A5004144130- Lymphatic System and Diseases
- T-cell and B-cell Immunology
- Bacterial biofilms and quorum sensing
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Childhood Cancer Survivors' Quality of Life
- Food Safety and Hygiene
- Medical research and treatments
- Myasthenia Gravis and Thymoma
- Hematopoietic Stem Cell Transplantation
- Cancer, Lipids, and Metabolism
- Plant biochemistry and biosynthesis
- Eosinophilic Disorders and Syndromes
- Musculoskeletal synovial abnormalities and treatments
- Clusterin in disease pathology
- Plant tissue culture and regeneration
- Microfluidic and Bio-sensing Technologies
- Seaweed-derived Bioactive Compounds
- Immune Cell Function and Interaction
- Bacillus and Francisella bacterial research
- Antibiotic Resistance in Bacteria
- Circadian rhythm and melatonin
- Plant Gene Expression Analysis
- Antimicrobial Resistance in Staphylococcus
- Cancer, Hypoxia, and Metabolism
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2020-2022
Institut de Biologie Moléculaire et Cellulaire
2018
IPO Porto
2018
Instituto de Biologia Molecular e Celular
2017
Universidade do Porto
2013-2017
Health First
2017
Universidade Católica Portuguesa
2015
Centro de Genética Clínica
2014
Instituto Nacional de Investigação Agrária e Veterinária
2013
Abstract Thymic epithelial cells (TECs) help orchestrate thymopoiesis, and TEC differentiation relies on bidirectional interactions with thymocytes. Although the molecular mediators that stimulate medullary thymic cell (mTEC) maturation are partially elucidated, signals regulate cortical (cTEC) homeostasis remain elusive. Using IL-7 reporter mice, we show TECs coexpressing high levels of (Il7YFP+ TECs) reside within a subset CD205+Ly51+CD40low cTECs coexpresses Dll4, Ccl25, Ccrl1, Ctsl,...
Cortical and medullary thymic epithelial cells (cTECs mTECs, respectively) provide inductive microenvironments for T‐cell development selection. The differentiation pathway of cTEC/mTEC lineages downstream common bipotent progenitors at discrete stages remains unresolved. Using IL‐7/CCRL1 dual reporter mice that identify specialized TEC subsets, we show the stepwise acquisition chemokine (C–C motif) receptor‐like 1 (CCRL1) is a late determinant cTEC differentiation. Although cTECs expressing...
Cortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T‐cell differentiation arise from cell progenitors (TEP). However, the nature of TEPs mechanism controlling their stemness in postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that fraction cTECs generates specialized clonal‐derived colonies, which contain with sustained colony‐forming capacity ( Clono TECs). These TECs are...
There is a great demand for the development of novel wound dressings to overcome time and costs care performed by vast number clinicians, especially in current overburdened healthcare systems. In this study, Cyanoflan, biopolymer secreted marine unicellular cyanobacterium, was evaluated as potential biomaterial healing. Cyanoflan effects on cell viability, apoptosis, migration were assessed vitro, while effect tissue regeneration biosafety healthy Wistar rats. The viability apoptosis...
Autoimmune regulator+ (Aire) medullary thymic epithelial cells (mTECs) play a critical role in tolerance induction. Several studies demonstrated that Aire+ mTECs differentiate further into Post-Aire cells. Yet, the identification of terminal stages mTEC maturation depends on unique fate-mapping mouse models. Herein, we resolve this limitation by segmenting mTEChi (MHCIIhi CD80hi ) compartment mTECA/hi (CD24- Sca1- ), mTECB/hi (CD24+ and mTECC/hi Sca1+ ). While included mostly Aire-expressing...
Abstract Despite the well-documented effect of castration in thymic regeneration, singular contribution bone marrow (BM) versus thymus to this process remains unclear. The chief role IL-7 pre- and intrathymic stages T lymphopoiesis led us investigate impact disrupting cytokine during rebound induced by androgen blockade. We found that promoted thymopoiesis young aged wild-type mice. In contrast, only germline IL-7–deficient (Il7−/−) mice consistently augmented after castration. increase cell...
Type 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed investigate how T2DM-associated characteristics (high levels glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence cancer carcinogenesis, in DMBA-treated (MCF-12ADMBA ) non-treated epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes proliferation, 3 H-DG uptake lactic acid production both The stimulatory effects insulin upon proliferation were hampered by rapamycin,...