A5043032669- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- HIV Research and Treatment
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- Nuclear Receptors and Signaling
- Chromosomal and Genetic Variations
- interferon and immune responses
- Apelin-related biomedical research
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- vaccines and immunoinformatics approaches
- RNA modifications and cancer
- Animal Disease Management and Epidemiology
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Multiple Sclerosis Research Studies
- RNA regulation and disease
- RNA and protein synthesis mechanisms
Aarhus University
2009-2022
Aarhus University Hospital
2016
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2006
Lambda Instruments (United States)
2001
Dalhousie University
2001
Abstract Stimulator of interferon genes (STING) is known be involved in control DNA viruses but has an unexplored role RNA viruses. During infection with STING activated downstream cGAMP synthase (cGAS) to induce type I interferon. Here we identify a STING-dependent, cGAS-independent pathway important for full production and antiviral enveloped viruses, including influenza A virus (IAV). Further, IAV interacts through its conserved hemagglutinin fusion peptide (FP). Interestingly, FP...
Autoimmune diseases encompass a plethora of conditions in which the immune system attacks its own tissue, identifying them as foreign. Multiple factors are thought to contribute development response self, including differences genotypes, hormonal milieu, and environmental factors. Viruses human endogenous retroviruses have long been linked occurrence autoimmunity, but never proven be causative Endogenous viruses retroviral sequences embedded host germline DNA transmitted vertically through...
Human endogenous retroviruses (HERVs) are remnants of past retroviral infections in the human genome and have been implicated different aspects biology. The aim this study was to identify HERVs that associated with pathogenesis rheumatic diseases such as systemic lupus erythematosus (SLE).The subjects included 45 female patients SLE 50 healthy controls matched for geographic area, age, sex. Real-time reverse transcription-polymerase chain reaction analysis used examine transcription levels...
We recently described that the autoimmune, central nervous system disease, multiple sclerosis (MS), is genetically associated with human endogenous retroviral locus, HERV-Fc1, in Scandinavians. A number of dominant genes encoding factors restrict retrovirus replication have been known for a long time. Today restriction retroviruses include amongst others TRIMs, APOBEC3s, BST2 and TREXs. therefore looked role these MS using genetic epidemiology. here report markers two TRIM5 TRIM22 marker...
ABSTRACT SL3-2 is a polytropic murine leukemia virus with limited species tropism. We cloned the envelope gene of this virus, inserted it into bicistronic vector, and found that protein differs from other, similar proteins also utilize receptor (Xpr1) in severely impaired mediating infection human mink cells. two adjacent amino acid mutations (G212R I213T), located previously functionally uncharacterized segment surface subunit, are responsible for restricted tropism wild-type envelope. By...
Short dimeric or mulitmeric peptides derived from a highly conserved stretch of amino acids gammaretroviral envelope proteins has been found to have immunosuppressive properties in vitro. Here we test the hypothesis that such may serve as immunomodulatory reagents for treatment inflammatory disorders. The anti-inflammatory effect synthetic retrovirus-derived peptide 17 was tested two murine skin inflammation models, TPA-induced acute toxic contact eczema model and an oxazolone-induced...
Abstract This study evaluates the immunogenicity of HIV envelope protein (env) in mice presented either attached to γ-retroviral virus-like-particles (VLPs), associated with cell-derived microsomes or as solubilized recombinant (gp160). The magnitude and polyfunctionality cellular immune response was enhanced when delivering env VLP microsome form compared gp160. Humoral responses measured by antibody titres were comparable across groups low levels neutralization observed. Lastly, we...
Background Detection of a novel Gammaretrovirus, Xenotropic Murine leukaemia virus-Related Virus (XMRV) has been reported in peripheral blood mononuclear cells patients with chronic fatigue syndrome, and prostate cancer tissue. As Multiple Sclerosis (MS) is disease retroviral association (human endogenous retroviruses (HERVs)), we investigated whether XMRV could be contributing to MS aetiology by testing well defined cohort Danish for the presence sequences.
We have constructed a replication-competent gammaretrovirus (SL3-AP) capable of using the human G-protein-coupled receptor hAPJ as its entry receptor. The envelope protein virus was made by insertion 13-amino-acid peptide ligand for hAPJ, flanked linker sequences, into one variable loops binding domain SL3-2, murine leukemia (MLV) that uses xenotropic-polytropic Xpr1 and which has host range limited to cells. This can utilize well cells with equal efficiencies. In addition, SL3-AP replicates...
<h3>Background</h3> Human Endogenous Retroviruses (HERVs) are ancient retroviral integrations fixed in the genome of humans. As for all retroviruses, HERVs contain three protein coding genes: <i>gag</i>, <i>pol</i> and <i>env.</i> The envelope proteins can influence human biology through their inherent immune suppressive activity (1) localized to a secondary structure (the ISU domain) protein. Our previous research has shown that env-59-protein HERV-H is negatively correlated inflammatory...
ABSTRACT We have previously reported the construction of a murine leukemia virus-based replication-competent gammaretrovirus (SL3-AP) capable utilizing human G protein-coupled receptor APJ (hAPJ) as its entry and natural receptor, Xpr1 with equal affinities. The apelin has been shown to function coreceptor for HIV-1, thus, adaptation viral vector this is significant interest. Here, we report molecular evolution SL3-AP envelope protein when virus cultured in cells harboring either or hAPJ...
Abstract Background SL3-2 is a unique polytropic murine gammaretroviral isolate that only able to infect cells. We have previously shown two mutations R212G and T213I located on the surface of receptor binding domain in region designated VR3 loop can alter species tropism this envelope protein. This location suggests composition has an influence interaction thereby affects as well superinfection resistance. In order investigate further, we studied interference patterns its mutants. Results...