A5010666247- Zebrafish Biomedical Research Applications
- Aquaculture disease management and microbiota
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- High Altitude and Hypoxia
- Genetic and phenotypic traits in livestock
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Phagocytosis and Immune Regulation
- Adipose Tissue and Metabolism
- Fish Ecology and Management Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Dendrimers and Hyperbranched Polymers
- Physiological and biochemical adaptations
- Bacterial Infections and Vaccines
University of Oslo
2020-2023
Université Claude Bernard Lyon 1
2014-2021
Biologie Tissulaire et Ingénierie Thérapeutique
2017-2021
Centre National de la Recherche Scientifique
2014
Institut de Biologie et de Chimie des Protéines
2014
The development of safe and effective vaccines for cancer infectious diseases remains a major goal in public health. Over the last two decades, controlled release vaccine antigens immunostimulant molecules has been achieved using nanometer or micron-sized delivery vehicles synthesized biodegradable polymers. In addition to achieving depot effect, enhanced efficacy such attributed efficient targeting antigen presenting cells as dendritic cells. Biodegradable biocompatible poly(lactic acid)...
Abstract The zebrafish embryo is a vertebrate well suited for visualizing nanoparticles at high resolution in live animals. Its optical transparency and genetic versatility allow noninvasive, real‐time observations of vascular flow their interactions with cells throughout the body. As consequence, this system enables acquisition quantitative data that are difficult to obtain rodents. Until now, few studies using model have only described semiquantitative results on key nanoparticle...
In bony fish, the gill filaments are essential for gas exchanges, but also vulnerable to infection by water-borne microorganisms. Omnipresent across gill-associated lymphoid tissues (GIALT) regulate interactions with local microbiota and halt pathogens. A special GIALT structure has recently been found in Salmonids, interbranchial tissue (ILT). However, structural variation of fish remains largely unknown. Here, we show how this critical zone interaction evolved fishes. By labeling a...
The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses be extremely important for their survival. Here, we used a marker T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques investigate lymphoid architecture zebrafish cavity. We identified sub-pharyngeal organ, which tentatively named “Nemausean organ” (NELO). NELO is enriched in T/NK cells, plasma/B antigen-presenting embedded network reticulated epithelial...
TLR ligands are critical activators of innate immunity and being developed as vaccine adjuvants. However, their usefulness in conjunction with NOD-like receptor agonists remains poorly studied. In this study, we evaluated a new ligand that targets both TLR2 NOD2 receptors. We assessed its ability to enhance dendritic cell maturation vitro addition improving systemic mucosal immune responses mice. The chimeric NOD2/TLR2 induced synergistic upregulation markers, costimulatory molecules,...
Activation of mucosal immunity is a key milestone for next-generation vaccine development. Biocompatible polymer-based nanoparticles (NPs) are promising vectors and adjuvants vaccination. However, their in vivo uptake by mucosae biodistribution antigen-presenting cells (APCs) need to be better understood optimize nanovaccine designs. Here, we assessed if APCs efficiently targeted spontaneous manner surfactant-free poly(lactic acid) (PLA-NPs) after administration. Combining histology flow...
The zebrafish is extensively used as an animal model for human and fish diseases. However, our understanding of the structural organization its immune system remains incomplete, especially mucosa-associated lymphoid tissues (MALTs). Teleost MALTs are commonly perceived diffuse scattered populations cells throughout mucosa. Yet, structured have been recently discovered in Atlantic salmon (Salmo salar L.), including interbranchial tissue (ILT) gills. existence ILT was only identified other...
BackgroundThe developing zebrafish is an emerging tool in nanomedicine, allowing non-invasive live imaging of the whole animal at higher resolution than possible more commonly used mouse models. In addition, several transgenic fish lines are available endowed with selected cell types expressing fluorescent proteins; this allows nanoparticles to be visualized together host cells.MethodsHere, we introduce neural tube as a robust injection site for cancer cells, excellently suited high imaging....
Nasal immunity is an ancient and conserved arm of the mucosal immune system in vertebrates. In teleost fish, we previously reported presence a nasopharynx-associated lymphoid tissue (NALT) characterized by scattered cells located trout olfactory lamellae. This diffuse NALT mounts innate adaptive responses to nasal infection or vaccination. mammals, structures such as adenoids tonsils support affinity maturation response nasopharyngeal cavity. These structures, known organized (O-NALT), have...
In the development of therapeutic nanoparticles (NP), there is a large gap between in vitro testing and vivo experimentation. Despite its prominence as model, mouse shows severe limitations for imaging NP cells with which they interact. Recently, transparent zebrafish larva, well suited high-resolution live-imaging, has emerged powerful alternative model to investigate behavior NP. Poly(D,L lactic acid) (PLA) widely accepted safe polymer prepare However, prevent aggregation, many require...
ABSTRACT The constant exposure of the fish branchial cavity to aquatic pathogens must have driven local mucosal immune responses be extremely important for their survival. In this study, we used a universal marker T lymphocytes/natural killer cells (ZAP70) and advanced imaging techniques investigate lymphoid architecture zebrafish cavity. We identified new organ, which tentatively named “Nemausean Lymphoid Organ” (NEMO), situated below pharynx, closely associated with gill tissues. Besides...
We report a profound upregulation of most egln paralog mRNA levels in anoxia and upon reoxygenation, with egln3ii showing the largest, 17-fold increase, highest relative abundance. The abundance prolyl hydroxylase domain (PHD) proteins was maintained during increased at reoxygenation. PHD immunoreactivity localized to axonal branches region-specific changes anoxia-reoxygenation. These dynamic regional crucian carp, champion tolerance, are likely adaptive call for further mechanistic studies.