into two monophyletic lineages, one made up T. , -like allied species other consisting lewisi related taxa. Based on analysis, morphology, behaviour in vertebrate...
Editorial on the Research Topic Food safety in low-and middle-income countriesThis is first special edition food informal markets low-and-middle income countries (LMICs).Despite their important public health and socio-economic impacts, foodborne diseases have only recently gained attention of development institutes initiatives (Grace, 2023).The result growing appreciation enormous burden disease LMICs: comparable to that malaria, HIV/AIDs or tuberculosis economic cost more than 100 billion...
Journal Article An outbreak of cutaneous leishmaniasis in an Afghan refugee settlement north-west Pakistan Get access Mark Rowland, Rowland 1HealthNet International, University Town, P.O. Box 889, Peshawar, Pakistan2London School Hygiene and Tropical Medicine, London WC1E 7HT, UK Address for correspondence: Disease Control Vector Biology Unit, Keppel Street, UK; fax +44 (0) 171 580 9075. m.rowland@lshtm.ac.uk Search other works by this author on: Oxford Academic PubMed Google Scholar Arif...
SSU ribosomal sequences of trypanosomes from Brazilian cattle and water buffalo were used to infer phylogenetic relationships between non-pathogenic T. theileri allied species parasitic in artiodactyls. distinct geographical regions Brazil other countries tightly clustered into the 'clade theileri' distant 'T. brucei clade' pathogenic parasites artiodactyls, also mammals. The existence this monophyletic assemblage (T. clade) composed only by isolates artiodactyl justifies continued...
DNA was isolated from 92 Giemsa-stained smears of lesions suspected cases cutaneous leishmaniasis and used for PCR-based diagnosis Leishmania infection. Each smear had been examined under a light microscope at ∈1000 scored amastigote numbers. Although the stored up to 4 years, all microscopy-positive slides were also positive by PCR four 14 that negative microscopy (although clinically consistent with leishmaniasis) PCR-positive. investigations therefore appear offer an effective method...
Isoenzyme-based studies have identified 3 taxa/species/'phylogenetic complexes' as agents of visceral leishmaniasis in Sudan: L. donovani, infantum and "L. archibaldi". However, these observations remain controversial. A new chitinase gene phylogeny was constructed which stocks all putative species isolated Sudan formed a monophyletic clade. In order to construct more robust classification the donovani complex, panel 16 microsatellite markers used describe 39 species. All complex" from were...
Abstract To investigate the relationship of cutaneous leishmaniasis isolates from Sri Lanka to known species, we performed DNA sequencing and microsatellite analyses. We identified Leishmania donovani as agent showed that these parasites are closely related those causing visceral in Indian subcontinent.
African bovine trypanosomiasis caused by Trypanosoma sp., is a major constraint on cattle productivity in sub-Saharan Africa. Some Bos taurus breeds are highly tolerant of infection, but the potentially more productive indicus zebu much susceptible. Zebu well adapted for plowing and haulage, increasing their tolerance could have impact crop cultivation as dairy beef production. We used three strategies to obtain short lists candidate genes within QTL that were previously shown regulate...
Based on phylogenetic analysis of 18S rRNA sequences and clade taxon composition, this paper adopts a biogeographical approach to understanding the evolutionary relationships human primate infective trypanosomes, Trypanosoma cruzi, T. brucei, rangeli cyclops. Results indicate that these parasites have divergent origins fundamentally different patterns evolution. cruzi is placed in with trypanosomes specific bats kangaroo. The predominantly South American Australian within suggest an ancient...
Trypanosoma congolense are extracellular protozoan parasites of the blood stream artiodactyls and one main constraints on cattle production in Africa. In cattle, anaemia is key feature disease persists after parasitaemia has declined to low or undetectable levels, but treatment clear usually resolves anaemia.The progress infection was followed three mouse strains. Anaemia developed rapidly all strains until peak first wave parasitaemia. This by a second phase, characterized slower severe...
ABSTRACT Human African trypanosomiasis is caused by two subspecies of Trypanosoma brucei . rhodesiense found in East Africa and frequently causes acute disease, while gambiense West associated with chronic disease. Samples taken from a single focus Ugandan outbreak T b. the 1980s were either or We sequenced whole genomes these isolates, which showed that they are genetically distinct each other. Analysis nucleotide polymorphism markers panel 31 isolates plus 32 controls revealed mixture...
Lutzomyia longipalpis, a sibling complex, is the main vector of Leishmania chagasi/infantum. Discriminating between siblings important as they may differ in vectorial capacity. longipalpis populations display distinct male sex pheromone chemotypes. We investigated phylogeographic pattern variation at microsatellite loci from 11 Brazil and Venezuela related to their pheromone. Temporal genetic differentiation was mostly not significant same site. Spatial was, however, strong, although there...
It is increasingly common to combine Microarray and Quantitative Trait Loci data aid the search for candidate genes responsible phenotypic variation. Workflows provide a means of systematically processing these large datasets also represent framework re-use explicit declaration experimental methods. In this article, we highlight issues facing manual analysis microarray QTL discovery underlying complex phenotypes. We show how automated approaches systematic investigate genotype-phenotype...
A multilocus microsatellite typing (MLMT) approach based on the analysis of 15 independent loci has been developed for discrimination strains belonging to different Viannia species. Thirteen were isolated de novo from microsatellite-enriched libraries both Leishmania braziliensis and L. guyanensis. Two previously identified markers, AC01 AC16, modified added our marker set. Markers designed contain simple dinucleotide repeats flanked by minimal possible number nucleotides in order allow...
Abstract African trypanosomes ( Trypanosoma ) are vector-borne haemoparasites that survive in the vertebrate bloodstream through antigenic variation of their Variant Surface Glycoprotein (VSG). Recombination, or rather segmented gene conversion, is fundamental brucei for both VSG switching and generating diversity during infections. vivax a related, livestock pathogen whose lack structures facilitate conversion T. mechanisms underlying its poorly understood. Here we show species-wide...