- Mollusks and Parasites Studies
- Aquatic Invertebrate Ecology and Behavior
- Marine Biology and Ecology Research
- Invertebrate Taxonomy and Ecology
- Animal Ecology and Behavior Studies
- Genetic diversity and population structure
- Evolution and Paleontology Studies
- Fish Ecology and Management Studies
- Study of Mite Species
- Wildlife Ecology and Conservation
- Chromium effects and bioremediation
- Fossil Insects in Amber
- Parasite Biology and Host Interactions
- Paleopathology and ancient diseases
- Freshwater macroinvertebrate diversity and ecology
- Helminth infection and control
- Marine Bivalve and Aquaculture Studies
- Amphibian and Reptile Biology
- Non-Invasive Vital Sign Monitoring
- Minimally Invasive Surgical Techniques
- Ichthyology and Marine Biology
- Marine and coastal plant biology
- Ancient Near East History
- Spectroscopy and Chemometric Analyses
- Dermatological and COVID-19 studies
University of the Ryukyus
2023-2024
Tohoku University
1996-2023
University of Tsukuba
2022
Toho University
2021
University of Idaho
2019
University of Sheffield
2017
Australian National University
2017
Université de Montpellier
2017
Centre National de la Recherche Scientifique
2017
University of St Andrews
2017
We examined the biological function of a nonstructural regulatory protein, NS1, human parvovirus B19. Because cytotoxic activity hematopoietic cell lines, K562, Raji, and THP-1, were established as transfectants which produce viral NS1 protein upon induction by using bacterial lactose repressor/operator system. was significantly produced in three transfectant cells an inducer dose- time-dependent manner. Surprisingly, these secreted inflammatory cytokine, interleukin-6 (IL-6), response to...
Phylogenetic incongruence has frequently been encountered among different molecular markers. Recent progress in phylogenomics provided detailed and important information for evolutionary biology taxonomy. Here we focused on the freshwater viviparid snails (Cipangopaludina chinensis C. c. laeta) of East Asia. We conducted phylogenetic analyses divergence time estimation using two mitochondrial also performed population genetic genome-wide SNPs. investigated how which patterns reflect shell...
Islands have traditionally been the centre of evolutionary biological research, but dynamics immigration and differentiation at continental islands not well studied. Therefore, we focused on Japanese archipelago, located eastern end Eurasian continent. While archipelago is characterised by high biodiversity rich freshwater habitats, origin formation mechanisms its organisms are clear. In order to clarify history planorbid gastropod fauna, conducted phylogenetic analysis, divergence time...
We document extensive incongruence between molecular phylogeny and the current taxonomy of freshwater gastropod family Viviparidae in Japan. A based on mitochondrial cytochrome oxidase subunit I 16S rRNA genes for East Asian viviparids comprises three major clades, two largest which are composed primarily paraphyletic or highly polyphyletic taxonomic species. Sinotaia quadrata histrica includes several lineages whose geographical distribution shell morphology largely incongruent with their...
Abstract Endemic organisms of ancient lakes have been studied as models to understand processes speciation and adaptive radiation. However, it remains unclear how play roles in genetic phenotypic diversity freshwater mollusks. In the present study, we focus on viviparid snails East Southeast Asia (Japan China) address this question. Using molecular phylogenetic analyses based mitochondrial (COI, 16S) nuclear genes (18S, 28S, H3), show that patterns species diversification lineages....
Abstract Other than hard bones and shells, it is rare for soft tissues to fossilize, but occasionally they are well-preserved in amber. Here, we focus on both modern fossilized species of the land snail superfamily Cyclophoroidea. Phylogenetic relationships within Cyclophoroidea were previously studied using extant species, timing divergence group remains unclear. In addition, difficult observe morphological traits such as chitinous operculum periostracum fossil snails due their poor...
The Japanese Archipelago harbours high diversity of endemic bradybaenid land snails. However, there have been few systematic studies these resolution the taxonomy and phylogenetic relationships snail taxa is important both for describing species promoting conservation We investigated molecular phylogeny Bradybaena Phaeohelix using CO1 internal transcribed spacer genes, to clarify whether morphological traits current genera reflect their relationships. Our results show that in are genetically...
Abstract The Holarctic land snail genus Perpolita was used to explore the influence of past and current biogeography on diversification. number empirically-supported species determined using a consensus between mtDNA sequence, nDNA conchology, geographic ecological range with five valid temperate-boreal (Perpolita binneyana, electrina, hammonis, petronella, radiatella) being recognized. Only P. petronella unchanged in both nomenclature diagnostic characteristics remainder requiring...
The role of natural selection in phenotypic evolution is central to evolutionary biology. Phenotypic affected by various factors other than adaptation, and recent focus has been placed on the effects phylogenetic constraints niche conservatism evolution. Here, we investigate relationship between shell morphology habitat use bradybaenid land snails genus Aegista clarify causes divergence among phylogenetically related species. results ancestral state reconstruction showed that arboreal...
Abstract Variation in the shell coiling, or chirality, of land snails provides an opportunity to investigate potential for “single-gene” speciation, because mating between individuals opposite chirality is believed not possible if mate a face-to-face position. However, evidence support single-gene speciation sparse, mostly based upon mitochondrial studies and patterns chiral variation species. Previously, we used theoretical model show that as phenotype offspring determined by maternal...
Abstract Aim Vicariance events have been proposed as a major source of lineage divergence on continental islands, whereas dispersal followed by isolation the cause oceanic islands. However, organisms islands may include taxa with characteristics similar to those Lineage unassociated geological that separated also occurred. This study addresses these possibilities through morphological and molecular phylogeographic analyses land snails ( Bradybaena ) Location Samples were mainly collected...
Abstract To clarify the effect of niche conservatism on evolutionary history, we focused freshwater snails, which have different ecological and phylogenetic properties from previously tested taxa. We conducted a analysis using 750 lymnaeid individuals 357 sites eleven Radix species. Then, estimated ancestral distribution geographic coordinates colonization routes. In addition, statistical test distances in latitudinal longitudinal directions was performed. also modeling for two widely...
Confirming the extinction of species with only old occurrence records is often difficult, due to scarcity biological information. Phylogenetic position and taxonomic status potentially extinct are unclear in most such cases. In present study, we document that a endemic genus Hirasea oceanic Ogasawara Islands, which was formerly believed have become extinct, still survives, on basis combined surveys involving molecular phylogenetics morphometrics. notorious for its highly diversified shell...
Abstract The insular limestone karsts of northern Vietnam harbor a very rich biodiversity. Many taxa are strongly associated with these environments, and individual species communities can differ considerably among karst areas. exact processes that have shaped the biotic composition habitats, however, remain largely unknown. In this study, role two major for assembly snail on was investigated, interspecific competition filtering due to geographical factors. Communities operculate land snails...
Limestone karsts can form terrestrial habitat islands for calcium-dependent organisms. In Vietnam, many karst habitats are threatened, while their rich biodiversity is still far from being thoroughly explored. Given that conservation of biota strongly relies on correct species identification, the presence undetected cryptic pose severe problems. The present study focuses diversity among karst-inhabiting land snails genus Cyclophorus in northern where specimens with a similar shell morphology...
Abstract Background Anthropogenic factors potentially affect observed biogeographical patterns in population genetics, but the effects of ancient human activities on original created by natural processes are unknown. Sinotaia quadrata , a widely distributed freshwater snail species East Asia, was used to investigate this issue. It is unclear whether S. Japan introduced from China and how different uses varying geographic contemporary genetics between two regions. Thus, we investigated...