A5076486128- Fungal and yeast genetics research
- Monoclonal and Polyclonal Antibodies Research
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Magnesium in Health and Disease
- Autophagy in Disease and Therapy
- Plant-Microbe Interactions and Immunity
- Mitochondrial Function and Pathology
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Plant and Fungal Interactions Research
- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Plant Pathogens and Resistance
- Evolutionary Algorithms and Applications
- Mycotoxins in Agriculture and Food
- Biofuel production and bioconversion
- Aluminum toxicity and tolerance in plants and animals
- Advanced Materials and Mechanics
Jawaharlal Nehru Centre for Advanced Scientific Research
2018-2025
Maharaja Sayajirao University of Baroda
2016-2022
Genomic rearrangements associated with speciation often result in variation chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres M. sympodialis as 3–5-kb long kinetochore-bound regions that span an AT-rich core are depleted of the canonical histone H3. Centromeres similar sequence features were CENP-A-rich furfur, which has seven chromosomes, H3 slooffiae...
Candida albicans is the most prevalent fungal pathogen associated with candidemia. Similar to other fungi, complex life cycle of C. has been challenging study high-resolution microscopy due its small size. Here, we employed ultrastructure expansion (U-ExM) directly visualise subcellular structures at high resolution in yeast and during transition hyphal growth. N-hydroxysuccinimide (NHS)-ester pan-labelling combination immunofluorescence via snapshots various mitotic stages provided a...
Emerging studies hint at the roles of autophagy-related proteins in various cellular processes. To understand if influence genome stability, we sought to examine a cohort 35 autophagy mutants Saccharomyces cerevisiae. We observe cells lacking Atg11 show poor mitotic stability minichromosomes. Single-molecule tracking assays and live cell microscopy reveal that molecules dynamically localize spindle pole bodies (SPBs) microtubule (MT)-dependent manner. Loss leads delayed cycle progression....
Magnaporthe oryzae is an important fungal pathogen that causes a loss of 10% to 30% the annual rice crop due devastating blast disease. In most organisms, kinetochores are clustered together or arranged at metaphase plate facilitate synchronized anaphase separation sister chromatids in mitosis. this study, we showed initially separate and position randomly prior M. . Centromeres occupy large genomic regions form on AT-rich DNA without any common sequence motifs. Overall, study identified...
Abstract Candida albicans is the most prevalent fungal pathogen isolated from patients with candidemia. As case for many other fungi, complex life cycle of C. has been challenging to study high-resolution microscopy techniques due its small size. We employed ultrastructure expansion (U-ExM) directly visualise sub-cellular structures at high resolution in yeast and during transition hyphal growth. NHS-ester pan-labelling combination immunofluorescence (IF) provided first comprehensive map...
Magnaporthe oryzae, the causative organism of rice blast, infects cereal crops and grasses at various stages plant development. A comprehensive understanding its metabolism implications on pathogenesis is necessary for countering this devastating crop disease. We present role CorA magnesium transporters, MoAlr2 MoMnr2, in development pathogenicity M. oryzae. The MoALR2 MoMNR2 genes individually complement Mg2+ uptake defects a S. cerevisiae transporter double mutant. respond to extracellular...
Abstract The eukaryotic cell division machinery must rapidly and reproducibly duplicate partition the cell’s chromosomes in a carefully coordinated process. However, chromosome numbers vary dramatically between genomes, even on short evolutionary timescales. We sought to understand how mitotic senses responds karyotypic changes by using series of budding yeast strains which native have been successively fused. Using combination biological profiling, genetic engineering experimental...
Glutathione, an important redox buffer of the cell, also functions as a source sulphur and nitrogen under starvation conditions. The metabolism maintenance glutathione homeostasis are vital for appropriate functioning cell. In addition to γ-glutamyl transpeptidase, fungus-specific alternative pathway involving DUG1, DUG2 DUG3 genes mediate degradation. Here, we studied functional significance in vegetative growth infection cycle cereal blast fungus Magnaporthe oryzae . Cells lacking gene...
Abstract Intra-chromosomal or inter-chromosomal genomic rearrangements often lead to speciation (1). Loss gain of a centromere leads alterations in chromosome number closely related species. Thus, centromeres can enable tracing the path evolution from ancestral derived state (2). The Malassezia species complex phylum Basiodiomycota shows remarkable diversity ranging between six and nine chromosomes (3–5). To understand these transitions, we experimentally identified all eight as binding...
Aging is associated with altered mitochondrial function, which dependent on the magnesium (Mg+2 ) ion flux. The molecular mechanism underlying Mg+2 homeostasis, especially during aging has not been well understood. We previously demonstrated that absence of a vacuolar transporter Mnr2 accelerates cell death in older part colony Magnaporthe oryzae presumably due to an homeostasis. Here, we show localization as dynamic puncta at membrane, cells. Such are often localized close proximity...
Abstract Emerging studies hint at the roles of autophagy-related proteins in various cellular processes a eukaryotic cell. To understand if influence genome stability, we examined cohort 35 autophagy mutants Saccharomyces cerevisiae. We observed cells lacking Atg11 show poor mitotic stability minichromosomes. molecules dynamically localize to spindle pole bodies (SPBs). Loss leads delayed cell cycle progression. Such accumulate metaphase an elevated temperature that is relieved when assembly...
The eukaryotic cell division machinery must rapidly and reproducibly duplicate partition the cell's chromosomes in a carefully coordinated process. However, chromosome number varies dramatically between genomes, even on short evolutionary timescales. We sought to understand how mitotic senses responds karyotypic changes by using series of budding yeast strains which native have been successively fused. Using combination biological profiling, genetic engineering, experimental evolution, we...
Abstract A series of well-synchronized events mediated by kinetochore-microtubule interactions ensure faithful chromosome segregation in eukaryotes. Centromeres scaffold kinetochore assembly and are among the fastest evolving chromosomal loci terms DNA sequence, length, organization intrinsic elements. Neither centromere structure nor dynamics is well studied plant pathogenic fungi. Here, we sought to understand process rice blast fungus, Magnaporthe oryzae . High-resolution confocal imaging...
Abstract Aging is associated with altered mitochondrial function. Mitochondrial function dependent on the magnesium (Mg +2 ) ion flux. The molecular mechanism underlying Mg homeostasis, especially during aging has not been well understood. We previously demonstrated that absence of a vacuolar transporter Mnr2 accelerates cell death in older part colony Magnaporthe oryzae presumably due to an homeostasis. Localization as dynamic puncta at membrane cells further suggests its association aged...