A5018954750- Nitric Oxide and Endothelin Effects
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- T-cell and B-cell Immunology
- Multiple Myeloma Research and Treatments
- Photosynthetic Processes and Mechanisms
- Hormonal Regulation and Hypertension
- Macrophage Migration Inhibitory Factor
- Heat shock proteins research
- Virus-based gene therapy research
- Platelet Disorders and Treatments
- Protein Degradation and Inhibitors
- RNA Interference and Gene Delivery
- Electron Spin Resonance Studies
- Hemoglobin structure and function
- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- Advanced Fluorescence Microscopy Techniques
- Histone Deacetylase Inhibitors Research
- FOXO transcription factor regulation
- Protease and Inhibitor Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Liver physiology and pathology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
Centro de Investigaciones Biológicas Margarita Salas
2005-2023
Consejo Superior de Investigaciones Científicas
2005-2012
Centro Nacional de Biotecnología
2012
Universidad Complutense de Madrid
2003-2011
Universidad de Salamanca
2005
To explore the mechanisms by which NO elicits endothelial cell (EC) migration we used murine and bovine aortic ECs in an vitro wound-healing model. We found that exogenous or endogenous stimulated EC migration. Moreover, was significantly delayed derived from synthase-deficient mice compared with WT EC. assess contribution of matrix metalloproteinase (MMP)-13 to NO-mediated migration, RNA interference silence MMP-13 expression ECs. Migration cells silenced. In untreated localized caveolae,...
Recent data from multiple laboratories indicate that upon infection, many different families of viruses hijack the dynein motor machinery and become transported in a retrograde manner towards cell nucleus. In certain cases, one light chains, LC8, is involved this interaction. Using library overlapping dodecapeptides synthesized on cellulose membrane (pepscan technique) we have analyzed interaction chain LC8 with 17 polypeptides viral origin. We demonstrate strong binding two herpesvirus...
Cytoplasmic dynein is a large minus end-directed microtubule motor that translocates cargos towards the end of microtubules. Light chain 8 machinery (LC8) has been reported to interact with variety proteins possess K/RSTQT or GIQVD motifs in their sequence, hence permitting transport retrograde manner. Yeast two-hybrid analysis revealed brain, LC8 associates directly several such as neuronal nitric oxide synthase, guanylate kinase domain-associated protein and gephyrin. In this work, we...
Diacylglycerol kinase α (DGKα) regulates diacylglycerol levels, catalyzing its conversion into phosphatidic acid. The isoform is central to immune response regulation; it downmodulates Ras-dependent pathways and necessary for establishment of the unresponsive state termed anergy. DGKα functions are regulated in part at transcriptional level although mechanisms involved remain poorly understood. Here, we analyzed 5' end structure mouse gene detected three binding sites forkhead box O (FoxO)...
Abstract The interaction of multiple myeloma (MM) cells with the bone marrow (BM) microenvironment promotes MM cell retention, survival, and resistance to different anti‐MM agents, including proteasome inhibitors (PIs) such as bortezomib (BTZ). α4β1 integrin is a main adhesion receptor mediating cell–stroma interactions its expression function are downregulated by BTZ, leading inhibition adhesion‐mediated drug (CAM‐DR) apoptosis. Whether decreased activity maintained or recovered upon...
In this article we analyze the mechanisms by which C-terminal four amino acids of inducible nitric oxide synthase (NOS2) interact with proteins that contain PDZ (PSD-95/DLG/ZO-1) domains resulting in translocation NOS2 to cellular apical domain. It has been reported human hepatic associates EBP50, a protein two present epithelial cells. We describe herein binds through its carboxy-terminal residues CAP70, contains modules is targeted membranes. Interestingly, interaction augments both...
We have performed the recombinant expression and purification of reductase domain endothelial nitric oxide synthase (eNOS) used it as a bait in search for interacting proteins present cells. Using mass spectrometry bound run PAGE–SDS gel, we were able to identify ryanodine receptor (RyR) novel eNOS‐binding partner. This interaction was confirmed through immunoprecipitation both RyR eNOS from cells cardiac myocytes. Immunofluorescence data indicated that subpopulation associates with...
Human dynein light chain 1 (DYNLL1) is a dimeric 89‐residue protein that known to be involved in cargo binding within the multiprotein complex. Over 20 targets, of both cellular and viral origin, have been shown interact with DYNLL1, some them are transported retrograde manner along microtubules. Using DYNLL1 as bait yeast two‐hybrid screen human heart library, we identified GRINL1A (ionotropic glutamate receptor N ‐methyl‐ d ‐aspartate‐like 1A), homolog ionotropic ‐methyl ‐aspartate,...
The α4β1 integrin regulates the trafficking of multiple myeloma (MM) cells and contributes to MM disease progression. MicroRNAs (miRNAs) can have both tumor suppressor oncogenic roles thus are key controllers evolution, been associated with different phases pathogenesis. Using small RNAseq analysis, we show here that α4β1-dependent cell adhesion expression forty miRNAs, therefore expanding our current view involvement in biology. Specific upregulation miR-324-5p miR-331-3p attached ligands...