Abstract Gut bacterial microbiome dysbiosis in type 2 Diabetes Mellitus (T2DM) has been reported, but such an association with Diabetic Retinopathy (DR) is not known. We explored possible link between gut and DR. Using fecal samples of healthy controls (HC) people T2DM with/without DR, communities were analysed using 16S rRNA gene sequencing data QIIME R software. Dysbiosis the microbiomes, at phyla genera level, was observed DR compared to HC. People exhibited greater discrimination from...

Studies have documented dysbiosis in the gut mycobiome people with Type 2 diabetes mellitus (T2DM). However, it is not known whether of T2DM patients would be reflected diabetic retinopathy (DR) and if so, observed similar DR. Gut mycobiomes were generated from healthy controls (HC), DR through Illumina sequencing ITS2 region. Data analysed using QIIME R software. Dysbiotic changes compared to HC at phyla genera level. Mycobiomes HC, could discriminated by heat map analysis, Beta diversity...

Purpose: To enumerate the ocular surface fungal microbiome of healthy human eyes by using next-generation sequencing (NGS). Methods: Tarsal and fornix conjunctiva from lower upper lids both individuals were swabbed in duplicate separately. A total 34 samples collected 17 individuals, which used for generation microbiomes NGS. Twenty-four swabs detection culturable fungi conventional cultivable method. Microbiome involved DNA extraction, internal transcribed spacer 2 (ITS2) amplification,...

Keratitis, an inflammatory disease of the eye, when neglected could lead to sight-threatening complications and ultimately blindness. Globally, over a million people are affected by keratitis annually. Keratitis has microbial etiology is caused bacteria, fungi, viruses, etc. The present study compared ocular surface fungal microbiome healthy individuals with keratitis. Fungal microbiomes from conjunctival swabs corneal scrapings were generated using ITS2 region amplicons. Microbiomes...

Purpose: In this study, the gut fungal microbiome of uveitis (UVT) patients was generated and compared with healthy controls (HC) to identify dysbiosis in UVT ascertain role disease pathology. Methods: present microbiomes were analyzed fecal samples HC (n = 24) 14) using high-throughput Illumina sequencing ITS2 region ribosomal RNA. QIIME R software used for data analysis. Results: The richness diversity significantly decreased HC. Our analyses showed enrichment several pathogenic fungi...

Several types of vesicular systems for drug delivery have been developed, each with its own set advantages and applications. Among liposomes niosomes, ufasomes stand out as versatile capable encapsulating a wide range therapeutic agents. Ufasomes, also known unsaturated fatty acid vesicles are composed closed lipid bilayers that formed from acids their ionized species. These unique property maintaining pH 7 to 9, which allows them function effectively in various physiological environments....

Objective: The present study evaluated the antihypercholesterolemic effect of Macrotyloma uniflorum (Lam.) Verdc extract by examining its effects on food consumption, weight gain, serum lipid profile, glutamate oxaloacetate transamianse (SGOT), pyruvate transaminase (SGPT), and body fat in rats. Methods: Animals were fed with experimental high-fat diets (HFD) that contain 10% lard plus, 5% corn oil. Food intake measured. Serum samples analyzed for estimation profiles other biochemical...

Fungi have been associated with various diseases of the eye like keratitis, uveitis and endophthalmitis. Despite this fact, fungal microbiome (mycobiome) studies compared to bacterial remained neglected. In present study, using metagenomic sequencing, mycobiomes vitreous healthy control individuals (VC, n = 15) post fever retinitis + non-PFR (PFR+, 9) were analysed compared. The results indicated that Ascomycota was most predominant phylum in both VC PFR+ groups. Further, at genera level it...

Purpose: To use electronic medical record data to study the altitude, UV exposure, and biogeographical distribution of senile cataract in India.Methods: This is a hospital-based, cross-sectional patients over 40 years old with an ophthalmologist-confirmed diagnosis (cortical, nuclear, posterior subcapsular, or combination) either both eyes. Electronic entered between August 2010 December 2019 were extracted from large multi-tiered ophthalmology network India. Residential districts classified...

