A5003998406- Glioma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Proteins in Food Systems
- Shoulder Injury and Treatment
- Microencapsulation and Drying Processes
- Adenosine and Purinergic Signaling
- Meningioma and schwannoma management
- Protein purification and stability
- Muscle activation and electromyography studies
- Chromatin Remodeling and Cancer
- Radiomics and Machine Learning in Medical Imaging
- Childhood Cancer Survivors' Quality of Life
- Alzheimer's disease research and treatments
- Neonatal Health and Biochemistry
- Coffee research and impacts
- Infant Nutrition and Health
- Mechanics and Biomechanics Studies
- Acute Lymphoblastic Leukemia research
- Orthopaedic implants and arthroplasty
- Parkinson's Disease Mechanisms and Treatments
- Nerve Injury and Rehabilitation
- Vascular Malformations Diagnosis and Treatment
- Enzyme Catalysis and Immobilization
- Botulinum Toxin and Related Neurological Disorders
- Genetic factors in colorectal cancer
Universitätsmedizin Göttingen
2015-2025
Laboratoire de Biomécanique et Mécanique des Chocs
2021-2023
University of Göttingen
2009-2022
German Primate Center
2022
Université de Montréal
2017-2022
Université Claude Bernard Lyon 1
2021
Université Gustave Eiffel
2021
University of Alberta
2018-2019
Maison de la Simulation
2019
University of Colorado Denver
2018
Purpose Diffuse intrinsic pontine glioma (DIPG) is a brainstem malignancy with median survival of < 1 year. The International and European Society for Pediatric Oncology DIPG Registries collaborated to compare clinical, radiologic, histomolecular characteristics between short-term survivors (STSs) long-term (LTSs). Materials Methods Data abstracted from registry databases included patients North America, Australia, Germany, Austria, Switzerland, the Netherlands, Italy, France, United...
The novel entity of "diffuse midline glioma, H3 K27M-mutant" has been defined in the 2016 revision World Health Organization (WHO) classification tumors central nervous system (CNS). Tumors this arise CNS structures predominantly pediatric patients and are associated with an overall dismal prognosis. They by K27M mutations H3F3A or HIST1H3B/C, encoding for histone 3 variants H3.3 H3.1, respectively, which considered hallmark events driving gliomagenesis. Here, we characterized 85 centrally...
The heat-induced aggregation of bovine β-lactoglobulin, dispersed in water at neutral pH and different concentrations (10, 30, or 50 g dry matter/L), was studied 65 °C, the results are related to a kinetic model. Native PAGE SDS−PAGE analysis under nonreducing reducing conditions showed that on heating disulfide-linked aggregates were formed average size these increased with increasing initial β-lactoglobulin concentration. In presence thiol-blocking agent N-ethylmaleimide (NEM), molar ratio...
The effect of pH in the range 6.0−8.0 on denaturation and aggregation β-lactoglobulin (β-lg) was investigated. Results were interpreted terms reaction scheme for β-lg proposed by Roefs De Kruif (Eur. J. Biochem. 1994, 226, 883−889). rate conversion native increased strongly at higher values, whereas molecular mass aggregates decreased strongly. In 6.4−8.0 formed mainly intermolecular disulfide bonds, but even 6.0, thiol/disulfide exchange reactions involved, although to a lesser extent. time...
The aggregates in heated bovine β-lactoglobulin solutions were separated by high-performance size-exclusion chromatography, and molecular characteristics measured on-line using multiangle laser-light scattering detection (SEC-MALLS). This technique was proven to be a very useful valuable approach for characterization of heat-induced aggregates. Using TSK G2000 SWXL G4000 silica gel columns connected series, up mass 4 × 106 Da could separated, complete distributions derived. Up 1.5 the masses...
Summary In situ light scattering, where scattered from a sample is measured directly while the heated in instrument, presented as simple and effective technique for studying heat-induced aggregation of β-lactoglobulin. This was shown to be applicable not only monitoring initial steps, but following overall process with time. The experiments gave results similar measurements carried out after heat-quench treatment, were more informative. From on standard NIZO β-lactoglobulin sample, strongly...
Diffuse intrinsic pontine glioma (DIPG) is a rare and deadly childhood malignancy. After 40 years of mostly single-center, often non-randomized trials with variable patient inclusions, there has been no improvement in survival. It therefore time for international collaboration DIPG research, to provide new hope children, parents medical professionals fighting DIPG. In first step towards collaboration, 2011, network biologists clinicians working the field was established within European...
α-Synuclein is a protein that aggregates as amyloid fibrils in the brains of patients with Parkinson's disease and dementia Lewy bodies. Small oligomers α-synuclein are neurotoxic thought to be closely associated disease. Whereas fibrillization fibril morphologies have been studied extensively various methods, earliest stages aggregation properties oligomeric intermediates less well understood because few methods able detect characterize early-stage aggregates. We used fluorescence...
Rapid acetone fractionation of crude yeast extract at low temperature separates carboxypeptidase Y inhibitor from the Y-inhibitor complex. On a protein basis has been purified 890-fold, resulting in homogeneity as determined by disc electrophoresis and filtration on Sephadex G-75. The molecular weight was calculated to be about 25,000. is heat-labile extracts, whereas form it loses only 11% its activity when heated 100 degrees for 5 min. inactivated proteinases A (EC 3.4.23.8) B 3.4.22.9),...
