A5087410069- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Genetic Neurodegenerative Diseases
- Biochemical and Structural Characterization
- Prion Diseases and Protein Misfolding
- Autoimmune Neurological Disorders and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Carbohydrate Chemistry and Synthesis
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Peroxisome Proliferator-Activated Receptors
- Neurological Disease Mechanisms and Treatments
- Neurological Disorders and Treatments
- Trace Elements in Health
The University of Texas Southwestern Medical Center
2023-2025
Southwestern Medical Center
2023
Abstract Background Neurodegenerative tauopathies may progress based on seeding by pathological tau assemblies, whereby an aggregate is released from one cell, gains entry to adjacent or connected and serves as a specific template for its own replication in the cytoplasm. Seeding into complex cytoplasmic milieu happens within hours, implying existence of unknown factors that regulate this process. Methods We used proximity labeling identify proteins control seed amplification 5 h exposure....
Distinct tau amyloid assemblies underlie diverse tauopathies but defy rapid classification. Cell and animal experiments indicate functions as a prion, different strains propagated in cells cause unique, transmissible neuropathology after inoculation. Strain amplification requires compatibility of the monomer template. We used cryo–electron microscopy to study one cell-based yellow fluorescent protein (YFP)–tagged strain, resolving its nature. then sequential alanine (Ala) substitution (scan)...
Distinct tau amyloid assemblies underlie diverse tauopathies but defy rapid classification. Cell and animal experiments indicate functions as a prion, different strains propagated in cells cause unique, transmissible neuropathology after inoculation. Strain amplification requires compatibility of the monomer template. We used cryo-EM to study one cell-based YFP-tagged strain, resolving its nature. then sequential alanine (Ala) substitution (scan) within repeat domain (RD) measure...
Neurodegenerative tauopathies may progress based on seeding by pathological tau assemblies, whereby an aggregate is released from one cell, gains entry to adjacent or connected and serves as a specific template for its own replication in the cytoplasm.
<title>Abstract</title> <bold>Background </bold>Neurodegenerative tauopathies may progress based on seeding by pathological tau assemblies, whereby an aggregate is released from one cell, gains entry to adjacent or connected and serves as a specific template for its own replication in the cytoplasm. In vitro reactions typically take days, yet into complex cytoplasmic milieu happens within hours, implicating machinery with unknown players that controls this process acute phase. <bold>Methods...
Abstract Background The prion model of tau propagation in Alzheimer’s Disease predicts that seeds are released from cells and taken up by neighboring cells, resulting spreading the disease. Our previous work revealed aggregates bind to heparan sulfate proteoglycans (HSPGs) on cell surface, followed cellular uptake via macropinocytosis. HSPGs glycoproteins, consisting a protein core decorated with linear glycosaminoglycan (GAG) chains called (HS) highly variable sulfation patterns. We have...
Abstract Pediatric and adult autoimmune encephalitis (AE) are often associated with Abs to the NR1 subunit of N-methyl-d-aspartate (NMDA) receptor (NMDAR). Very little is known regarding cerebrospinal fluid humoral immune profile Ab genetics pediatric anti–NMDAR-AE. Using a combination cellular, molecular, immunogenetics tools, we collected from subjects generated 1) flow cytometry data calculate frequency B cell subtypes in anti–NMDAR-AE controls, 2) panel recombinant human case that was...