A5045872030- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Poxvirus research and outbreaks
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
University of Tübingen
2020-2024
University Children's Hospital Tübingen
2024
The Orf virus (ORFV) is a promising vector platform for the generation of vaccines against infectious diseases and cancer, highlighted by its progression to clinical testing phases. One critical steps during GMP manufacturing clarification crude harvest because enveloped nature large size ORFV. This study presents first description ORFV process from HEK suspension batch process. We examined various filter materials, membrane pore sizes, timings, nuclease treatments. Employing Ambr® crossflow...
Prime-2-CoV_Beta is a novel Orf virus (ORFV)-based COVID-19 vaccine candidate expressing both the nucleocapsid and spike proteins of SARS-CoV-2 with receptor-binding domain (RBD) Beta strain. This was shown to be safe immunogenic in first-in-human Phase I clinical trial. With shift immune landscape toward Omicron variant widespread vaccine- and/or infection-derived immunity, further pre-clinical research needed characterize Prime-2-CoV. Here, we quantified humoral cellular response...
Abstract Among the common strategies to design next-generation COVID-19 vaccines is broadening antigenic repertoire thereby aiming increase efficacy against emerging variants of concern (VoC). This study describes a new Orf virus-based vector (ORFV) platform multiantigenic vaccine targeting SARS-CoV-2 spike and nucleocapsid antigens. Vaccine candidates were engineered, either expressing protein (ORFV-S) alone or co-expressing (ORFV-S/N). Mono- elicited comparable levels spike-specific...
Abstract The next generation of COVID-19 vaccines needs to broaden the antigenic repertoire improve breadth immune response and efficacy against emerging variants concern. This study describes a new parapoxvirus-based vector (ORFV) as platform design multi-antigenic vaccine targeting SARS-CoV-2 spike nucleocapsid antigens. Two candidates were engineered, one expressing protein alone (ORFV-S) other co-expressing more conserved (ORFV-S/N). Both elicited comparable levels spike-specific...
Steric exclusion chromatography (SXC) is a promising purification method for biological macromolecules such as the Orf virus (ORFV) vector. The method's principle closely related to conventional polyethylene glycol (PEG) precipitation, repeatedly implementing membranes porous chromatographic media. In past decade, several tasks with SXC showed exceptionally high yields and impurity removal. However, effect of varying process parameters, on precipitation success its limitations SXC, not yet...
The Orf virus (ORFV) is a promising candidate for vector vaccines as well immunomodulatory and oncolytic therapies. However, few publications are available on its infectivity degradation or suitable additives prolonging viral stability. In this study, the non-supplemented ORFV itself showed very high stability at storage temperatures up to 28 °C, with linear titer loss of 0.10 log infectious particles per day 4 °C over period five weeks. To prolong inherent stability, thirty additives, i.e.,...