Ocular microbiome research has gained momentum in the recent past and provided new insights into health disease conditions. However, studies on sight threatening intraocular inflammatory diseases have remained untouched. In present study, we attempted to identify bacterial associated with post fever retinitis using a metagenomic sequencing approach. For this purpose, ocular microbiomes were generated from vitreous samples collected control individuals (VC, n = 19) (PFR, 9), analysed. The...

Purpose: To analyze the gut bacterial microbiome of streptozotocin-induced diabetic rats and with retinal changes. Methods: Induction diabetes was confirmed by an increase in blood sugar (>150 mg/dL), progression changes assessed histology immunohistochemistry sections. Microbiomes were generated using fecal DNA, V3–V4 amplicons sequenced analyzed QIIME R. Results: Dysbiosis observed at phylum genus levels compared control rats. Heat-map analysis based on differentially abundant genera...

This study aimed to describe the clinical profile and magnitude of diabetic retinopathy (DR) in patients presenting a multitier eye hospital network India.This cross-sectional hospital-based included 263,419 individuals with diabetes mellitus (DM) between February 2012 2021 (9-year period). The data were collected using an electronic medical record (EMR). Patients diagnosis DR at least one analysis. Severe nonproliferative DR/proliferative DR/diabetic macular edema (DME) considered...

Escherichia coli is a predominant bacterium in the intestinal tracts of animals. Phylogenetically, strains have been classified into seven phylogroups, A, B1, B2, C, D, E, and F. Pathogenic categorized several pathotypes such as Enteropathogenic (EPEC), Enterotoxigenic (ETEC), Enteroinvasive (EIEC), Enteroaggregative (EAEC), Diffusely adherent (DAEC), Uropathogenic (UPEC), Shiga-toxin producing (STEC) or Enterohemorrhagic (EHEC) Extra-intestinal pathogenic E. (ExPEC). also survives commensal...

The virome of ocular fluids is naive. results this study highlight the in vitreous fluid eye individuals without any infection and compare it with retinitis. A total 1,016,037 viral reads were generated from 25 samples comprising control post-fever retinitis (PFR) samples. top 10 families comprised Myoviridae, Siphoviridae, Phycodnaviridae, Herpesviridae, Poxviridae, Iridoviridae, Podoviridae, Retroviridae, Baculoviridae , Flaviviridae . Principal coordinate analysis heat map clearly...

Background One of the major challenges in health care sector is that approximately 80% generated data remains unstructured and unused. Since it difficult to handle from electronic medical record systems, tends be neglected for analyses most hospitals centers. Therefore, there a need analyze big systems so we can optimally utilize unearth all unexploited information it. Objective In this study, aimed extract list diseases associated keywords along with corresponding time durations an...

This paper proposes a novel technique of sparsing speech data and compressing it in spectral domain. Discrete Rajan Transform is applied to voice the spectrum sparsed by retaining first component CPI (Cumulative Point Index) forcing other components zero. Thus could be compressed maximum 12.5% original data. As when required synthesized using Inverse reconstructed analyzed for speaker recognition. Speaker recognition accuracy 93.5% has been obtained this case.

A 2D image is a rectangular array of what are called pixels and the pixel values represent intensity or color information corresponding digital at various positions addressed by row column numbers. could also be informally as slice matrix values. 3D generally viewed an ordered sequence slices arranged in z-direction arrangement voxel matroid Most processing operations similar to those processing. images processed with help scanning windows, whereas windows. For instance, operation like...

Inflammation of the cornea is known as keratitis, and bacteria, fungi, protozoans, viruses are etiological agents this disease. Delayed treatment keratitis could result in loss vision and, under certain severity conditions, removal an eye its associated structures. In current study, ocular surface (conjunctiva cornea) mycobiomes individuals with bacterial were compared mycobiome (conjunctiva) healthy individuals, free any morbidity. Mycobiomes generated through NGS approach using...

In the evolving landscape of education, paradigm shift towards online learning has become even more pronounced in response to prevailing global conditions. This transition, while offering unprecedented accessibility and flexibility, brings forth unique challenges, particularly realm assessing monitoring student performance. The absence traditional face-to-face interactions direct observations necessitates innovative solutions gauge predict success effectively. Recognizing imperative need for...