A5100716926- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Fungal Biology and Applications
- Composting and Vermicomposting Techniques
- Viral gastroenteritis research and epidemiology
- Plant-Microbe Interactions and Immunity
- Vibrio bacteria research studies
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Microbial metabolism and enzyme function
- Echinoderm biology and ecology
- Microbial Metabolism and Applications
- Heme Oxygenase-1 and Carbon Monoxide
- Ginseng Biological Effects and Applications
- Enzyme Catalysis and Immobilization
- Nuts composition and effects
- Papaya Research and Applications
- Algal biology and biofuel production
- Seaweed-derived Bioactive Compounds
- Thermal Expansion and Ionic Conductivity
- Marine Bivalve and Aquaculture Studies
- Natural Products and Biological Research
Dalian University of Technology
2010-2025
Dalian University
2012-2025
Tsinghua University
2024
Hainan University
2011-2023
University Medical Center Hamburg-Eppendorf
2023
Universität Hamburg
2023
Zhongda Hospital Southeast University
2023
Jiangnan University
2020-2021
Ministry of Education of the People's Republic of China
2016-2020
Huazhong Agricultural University
2007
Multidrug-resistant Klebsiella pneumoniae (MRKP) has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be potential alternative for treating bacterial infections. In this study, lytic bacteriophage, phage 1513, was isolated using clinical MRKP isolate KP 1513 as the host and characterized. It produced clear plaque with halo classified Siphoviridae. had short latent period of 30 min, burst size 264 could inhibit growth in vitro dose-dependent pattern....
Summary The emergence and rapid spread of multidrug‐resistant bacteria has induced intense research for novel therapeutic approaches. In this study, the Acinetobacter baumannii bacteriophage D2 (vB_AbaP_D2) was isolated, characterized sequenced. endolysin D2, namely Abtn‐4, contains an amphipathic helix found to have activity against Gram‐negative strains. By more than 3 log units, A. were killed by Abtn‐4 (5 µM) in 2 h. absence outer membrane permeabilizers, exhibited broad antimicrobial...
Sucrose phosphorylase (SPase) can specifically catalyze transglycosylation reactions and be used to enzymatically synthesize α-D-glycosides. However, the low thermostability of SPase has been a bottleneck for its industrial application. In this study, gene from Leuconostoc mesenteroides ATCC 12,291 (LmSPase) was synthesized with optimized codons overexpressed successfully in Escherichia coli. A semi-rational design strategy that combined FireProt (a web server designing thermostable...
ABSTRACT The marine ecosystem is characterized by a rich diversity of bacterial hosts and their phages. propagation phages primarily limited ability to adsorb host cells further challenged various defense mechanisms. To fully realize the potential phage therapy in aquaculture, comprehensive understanding phage-host interactions regulation essential. In this study, we isolated novel Myoviridae phage, BX-1, capable infecting Vibrio alginolyticus AP-1, its resistant mutants. We elucidated...
Pyrroloquinoline quinone (PQQ) is a novel redox cofactor and also exists in various foods. In vivo as well vitro experimental studies have shown that PQQ functions an essential nutrient or antioxidant. Methylmercury (MeHg), highly toxic environmental pollutant, could elicit central nervous system (CNS) damage. Considering the antioxidant properties of PQQ, this study was aimed to evaluate effect on MeHg-induced neurotoxicity PC12 cells. The results showed that, after pre-treatment cells with...
ABSTRACT Acinetobacter baumannii , an opportunistic pathogen, poses a significant threat in intensive care units, leading to severe nosocomial infections. The rise of multi-drug-resistant strains, particularly carbapenem-resistant A. has created formidable challenges for effective treatment. Given the prolonged development cycle and high costs associated with antibiotics, phages have garnered clinical attention as alternative combating infections caused by drug-resistant bacteria. However,...
Abstract Acinetobacter baumannii , which is resistant to multiple drugs, an opportunistic pathogen responsible for severe nosocomial infections. With no antibiotics available, phages have obtained clinical attention. However, since immunocompromised patients are often susceptible infection, the appropriate timing of administration particularly important. During this research, we a lytic phage vB_AbaM_P1 that specifically targets A. . We then assessed its potential as prophylactic treatment...
Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or pigment on performance and egg yolk color laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown hens fed one seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 9.6 ppm 0.3 carophyll red. Each diet was three replicate batteries with each battery consisting a row five cages two per cage (n = 3). 2, 180, Hyline hens, housed similarly those in an unsupplemented basal as well treatments which...
Estrogens regulate synaptic properties and influence hippocampus-related learning memory via estrogen receptors, which include the G-protein-coupled receptor 1 (GPER1). Studying mice, in GPER1 gene is dysfunctional (GPER1-KO), we here provide evidence for sex-specific roles of these processes. GPER1-KO males showed reduced anxiety elevated plus maze, whereas fear response ('freezing') was specifically increased females a contextual conditioning paradigm. In Morris water spatial consolidation...
The alga Laminaria japonica is the most economically important brown seaweed cultured in China, which used as food and aquatic animal feedstuff. However, use of L. a feedstuff Apostichopus japonicas farming not ideal because A. does produce enough enzyme activity for degrading large amount algin present japonica. In this study, semi solid fermentation employing Bacillus strain microbe was to mean degrade content strain, amyloliquefaciens WB1, isolated by virtue its ability utilize sodium...
Disposal of animal carcasses by co-composting with waste usually selected conventional carbon to nitrogen (C/N) ratio around 25:1, in which the compost is widely used throughout world. In this study, pig carcass tissue blocks were sampled for composting at a laboratory scale evaluate effect C/N on compost. The time thermophilic phase between 60 °C - 70 lower 20:1 was significantly longer than that ratio, and it only one temperature beyond lasted 2 days. Germination index T value (the final /...
Enterotoxigenic Escherichia coli (ETEC) infection is a primary cause of livestock diarrhea. Therefore, effective vaccines are needed to reduce the incidence ETEC infection.Our study aimed develop multivalent vaccine targeting major virulence factors ETEC, including enterotoxins and fimbriae.SLS (STa-LTB-STb) recombinant enterotoxin fimbriae proteins (F4, F5, F6, F18, F41) were prepared vaccine. A total 65 mice immunized subcutaneously by phosphate-buffered saline (PBS). The levels specific...
Sucrose phosphorylase (SPase) gene from Leuconostoc mesenteroides ATCC 12291 was synthesised after codon optimization, and inserted into pET-28a plasmid to generate pET-28a-spase. The recombinant strain Escherichia coli BL21 (DE3)/pET-28a-spase induced for Spase expression. protein purified characterized. specific enzyme activity of SPase 213.98 U/mg, the purification ratio 1.47-fold, recovery rate 87.80%. optimal temperature pH were identified be 45 °C 6.5 respectively, Km, Vmax kcat...