Sofosbuvir and ledipasvir are the first drugs in a combination pill to treat chronic hepatitis C virus. Simple, sensitive, rapid spectrophotometric methods presented for determination of sofosbuvir their combined dosage form. These were based on direct measurement at 333 nm (due lack interference sofosbuvir) over concentration range 4.0-14.0 µg/mL, with mean recovery 100.78 ± 0.64%. was determined, without prior separation, by third-derivative values 281 nm; derivative ratio 265.8 utilizing...

Montelukast is a leukotriene receptor antagonist for treatment of asthma, gliclazide an oral hypoglycemic antidiabetic agent, and nifedipine calcium channel blocker used angina pectoris hypertension. These drugs may be prescribed to patients suffering from these chronic diseases. A survey the literature reveals that there no reported method simultaneous determination montelukast, gliclazide, in pharmaceutical preparations or biological fluids.A simple, sensitive, rapid quantification human...

Two simple, rapid and precise reversed phase liquid chromatographic methods have been developed validated for the simultaneous determination of miconazole nitrate in two binary mixtures, with hydrocortisone acetate (Mixture 1) mometasone furoate 2). For separation was carried out on a C18 column. mixture 1, mobile consisting 2.22 mM sodium dihydrogen phosphate (Triethylamine 0.2%):acetonitrile (45:55, v : ) at flow rate 0.9 mL/min used ambient temperature. Quantitative achieved UV detection...

Purpose: A new sensitive sensor was fabricated for the determination of ivabradine hydrochloride (IH) based on modification with multiwalled carbon nanotubes using sodium dodecyl sulfate as micellar medium to increase sensitivity. Methods: The electrochemical behavior IH studied in Britton-Robinson buffer (pH: 2.0-11.0) cyclic and differential pulse voltammetry. Results: voltammetric response linear over range 3.984 x 10-6-3.475 10-5 mol L-1. limits detection quantification were found be...

A simple, rapid, sensitive, and precise reversed-phase liquid chromatographic method was developed validated for the simultaneous determination of four direct-acting antivirals, sofosbuvir (SF), ledipasvir (LD), declatasvir (DC), simeprevir (SM), in their respective pharmaceutical formulations. Effective separation achieved on an Agilent Eclipse plus C8 column (250 mm × 4.6 mm, 5 µm) at 40 °C with gradient elution using a mobile phase composed acetonitrile:phosphate buffer (pH 6.5). The...

An alkaline-forced degradation hydrolytic product of vinpocetine was prepared and characterized by 1H-NMR, FTIR spectroscopy, MS. Subsequently, a simple, selective, validated reversed-phase HPLC method developed for the simultaneous estimation folic acid in presence alkaline product. Chromatographic separation achieved using an isocratic mobile phase consisting acetonitrile-0.02 M KH2PO4 [containing 0.2% (v/v) triethylamine adjusted to pH 6 with orthophosphoric acid; (80 + 20, v/v)] at flow...

Development of novel sensors for the determination ivabradine hydrochloride using sulphonated calix-8-arene and cyclodextrin derivatives as neutral ionophores.

Risperidone (RIS) is used as an antipsychotic drug alone or with other drugs, like fluoxetine (FLX). A simple method was developed and validated for the determination of both RIS its metabolite 9-hydroxyrisperidone (9-OH-RIS), FLX, olanzapine (OLA) internal standard in rat’s plasma using UPLC-MS/MS. RIS, 9-OH-RIS, OLA were purified acetonitrile a protein precipitating agent. Separation performed on ACQUITY™ “UPLC BEH™” C18 column (50 mm × 2.1 i.d., 1.7 μ m; Waters Corp., USA). The ranges...

The photostability of gemifloxacin mesylate (GFLX) and lomefloxacin hydrochloride (LFLX) antibacterial agents in dilute aqueous solutions (bulk powder dosage form) was studied by applying the International Conference on Harmonization (ICH) recommended conditions. photodegradation processes were monitored UV-Vis spectrophotometry quantified HPLC. structures degradation products solution have been deduced from LC-MS/MS. GFLX LFLX kinetic rates also determined. process for can be described as...

Aspirin (ASP) and cilostazol (CST) are used as a combination in pharmaceutical formulations for treatment of strokes. Salicylic acid (SAL) is considered to be one the main synthesis impurities degradation product ASP. On other hand, related CST A, B, C (CST-RA, CST-RB, CST-RC), respectively. Furthermore, high efficiency less elution basic requirements high-speed chromatographic separation, so, comparative study two simple, precise, accurate reversed-phase HPLC UPLC methods was developed...

