A5101724632- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Environmental remediation with nanomaterials
- Electrochemical Analysis and Applications
- Adsorption and biosorption for pollutant removal
- Chromium effects and bioremediation
- Advanced biosensing and bioanalysis techniques
- Acute Kidney Injury Research
- Ubiquitin and proteasome pathways
- Analytical Chemistry and Sensors
- Advanced Drug Delivery Systems
- Peptidase Inhibition and Analysis
- Inorganic Fluorides and Related Compounds
- Electron and X-Ray Spectroscopy Techniques
- Radioactive element chemistry and processing
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- GaN-based semiconductor devices and materials
- Synthesis of Organic Compounds
- Microbial Natural Products and Biosynthesis
- Fluorine in Organic Chemistry
- Biosensors and Analytical Detection
- Protein purification and stability
- Click Chemistry and Applications
- Enzyme Structure and Function
Army Medical University
2024
Chinese Academy of Sciences
2008-2020
Chengdu Institute of Biology
2020
Chengdu University
2020
Chinese University of Hong Kong
2014-2019
Fujian Institute of Research on the Structure of Matter
2008-2015
Institute of Semiconductors
2015
Institute of Biophysics
2014
National Synchrotron Radiation Laboratory
2010
Institute of High Energy Physics
2010
Bioremediation of Cr(VI) through reduction relies on the notion that produced Cr(III) may be precipitated or efficiently immobilized. However, recent reports suggest soluble organo-Cr(III) complexes are present in various chromate-reducing bacterial systems. This work was designed to explore factors affect immobilization Ochrobactrum anthropi system. X-ray absorption fine structure analysis cell debris clearly verified coordination occurs surfaces via chelating with carboxyl- and...
A basic understanding related to the immobilization of chromium by bacteria is essential for chromate pollutant remediation in environment. In this work, we studied Cr(VI) uptake mechanism living Ochrobactrum anthropi and influence a bacterial culture medium on Cr-immobilization process. It was found that ratio Tris-HCl buffer higher than LB medium. X-ray photoelectron spectroscopy (XPS) electron paramagnetic resonance (EPR) analysis revealed accumulated were mostly Cr(III) states. Scanning...
Intracellular reactions on nonenzymatic proteins that activate cellular signals are rarely found. We report one example here a designed peptide derivative undergoes nucleophilic reaction specifically with cytosolic PDZ protein inside cells. This led to the activation of ephrin-B reverse signaling, which subsequently inhibited SDF-1 induced neuronal chemotaxis human neuroblastoma cells and mouse cerebellar granule neurons. Our work provides direct evidence PDZ-RGS3 bridges signaling G for first time.
Specific protein-peptide interactions are prevalent in the living cells and form a tightly regulated signaling network. These interactions, many of which have structural information revealed, provide ideal templates for affinity-guided covalent bioconjugation. Here we report development set four new reactions that covalently site-specifically link nonenzymatic scaffolding domains (two PDZ two SH3 domains) their ligands through thiol-chloroacetyl SN2 reaction. Guided by three-dimensional...
A key challenge in bioconjugation is to control the site selectivity of reaction. Chemical reagents often react with proteineous chemical groups without showing preference their location or microenvironment protein; confine reaction an amino acid at a specific site, one needs distinguish this residue from others despite identical properties. Here, we report strategy that utilizes proximity-driven reactivity achieve selective azo coupling between tyrosine and aryldiazonium. phenylalanine...
Abstract Depleted uranium (DU), when accidentally released from the nuclear industry, can enter human body and cause kidney damage, as DU induces oxidative damage apoptosis through mitochondrial pathways inflammatory reactions. The existing nanoparticles used to treat injury have low bioavailability poor targeting. In this study, mesoporous polydopamine (MPDA), poly‐(ethylene glycol) (PEG), triphenylphosphonium (TPP) are combined develop a novel mitochondrion‐targeting bifunctional...
Site-selective lysine post-translational modifications such as acetylation, methylation, hydroxylation, and isopeptide formation mediate the precise control of important signaling events in cells with unmistakable accuracy. This unparalleled site selectivity (modification a single particular protein proteome) is still challenge for non-enzymatic reactions; difficulty lies differentiation ε-amino group from other reactive groups pinpointing one out many N-terminal amine that have similar...
Alpha-fetoprotein (AFP) is a typical tumor marker in early diagnosis. Highly specific detection of the AFP was demonstrated using AlGaAs/GaAs high electron mobility transistors (HEMTs). Anti-AFP antibody immobilized to Au-coated gate area HEMT by covalent modification method. To avoid any nonspecific binding, blocked bovine serum albumin after reduction carbonyl group. A limit for as low few picograms/milliliter achieved, and cross reactivity sensor found negligible, demonstrating its...
An effective and regioselective strategy for trifluoromethylation perfluoroalkylation of isoquinolines heteroarenes was developed.
Discerning the different interaction states during dynamic protein–ligand binding is difficult. Here we apply site-specific cysteine−α-chloroacetyl cross-linking to scrutinize between Src homology 2 (SH2) domain and phosphotyrosine (pY) peptides, a highly that key cellular signal transduction. From model SH2 protein set of representative domains, showed here proximity-induced reaction cross-linked two spatially adjacent chemical groups as result interaction, reciprocally, information about...
Proteolytic enzymes in the gut represent one of biggest barriers against oral delivery therapeutic proteins and peptides. In current study, we explored effect poly(ethylene glycol) 400 (PEG 400), a commonly used crowding agent, on insulin degradation mediated by α-chymotrypsin (α-CT). Without PEG 400, was quickly cleaved α-CT to generate inactive products. comparison, incorporation resulted reaction mixtures with retained biological activity. The analysis conformation local environment...