A5029823029- Genetics, Aging, and Longevity in Model Organisms
- Microfluidic and Bio-sensing Technologies
- Microbial Inactivation Methods
- Magnetic and Electromagnetic Effects
- thermodynamics and calorimetric analyses
- Circadian rhythm and melatonin
- Smart Agriculture and AI
- Heat shock proteins research
ETH Zurich
2021
Abstract Significant non‐genetic stochastic factors affect aging, causing lifespan differences among individuals, even those sharing the same genetic and environmental background. In Caenorhabditis elegans , in heat‐shock response (HSR) are predictive of lifespan. However, contributing to heterogeneity HSR still not fully elucidated. Here, authors characterized dynamics isogenic C. expressing GFP reporter for hsp‐16.2 identifying key contributors heterogeneity. Specifically, microfluidic...
Comprehensive integration of process steps into a miniaturised version synthetic biology workflows remains crucial task in automating the design biosystems. We present an acoustofluidic chip, capable automated medium exchange bacteria.
Abstract Genetics, environment, and stochasticity influence the rate of ageing in living organisms. Individual Caenorhabditis elegans that are genetically identical cultured same environment have different lifespans, suggesting a significant role ageing. We developed novel microfluidic methodology to measure heat-shock response as surrogate marker for heterogeneity associated with lifespan quantified C. at population, single individual, tissue levels. further mathematically modelled our data...
In article number 2102145, Collin Y. Ewald, Rudiyanto Gunawan, and co-workers highlight a microfluidic device used for sorting adult C. elegans populations—in high-throughput manner—based on individual GFP expression. The heterogeneous dynamic heat-shock response, its relation to proteostasis, how it can predict lifespan are thoroughly studied. Image credit: Mrs. Yujia Ma.
Transformation, i.e. reprogramming of bacteria by delivering exogenous genetic material (such as DNA) into the cytoplasm, is a key process in molecular engineering and modern biotechnology general. Transformation often performed electroporation, creating pores membrane using electric shocks low conductivity environment. However, cell preparation for electroporation can be cumbersome it requires exchange growth medium (high-conductivity) low-conductivity medium, typically via multiple...
Transformation, i.e. reprogramming of bacteria by delivering exogenous genetic material (such as DNA) into the cytoplasm, is a key process in molecular engineering and modern biotechnology general. Transformation often performed electroporation, creating pores membrane using electric shocks low conductivity environment. However, cell preparation for electroporation can be cumbersome it requires exchange growth medium (high-conductivity) low-conductivity medium, typically via multiple...