Tioconazole (TCN) (anti fungal) is commonly co-formulated with tinidazole (TND) (antiprotozoal), hydrocortisone acetate (HYD) infalmmatory) and benzyl alcohol (BZL) (preservative) in different pharmaceutical preparations. has three related impurities namely; tioconazole A, B C (TCN-RA), (TCN-RB) (TCN-RC), respectively. As the main purpose of a test for substances to control degradation limit arising during synthesis active ingredients on other hand, high efficiency less elution are basic...

The construction and electrochemical response characteristics of Poly (vinyl chloride) membrane sensors for bambuterol HCl (Bb) are described. sensing membranes incorporate ion association complexes cation phosphomolybdic acid (PMA) (sensor 1) phosphotungstic (PTA) 2) as electroactive materials. display a fast, stable near-Nernstian over relative wide concentration range (1 × 10−2 to 7×10−6, 1 6.0×10−6M), with detection limits 5×10-6, 3×10-6 sensor 1, 2, respectively pH 3.0 - 8.0. show good...

A simple, rapid, sensitive and accurate HPLC–UV detection method was developed validated for the determination of isotretinoin in human plasma. The involves protein precipitation plasma by acetonitrile using nifedipine as internal standard (IS). Chromatographic separation achieved on Agilent C18 column an acetonitrile: aqueous 0.5% glacial acetic acid (70:30, v/v) mobile phase. Isotretinoin eluent monitored at 350 nm. Inter-assay coefficient variations (CV%) over range calibration curves...

Accurate, precise, and sensitive LC-MS/MS assay method for the determination of montelukast (MO) in human plasma samples using gliclazide (GL) as internal standard was developed applied pharmacokinetics study.MO extracted by protein precipitation acetonitrile. Chromatographic separation carried out a Agilant Triple quadrupoles mass spectrometer with API source an SB- C18 (50×4.6 mm), 1.8 µm particle size column. A mobile phase consisting acetonitrile: 0.1% formic acid (84:16) delivered....

Sensitive and specific liquid-chromatography tandem mass spectrometry (UPLC-MS/MS) assay has been developed validated for simultaneous quantification of risperidone (RIS) its active metabolite 9-hydroxyrisperidone (9-OH-RIS) in rat plasma using olanzapine (OLA) as internal standard (IS). Pharmacokinetics was compared across different doses (0.3, 1.0, 6.0 mg/kg). Serial blood sample collected over a time 48 hours analyzed 9-hydroxyrisperidone. The pharmacokinetics parameters including...

Aspirin (ASP) (analgesic; anti-inflammatory; anti-platelet), clopidogrel bisulphate (CLP) (anti-platelet) and atorvastatin calcium (ATV) (anti-hyperlipidemia) are co-formulated in several pharmaceutical formulations. Salicylic acid (SAL), carboxylic metabolite related impurity A (CLP-RA) inactive R-enantiomer C (CLP-RC) considered to be the main synthesis impurities degradation products of ASP clopidogrel. On other hand, high efficiency less elution basic requirements high-speed...

Bambuterol HCl (BMT) (bronchodilator) was subjected to stress alkaline hydrolysis. BMT degradation product (BMT-DP) prepared, isolated and verified chemically using Fourier transform infrared spectroscopy (FTIR), Proton nuclear magnetic resonance (1H-NMR) as well mass spectrometry. Na benzoate (BNZ) is commonly co-formulated in oral pharmaceutical preparations a preservative. Subsequently, simple, selective valid stability indicating reversed phase high performance liquid chromatography...

Simple, sensitive, accurate and validated bioanalytical liquid chromatographic procedure coupled with fluorescence detector for estimation of valsartan in presence diclofenac as internal standard (IS) plasma has been established. The extraction IS was achieved using methyl tertiary-butyl ether: ethyl acetate (2:8) mixture the extracted samples were separated on Waters, Xterra- C18 (150 x 4.6 mm, 5 μm) column 1.0 mL/min flow rate. detection performed at excitation emission λ 255 nm 374 nm,...

The lornoxicam (LRX) and tenoxicam (TNX) drugs form a stable 1:1 inclusion complex with b-cyclodextrin (β-CD) in aqueous solution.The experimentally determined association constants (K) of LRX-β-CD TNX-β-CD are 13.4 10.3 M -1 , respectively.Quantum chemical computations simulated the preferred orientation guest molecules host.Geometry optimized results using ONIOM technique provided more in-depth insights identified structure showed that both were partially encapsulated within β-CD...

Correction for ‘Electrochemical study of ivabradine hydrochloride ion selective electrodes using different ionophores’ by Nisreen F. Abo-Talib <italic>et al.</italic>, <italic>RSC Adv.</italic>, 2015, <bold>5</bold>, 95592–95597.

Correction to: Development of an LC-Tandem Mass Spectrometry Method for the Separation Montelukast and its Application to a Pharmacokinetic Study in HumansDrug Res (Stuttg) 2014; 64(11): 617-622DOI: 10.1055/s-0034-1366986