A5018827874- Insect Pest Control Strategies
- Insect Utilization and Effects
- Insect Resistance and Genetics
- Essential Oils and Antimicrobial Activity
- Biochemical and Structural Characterization
- Enzyme Production and Characterization
- Bioactive Compounds and Antitumor Agents
- Phytochemistry Medicinal Plant Applications
- Plant-Microbe Interactions and Immunity
- Glycosylation and Glycoproteins Research
- Papaya Research and Applications
- Moringa oleifera research and applications
- Aquaculture disease management and microbiota
- Botanical Research and Applications
- Transgenic Plants and Applications
- Ethnobotanical and Medicinal Plants Studies
- Mosquito-borne diseases and control
- Garlic and Onion Studies
- Pomegranate: compositions and health benefits
- Cassava research and cyanide
- Bacterial biofilms and quorum sensing
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Piperaceae Chemical and Biological Studies
Universidade Federal de Pernambuco
2012-2023
This work describes the isolation of a lectin (CasuL) from leaf pinnulae Calliandra surinamensis and evaluation its cytotoxic, antimicrobial antibiofilm properties. Proteins extract were precipitated with ammonium sulphate (60% saturation) submitted to Sephadex G-75 chromatography, which yielded isolated CasuL (purification factor: 113). Native is an acidic protein (pI 5.82) relative molecular mass 48 kDa. also oligomeric composed three subunits spectrometry revealed similarities Sorghum...
In this study, a leaf extract from Schinus terebinthifolius was evaluated for effects on survival, development, and midgut of A. aegypti fourth instar larvae (L4), as well toxic effect Artemia salina. Leaf obtained using 0.15 M NaCl phytochemical composition lectin activity. Early L4 were incubated with the (0.3–1.35%, w/v) 8 days, in presence or absence food. Polymeric proanthocyanidins, hydrolysable tannins, heterosid aglycone flavonoids, cinnamic acid derivatives, traces steroids,...
Schinus terebinthifolius leaves are used for treating human diseases caused by micro-organisms. This work reports the isolation, characterization and antimicrobial activity of S. leaf lectin (SteLL).The isolation procedure involved protein extraction with 0.15 mol l(-1) NaCl, filtration through activated charcoal chromatography filtrate on a chitin column. SteLL is 14-kDa glycopeptide haemagglutinating that inhibited N-acetyl-glucosamine, not affected ions (Ca(2+) Mg(2+)) stable upon heating...
The Alpinia purpurata inflorescence contains a lectin (ApuL), which has immunomodulatory activities on human cells. In the present work, it was evaluated antibacterial and antifungal effects of ApuL against pathogens. showed bacteriostatic activity non-resistant (UFPEDA-02) an oxacillin-resistant isolate (UFPEDA-672) Staphylococcus aureus with minimal inhibitory concentrations (MIC50) 50 400 μg/mL, respectively. addition, bactericidal effect (minimal concentration: 200 μg/mL). For Candida...
The plant Moringa oleifera is used as food and medicine. M. flowers are source of protein, fiber, antioxidants, to treat inflammation tumors. This work evaluated the antitumor activity flower trypsin inhibitor (MoFTI) in sarcoma 180-bearing mice. Swiss female mice were inoculated with 180 cells. Seven days later, animals treated intraperitoneally for 1 week daily doses PBS (control) or MoFTI (15 30 mg/kg). For toxicity assessment, water consumption, body organ weights, histological...
Tecoma stans (yellow elder) has shown medicinal properties and antimicrobial activity. Previous reports on antifungal activity of T. preparations presence trypsin inhibitor from leaves stimulated the investigation reported here. In this work, we proceeded to purification characterization a (TesTI), which was investigated for anti-Candida Finally, in order determine potential TesTI as new natural chemotherapeutic product, its cytotoxicity human peripheral blood mononuclear cells (PBMCs)...
In this study, we report the purification and characterization of a multifunctional lectin (MvFL) from Microgramma vacciniifolia fronds as well its immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). MvFL (pI 4.51; 54 kDa) is glycoprotein able to inhibit trypsin activity that has sequence similarities (32% coverage) with plant RNA-binding protein. Hemagglutinating was not altered by heating at 100 °C for 30 min, but reduced in alkaline pH (8.0 9.0). Fluorimetric...
In this study, the effects of Opuntia ficus-indica lectin (OfiL) on survival and nutritional parameters Sitophilus zeamais (maize weevil) adults were evaluated. OfiL was incorporated into artificial diets at concentrations 15, 60, 95 mg/g (mg per g wheat flour). Mortality evaluated after 7 15 days, amount food ingested weight insects determined 7th day. addition, in vitro gut enzymes insect investigated. The ingestion did not show any significant difference mortality rates compared to...