Abstract Pediatric high-grade glioma (pedHGG) can occur as first manifestation of cancer predisposition syndromes resulting from pathogenic germline variants in the DNA mismatch repair (MMR) genes MSH2 , MSH6 MLH1 and PMS2 . The aim this study was to establish a generalized screening for Lynch syndrome constitutional MMR deficiency (CMMRD) pedHGG patients, detection deficiencies (MMRD) may enable upfront therapeutic use checkpoint inhibitors identification variant carriers patients’...
We give a quantitative treatment of the destabilization three types milk protein dispersions. For this we consider proteins as adhesive-hard-sphere bio-colloids. If attractive interactions become strong enough system passes percolation threshold and gels. Macroscopic properties these gels are studied by measuring viscoelasticity permeability coefficients. These coefficients related to structural (fractal) which were measured using scattering confocal scanning laser microscopy (CLSM)...
Pre-fibrillar oligomers of α-synuclein are thought to be pathogenic molecules leading neurotoxicity associated with Parkinson's disease and other neurodegenerative disorders. However, small difficult isolate for study. To gain better insight into the properties oligomers, we investigated engineered specific size (dimers, tetramers, octamers) linked head-to-tail in tandem, comparing behavior monomeric α-synuclein. All oligomeric constructs remained largely disordered solution, as determined...
Abstract Background The term gliomatosis cerebri (GC), a radiology-defined highly infiltrating diffuse glioma, has been abandoned since molecular GC-associated features could not be established. Methods We conducted multinational retrospective study of 104 children and adolescents with GC providing comprehensive clinical (epi-)genetic characterization. Results Median overall survival (OS) was 15.5 months (interquartile range, 10.9–27.7) 2-year rate 28%. Histopathological grading correlated...
The aim of the present analysis was to assess feasibility, toxicity, and tumor control reirradiation as a salvage treatment for progressive pediatric non-pontine high-grade gliomas (HGG). database Reference Center Radiation Oncology German HIT (HIT = acronym brain tumor) network childhood tumors screened children who were reirradiated HGG. We identified eight patients (WHO grade III: n 5; WHO IV: 3) underwent between April 2006 July 2012. Median age 13.5 years at primary diagnosis 14.8 first...
Recent research identified histone H3 K27M mutations to be associated with a dismal prognosis in pediatric diffuse midline glioma (pDMG); however, data on detailed MRI characteristics respect K27 mutation status and molecular subgroups (H3.1 H3.3 mutations) are limited.Standardized magnetic resonance imaging (MRI) parameters epidemiologic of 68 pDMG patients (age <18 years) were retrospectively reviewed compared a) mutant versus wildtype (WT) tumors b) H3.1 tumors.Intracranial gliomas (n =...
Abstract NAD(P)H oxidase is a major endogenous source of reactive oxygen species (ROS). ROS may not only be involved in carcinogenesis but also efficacy chemotherapeutic agents like doxorubicin. By comprehensive genotyping approach covering 48 genetic polymorphisms (single-nucleotide polymorphisms) five subunits phagocytic oxidase, we asked whether they affect gene expression, enzymatic activity, and outcome CHO(E)P chemotherapy. A highly consistent effect was observed for the CYBA...
Tryptophan synthase was purified from Saccharomyces cerevisiae with the aid of affinity chromatography up to a specific activity 525 units/g protein. Electrophoresis on polyacrylamide gel showed one main protein component. Gel filtration Sephadex G‐150 indicates an average molecluar weight 143000. Dissociation dodecylsulfate followed by dodecyl‐sulfate‐acrylamide electrophoresis leads conclusion that enzyme is probably composed four subunits equal size. The K m ‐values respect its substrates...
The denaturation and aggregation of β-lactoglobulin was studied by isothermal calorimetry. Experiments with several concentrations (15−100 g/L) were performed at temperatures in the range 62−68.5 °C. Even a small change temperature had tremendous effect on shape thermograms, depending also very strongly concentration used. measured thermograms modeled using kinetic model for recently developed Roefs De Kruif. In this we recognize four consecutive steps: dissociation, unfolding, exchange...
Accurate muscle geometry (muscle length and moment arm) is required to estimate function when using musculoskeletal modelling. In shoulder, muscles are often modelled as a collection of independent line segments, leading non-physiological trajectory, especially for the rotator cuff muscles. To prevent this, surface mesh model was developed validated against 7 MRI positions in one participant. Mean arm errors 11.4% vs. 8.8% model. While with lines led some trajectories, gave lower...
Abstract Background Our aim is to investigate the association of treatment with survival in patients diffuse intrinsic pontine glioma (DIPG) by examining 6 historical paths. Methods We retrospectively analyzed data from 409 radiologically centrally reviewed DIPG, sourced German Society Pediatric Oncology and Hematology HIT-HGG trial database SIOPE-DIPG/DMG Registry. Survival outcomes were estimated using Kaplan–Meier method, univariable multivariable Cox proportional hazard models study...
Objectives The transforming growth factor-β (TGFB) pathway has substantial impact on cellular functions, cell proliferation, and apoptosis. We used bioinformatics, gene expression, biological assays to evaluate the functionality of frequent inherited germline polymorphisms in TGFB receptor 1 (TGFBR1). Methods In an exploratory (n=55) confirmatory (n=106) study, we analyzed TGFB1 after incubation with TGFβ1 ligand exposure X-rays peripheral blood human mononuclear cells. Expression genes was...
Accurate muscle geometry is essential to estimate moment arms in musculoskeletal models. Given the complex interactions between shoulder structures, we hypothesized that finite element (FE) modelling suitable obtain physiological trajectory. A FE glenohumeral joint model was developed based on medical imaging. Moment were computed and compared literature MRI-based estimation. Our produces consistent with MRI data (max 17 mm differences). The inferior superior fibres of a same can have...