A5073445415- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Complement system in diseases
- Antibiotic Resistance in Bacteria
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Aquaculture disease management and microbiota
- Virology and Viral Diseases
- Invertebrate Immune Response Mechanisms
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- COVID-19 Clinical Research Studies
- Bacterial biofilms and quorum sensing
- Infections and bacterial resistance
- Viral Infectious Diseases and Gene Expression in Insects
- interferon and immune responses
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- Vaccine Coverage and Hesitancy
Instituto Nacional de Salud Pública
2008-2024
Background: The study of human B cell response to dengue virus (DENV) infection is critical understand serotype-specific protection and the cross-reactive sub-neutralizing response.Whereas first beneficial thus represents ultimate goal vaccination, latter has been implicated in development severe disease, which occurs a small, albeit significant, fraction secondary DENV infections.Both primary infections are associated with production poly-reactive IgG antibodies.Methods: To gain insight...
Background: Despite the potential to produce antibodies that can neutralize different virus (heterotypic neutralization), there is no knowledge of why vaccination against influenza induces protection predominantly utilized viral strains (homotypic response).Identification structural patterns B cell repertoire associated heterotypic neutralization may contribute identify relevant epitopes for a universal vaccine influenza.Methods: Blood samples were collected from volunteers immunized with...
Hypermucoviscosity (hmv) is a capsule-associated phenotype usually linked with hypervirulent Klebsiella pneumoniae strains. The key components of this are the RmpADC proteins contained in non-transmissible plasmids identified and studied K. . variicola closely related to recently has been as an emergent human pathogen. variicol normally contains plasmids, some them carrying antibiotic resistance virulence genes. Previously, we described clinical isolate showing hmv-like that harbors 343-kb...
Influenza viruses display a high mutation rate and complex evolutionary patterns. Next-generation sequencing (NGS) has been widely used for qualitative semi-quantitative assessment of genetic diversity in biological samples. The "deep sequencing" approach, enabled by the enormous throughput current NGS platforms, allows identification rare viral variants targeted regions, but is usually limited to small number samples.We designed proof-of-principle study test whether redistributing from...
The E7 protein of high risk HPV types has been found with different molecular weights, mainly because phosphorylation, an event that changes charge and mobility in SDS-PAGE. Distribution the cellular compartments also subject debate as some groups report nucleus others cytoplasm. subcellular distribution weights reported for suggest presence isoforms. We examined this possibility by using several antibodies recognize epitopes on HPV-16 protein. showed is processed 3 isoforms isoelectric...
Carbapenem-resistant Gram-negative bacteria isolated in Venezuela have been poorly characterized. The present study characterized a total of 34 isolates obtained from 27 patients; five these patients were multi-infected. bacterial species identified Klebsiella pneumoniae (17), Pseudomonas aeruginosa (9), and Acinetobacter baumannii (8). From isolates, 85% as carbapenemase-producing bacteria, the carbapenemase genes blaKPC-2 (10/29 [34.4%]), blaVIM-type (7/29 [24.1%]), blaOXA-23 blaNDM-1...
For malaria transmission, Plasmodium parasites must develop in the mosquito vector. Oxidative stress insect midgut, triggered by environmental changes (e.g., pH and temperature), influences cellular signaling involved differentiation from gametocytes to mobile ookinetes for purpose of parasite survival. activates homeostatic response characterized phosphorylation eIF2α, attenuation protein synthesis, transcription genes participating unfolded antioxidant processes, forming a part an...
High-throughput sequencing of the antibody repertoire is enabling a thorough analysis B cell diversity and clonal selection, which may improve novel discovery process. Theoretically, an adequate bioinformatic could allow identification candidate antigen-specific antibodies, requiring their recombinant production for experimental validation specificity. Gene synthesis commonly used generation antibodies identified in silico. Novel strategies that bypass gene offer more accessible...
Background The axolotl, Ambystoma mexicanum is a unique biological model for complete tissue regeneration. Is neotenic endangered species and highly susceptible to environmental stress, including infectious disease. In contrast other amphibians, the axolotl particularly vulnerable certain viral infections. Like salamanders, genome one of largest (32 Gb) impact size on Ig loci architecture unknown. To better understand immune response in we aimed characterize immunoglobulin A. compare it with...
Antibody class switch recombination (CSR) to IgG, IgA or IgE is a hallmark of adaptive immunity, allowing antibody function diversification beyond IgM. CSR involves deletion the IgM/IgD constant region genes placing new acceptor Constant gene, downstream VDJH exon. depends on non-coding (CSRnc) transcription donor I and IH exons, located 5' upstream each CH coding gene. Although our knowledge role CSRnc has advanced greatly, its extension importance in healthy diseased humans scarce. We...
The immune response to SARS-CoV-2 has been extensively studied following the pandemic outbreak in 2020; however, presence of specific T cells against before vaccination not evaluated Mexico. In this study, we estimated frequency CD4+ and CD8+ that exhibit a S (spike) N (nucleocapsid) proteins Mexican population. We collected 78 peripheral blood samples from unvaccinated subjects, antibodies spike (RBD) protein was determined. Peripheral mononuclear were isolated stimulated with pool or...
Abstract Background Antibody class switch recombination (CSR) to IgG, IgA or IgE is a hallmark of adaptive immunity, allowing antibody function diversification beyond IgM. CSR involves deletion the IgM/IgD constant region genes placing new acceptor Constant (C H ) gene, downstream VDJ exon. depends on non-coding (CSRnc) transcription donor Iμ and I exons, located 5’ upstream each C coding gene. Although our knowledge role CSRnc has advanced greatly, its extension importance in healthy...
Abstract Background The axolotl, Ambystoma mexicanum is a unique biological model for complete tissue regeneration. Is neotenic endangered species and highly susceptible to environmental stress, including infectious disease. In contrast other amphibians, the axolotl particularly vulnerable certain viral infections. Like salamanders, genome one of largest (32 Gb) impact size on Ig loci architecture unknown. To better understand immune response in we aimed characterize immunoglobulin A....