Abstract This work aimed to evaluate the effects of CasuL on growth and viability 15 mastitis isolates from cows goats, determine synergistic potential between antibiotics, investigate bacterial ultrastructure antibiofilm activity. The lectin inhibited Staphylococcus either bovine (Ssp6PD Sa) or caprine (Ssp5D Ssp01) mastitis. minimal inhibitory concentrations were ranged 3.75 µg/ml. Synergistic effect was observed for CasuL‐tetracycline against Sa Ssp6PD CasuL‐ampicillin Ssp01. No...
This work describes purification of a protease from the visceral mass mussel Mytella charruana as well evaluation its ability to hydrolyze milk casein generate antimicrobial peptides. The enzyme showed pI 4.1 and single polypeptide band 83.1 kDa after SDS-PAGE. Sequence similarities with tropomyosin myosin mollusks were detected. trypsin-like activity optimal temperature 40 °C stability in wide pH range (3.0-9.0). Km was 4.28 ± 0.34 mM synthetic substrate N-benzoyl-dl-arginyl-ρ-nitroanilide,...
The presence of color in textile effluents has been studied because the need for more effective treatments. Therefore, advanced oxidative processes (AOP) have used degradation dyes, as well conversion organic matter. This study evaluated direct orange 26 dye by Fenton and photo-Fenton (with natural solar radiation). A statistical analysis, based on factorial 23 indicated best working conditions, being: [H2O2] = 100 mg·L-1 pH 3-4, both AOP that [Fe] 1 e 5 mg·L-1, Fenton, respectively. results...
To evaluate the anti‐staphylococcal effects of lectins isolated from bark (MuBL), heartwood (MuHL) and leaves (MuLL) Myracrodruon urundeuva. The were evaluated for: on growth, aggregation, haemolytic activity biofilm‐forming ability Staphylococcus aureus clinical isolates nonresistant (8325‐4) multidrug resistant (LAC USA300); interference with expression virulence genes (hla, rnaIII spa) Agr system S. aureus; synergistic effect antibiotics cefoxitin cefotaxime. MuBL, MuHL MuLL reduced...
The objective of this study was to conduct phytochemical characterization and biological evaluation Hymenaea eriogyne. Crude extracts fractions from the bark, leaves pods, were obtained for screening by TLC HPLC, antibacterial antioxidant potential. Chromatographic data revealed presence several metabolites, notably flavonoid class. HPLC analysis confirmed flavanonol astilbin (taxifolin 3-O-ramnoside) other flavonoids derived aglycone taxifolin. In addition, it possible quantify markers in...
The chemical composition, the antioxidant and antimicrobial potential of crude extract from leaves Cinnamomum verum their enriched fractions was studied. Phytochemical analyses were performed by TLC HPLC, capacity verified DPPH• ABTS•+. Minimal Inhibitory/Bactericidal Concentration conducted against twenty-two bacteria to select five strains susceptible extracts/fractions resistant antibiotics tested. Interference Ethyl Acetate Fraction (EAF) in resistance synthetic antibiotic assayed...
Angiogenesis (budding of new blood vessels) is involved in several processes, including the development embryos and growth tumors. Schinus terebinthifolia leaves express an antitumor lectin (SteLL). This work hypothesized that SteLL can interfere with formation a vascular network from preexisting vessels. To test this hypothesis, effect on angiogenesis process was assessed using vivo model yolk sac membrane Coturnix japonica embryos. isolated purification factor 46.6. As expected,...
resumo
The aim of this study was to evaluate the phytochemical composition, antioxidant, and antimicrobial potential crude extract fractions Punica granatum leaves. produced by turbo extraction, after which hexanic, ethyl acetate, aqueous were obtained partitioning. chemical analyses performed thin layer chromatography high-performance liquid chromatography, antioxidant activities assayed DPPH. ABTS.+ . Minimal inhibitory bactericidal concentrations (MIC/MBC) applied twenty-two bacteria. Most...
This study evaluated the effects of acute exposure Aedes aegypti third instar (L3 ) larvae to saline extract Opuntia ficus-indica cladodes on biological cycle and fertility emerging adults. For this, were treated for 24 h with at ¼ LC50 (lethal concentration kill 50% larvae), ½ or ; development reproduction emerged adults after a recovery period 9 days. The resistance proteins in hydrolysis by L3 digestive enzymes histomorphological alterations larval midgut also investigated. contained...