A5038790332- Phagocytosis and Immune Regulation
- Invertebrate Immune Response Mechanisms
- Cell death mechanisms and regulation
- Erythrocyte Function and Pathophysiology
- Autophagy in Disease and Therapy
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Immune responses and vaccinations
- Mosquito-borne diseases and control
- FOXO transcription factor regulation
- Blood Coagulation and Thrombosis Mechanisms
- Ion Channels and Receptors
- Immune cells in cancer
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- CRISPR and Genetic Engineering
- Complement system in diseases
- Pancreatitis Pathology and Treatment
- Diverse Scientific Research Studies
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
Scripps Research Institute
2009-2020
Medical Research Council
2007-2014
MRC Laboratory for Molecular Cell Biology
2002-2014
University College London
2002-2008
Harvard University
1996-2001
Massachusetts General Hospital
1996-2001
Modèles Insectes de l'Immunité Innée
1996
Institut de Biologie Moléculaire et Cellulaire
1996
Macrophages in the Drosophila embryo are responsible for phagocytosis of apoptotic cells and competent to engulf bacteria. Croquemort (CRQ) is a CD36-related receptor expressed exclusively on these macrophages. Genetic evidence showed that crq was essential efficient corpses but not required engulfment The expression CRQ regulated by amount apoptosis. These data define distinct pathways bacteria .
Phagocytosis is an ancient mechanism central to both tissue homeostasis and immune defense. Both the identity of receptors that mediate bacterial phagocytosis nature interactions between other defense mechanisms remain elusive. Here, we report Croquemort (Crq), a Drosophila member CD36 family scavenger receptors, required for microbial efficient clearance. Flies mutant crq are susceptible environmental microbes during development succumb variety infections as adults. Crq acts parallel Toll...
Apoptosis, a genetically programmed cell death, allows for homeostasis and tissue remodelling during development of all multi-cellular organisms. Phagocytes swiftly recognize, engulf digest apoptotic cells. Yet, to date the molecular mechanisms underlying this phagocytic process are still poorly understood. To delineate clearance in Drosophila, we have carried out deficiency screen identified three overlapping phagocytosis-defective mutants, which delete fly homologue ced-12 gene, known as...
Billions of cells die in our bodies on a daily basis and are engulfed by phagocytes. Engulfment, or phagocytosis, can be broken down into five basic steps: attraction the phagocyte, recognition dying cell, internalization, phagosome maturation, acidification. In this study, we focus last two steps, which collectively considered corpse processing, material is degraded. We use Drosophila ovarian follicle as model for engulfment apoptotic epithelial cells. show that processed using canonical...
Clostridium difficile infections (CDIs) cause severe and occasionally life-threatening diarrhea. Hyper-virulent strains produce CDT, a toxin that ADP-ribosylates actin monomers inhibits polymerization. We created transgenic Drosophila lines expressing the catalytic subunit CDTa to investigate its interaction with host signaling pathways in vivo. When expressed midgut, reduces body weight fecal output compromises survival, suggesting impairment of digestive functions. At cellular level,...
Apoptosis, a genetically programmed cell death, allows for homeostasis and tissue remodelling during development of all multi-cellular organisms.Phagocytes swiftly recognize, engulf digest apoptotic cells.Yet, to date the molecular mechanisms underlying this phagocytic process are still poorly understood.To delineate clearance in Drosophila, we have carried out deficiency screen identified three overlapping phagocytosis-defective mutants, which delete fly homologue ced-12 gene, known as...
Abstract Calcium plays a key role as second messenger in many cell types, but for calcium signaling during apoptotic removal is unclear. Using studies lines and the context of whole organism, we demonstrate that recognition induces both an acute sustained flux within phagocytes such essential engulfment. Furthermore, provide evidence release from endoplasmic reticulum entry extracellular via CRAC channels into are important Moreover, knockdown C. elegans stim-1 jph-1, two genes linked to...