A5005655825- Pancreatic function and diabetes
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
Max Planck Institute of Molecular Cell Biology and Genetics
2021-2023
Novo Nordisk Foundation
2018-2023
University of Copenhagen
2018
To decipher the populations of cells present in human fetal pancreas and their lineage relationships, we developed strategies to isolate pancreatic progenitors, endocrine progenitors cells. Transcriptome analysis individual revealed a large degree conservation among vertebrates drivers gene expression changes occurring at different steps differentiation, although notably, sometimes, members same family are expressed. The transcriptome establishes resource identify novel genes pathways...
Basic helix-loop-helix genes, particularly proneural are well-described triggers of cell differentiation, yet information on their dynamics is limited, notably in human development. Here, we focus Neurogenin 3 (NEUROG3), which crucial for pancreatic endocrine lineage initiation. By monitoring both NEUROG3 gene expression and protein single cells using a knockin dual reporter 2D 3D models pancreas development, show an approximately 2-fold slower than that the mouse. We observe heterogeneous...
Summary Basic helix-loop-helix genes, particularly proneural are well-described triggers of cell differentiation, yet limited information exists on their dynamics, notably in human development. Here, we focus Neurogenin 3 ( NEUROG3 ), which is crucial for pancreatic endocrine lineage initiation. Using a double reporter to monitor endogenous transcription and protein expression single cells 2D 3D models pancreas development, show peaks the RNA at 22 11 hours respectively, approximately...