A5023278950- Cannabis and Cannabinoid Research
- Metabolomics and Mass Spectrometry Studies
- GABA and Rice Research
- Pancreatic function and diabetes
- Neurotransmitter Receptor Influence on Behavior
- Analytical Chemistry and Chromatography
- Eicosanoids and Hypertension Pharmacology
- Alcohol Consumption and Health Effects
- Fatty Acid Research and Health
- Edible Oils Quality and Analysis
- Coffee research and impacts
- Adipose Tissue and Metabolism
- Plant Parasitism and Resistance
- Mass Spectrometry Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Spectroscopy and Chemometric Analyses
- Plant tissue culture and regeneration
- Fermentation and Sensory Analysis
- Vitamin C and Antioxidants Research
- Phytochemicals and Antioxidant Activities
- Peroxisome Proliferator-Activated Receptors
- Skin Protection and Aging
- Adenosine and Purinergic Signaling
- Biochemical Analysis and Sensing Techniques
- Vitamin D Research Studies
Hospital Universitario Reina Sofía
2011-2014
University of Córdoba
2010-2014
Instituto Maimónides de Investigación Biomédica de Córdoba
2011-2014
The mechanism of action cannabidiol (CBD), the main non-psychotropic component Cannabis sativa L., is not completely understood. First assumed that compound was acting via cannabinoid CB2 receptors (CB2Rs) it now suggested interacts with non-cannabinoid G-protein-coupled (GPCRs); however, CBD does bind high affinity to orthosteric site any GPCR. To search for alternative explanations, we tested as a potential allosteric ligand CB2R. Radioligand and non-radioactive homogeneous binding,...
Cannabigerol (CBG) is one of the major phytocannabinoids present in Cannabis sativa L. that attracting pharmacological interest because it non-psychotropic and abundant some industrial hemp varieties. The aim this work was to investigate parallel binding properties CBG cannabinoid CB1 (CB1R) CB2 (CB2R) receptors effects compound on agonist activation those CB1-CB2 heteroreceptor complexes. Using [3H]-CP-55940, competed with low micromolar Ki values CB1R CB2R. Homogeneous living cells, which...
Background and Purpose Phytocannabinoids are produced in Cannabis sativa L. acidic form decarboxylated upon heating, processing storage. While the biological effects of cannabinoids such as Δ 9 ‐tetrahydrocannabinol have been extensively investigated, bioactivity ‐tetahydrocannabinol acid (Δ ‐THCA) is largely unknown, despite its occurrence different preparations. Here we assessed possible neuroprotective actions ‐THCA through modulation PPARγ pathways. Experimental Approach The six...
Most research on the extraction of high-priced compounds from vineyard/wine byproducts has traditionally been focused grape seeds and skins as raw materials. Vine-shoots can represent an additional source to those materials, characteristics which could depend cultivar. A comparative study hydroalcoholic extracts 18 different vineyard cultivars obtained by superheated liquid (SHLE), microwave-assisted (MAE), ultrasound-assisted (USAE) is here presented. The optimal working conditions for each...
Abstract Neuroprotective M2‐skewed microglia appear as promising to alter the course of neurodegenerative diseases and G protein‐coupled receptors (GPCRs) are potential targets achieve such microglial polarization. A common feature adenosine 2A (A R) cannabinoid CB 2 (CB GPCRs in is that their expression upregulated Alzheimer's disease (AD). On one hand, R seems a target for neuroprotection, delaying processes like those associated AD or Parkinson's diseases. antagonists reduce amyloid...
We have here assessed, using Δ9-tetrahydrocannabinol (Δ9-THC) for comparison, the effect of Δ9-tetrahydrocannabinolic acid (Δ9-THCA) and Δ9-tetrahydrocannabivarin (Δ9-THCV) that is mediated by human versions CB1, CB2, CB1-CB2 receptor functional units, expressed in a heterologous system. Binding to CB1 CB2 receptors was addressed living cells means homogeneous assay. A biphasic competition curve binding receptor, obtained Δ9-THCV expressing two receptors. Signaling studies included cAMP...
Medical cannabis (Cannabis sativa L.) is a source of bioactive phytochemicals with promising pharmacological and therapeutic applications. Enhancing the accumulation valuable compounds potentially way increasing economic importance this crop. Signaling molecules like salicylic acid (SA), jasmonic (JA), γ-aminobutyric (GABA) are involved in regulation plant development responses to biotic abiotic stresses. Moreover, several phytohormones regulate trichome formation elicit synthesis secondary...
Intravenous pharmacokinetics and oral bioavailability of cannabidiol (CBD) with different formulations have not been investigated in horses may represent a starting point for clinical studies.To describe after intravenous administrations oil micellar simulate treatments.Single experiment two-way randomised experiments, Latin-square design.Eight healthy received CBD at 1.00 mg/kg dose, sesame formulation, both 10.00 mg/kg. Concentrations were measured using LC-MS/MS fitted by nonlinear mixed...
The substantial increase in legalization and subsequent regulation of cannabis has intensified the control analytical monitoring products to assure sample quality cannabinoid content crop. In this sense, restriction on cultivating legal plants been limited 0.2-0.3% Δ9-THC content, depending host country's laws. Thereby, flowers containing more than limit are considered illicit drug-type cultivations require obtention specific permits work with them. official method established by European...
The aim of this study was to propagate axillary shoots Cannabis sativa L. using liquid medium in temporary immersion bioreactors. effect frequency (3 or 6 immersions per day), explant type (apical basal sections), number (8, 10, and 16 explants), mineral (Murashige Skoog half-strength nitrates, β -A -H, all supplemented with 2-μM metatopoline), sucrose supplementation (2, 0.5, 0% sucrose), culture duration (4 weeks), bioreactor (RITA® Plantform™) were investigated. As a result, we propose...
(−)-Δ9-trans-tetrahydrocannabinol (THC), which is the principal psychoactive constituent of Cannabis, mediates its action by binding to two members G-protein-coupled receptor (GPCR) family: cannabinoid CB1 (CB1R) and CB2 (CB2R) receptors. Molecular dynamics simulations showed that pentyl chain THC could adopts an I-shape conformation, filling intracellular cavity between Phe3.36 Trp6.48 for initial agonist-induced activation, in CB1R but not CB2R. This opens five-carbon conformational change...