A5024262596- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune cells in cancer
- Cancer Cells and Metastasis
- T-cell and B-cell Immunology
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- CAR-T cell therapy research
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Cervical Cancer and HPV Research
- Plant Virus Research Studies
- Gut microbiota and health
- Chemokine receptors and signaling
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Skin and Cellular Biology Research
- Immune Response and Inflammation
- Plant Molecular Biology Research
- Biofuel production and bioconversion
- Cytokine Signaling Pathways and Interactions
University of Cambridge
2020-2025
Babraham Institute
2020-2024
Chiayi Chang Gung Memorial Hospital
2021-2023
The University of Queensland
2016-2021
Translational Research Institute
2016-2021
Institute of Molecular Biology, Academia Sinica
1996-2016
National Yang Ming Chiao Tung University
2007-2016
Academia Sinica
1969-2010
The University of Texas Medical Branch at Galveston
1997
Institute of Botany
1971-1996
The gut microbiome modulates immunotherapy treatment responses, and this may explain why immune checkpoint inhibitors, such as anti-PD-1, are only effective in some patients. Previous studies correlated lipopolysaccharide (LPS)-producing microbes with poorer prognosis; however, LPS from diverse bacterial species can range immunostimulatory to inhibitory. Here, by functionally analysing faecal metagenomes 112 patients melanoma, we found that a subset of LPS-producing bacteria encoding...
Abstract CD4 + regulatory T (Treg) cells, dependent upon the transcription factor Foxp3, contribute to tumour immunosuppression but are also required for immune homeostasis. There is interest in developing therapies that selectively target immunosuppressive function of Treg cells within tumours without disrupting their systemic anti‐inflammatory function. High levels expression chemokine (C‐C motif) receptor 8 (CCR8) discriminate from those found lymphoid tissues. It has recently been...
Regulatory T (Treg) cell populations are composed of functionally quiescent resting Treg (rTreg) cells which differentiate into activated (aTreg) upon antigen stimulation. How rTreg remain despite chronic exposure to cognate self- and foreign antigens is unclear. The transcription factor BACH2 critical for early lineage specification, but its function following commitment unresolved. Here, we show that repurposed promotes the quiescence long-term maintenance cells. Bach2 highly expressed in...
// Erh-Hsuan Lin 1, 2, 3, 4 , Yu-Rung Kao 1 Chih-An 5 Ting-Yu Kuo Sheng-Ping Yang 2 Chiung-Fang Hsu Teh-Ying Chou 6 Chao-Chi Ho 7 Cheng-Wen Wu Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan Microbiology and Immunology, National Ming University, 3 Clinical Medicine, Yang-Ming Biochemistry Molecular Biology, Department Laboratory Sciences Medical Biotechnology, University College, Pathology Taipei Veterans General Hospital, Internal Hospital Correspondence to: Wu, e-mail:...
We examined the full-length hepatitis B virus (HBV) envelope (surface antigen or HBV small surface [HBsAg]) sequences of 12 different liver samples from 10 hepatoma-containing chronic carriers. Surprisingly, novel and frequent mutations occurred predominantly at amino acids 40 47 HBsAg, in addition to within a known protective B-cell epitope (so-called group determinant HBsAg 124-148). Approximately 58% carriers contain determinant. The mutation frequency hotspot codons is approximately 83%,...
Abstract Calcium/calmodulin‐dependent serine kinase (CASK), a causative gene in X‐linked mental retardation, carries out multiple functions neurons, including vesicle trafficking of ion channels, synapse formation, and transcription. From yeast two‐hybrid screen, Krüppel‐like zinc finger protein B cell lymphoma/COUP‐TF‐interacting 1 (Bcl11A/CTIP1) was identified as CASK binding protein. Through alternative splicing, single Bcl11A encodes two major products Bcl11A‐S Bcl11A‐L. interacted with...
A validated animal model would assist with research on the immunological consequences of chronic expression stress keratins KRT6, KRT16, and KRT17, as observed in human pre-malignant hyperproliferative epithelium. Here we examine keratin gene profile skin from mice expressing E7 oncoprotein HPV16 (K14E7) demonstrating persistently epithelium, nontransgenic mouse skin, actinic keratosis lesions skin. We demonstrate that K14E7 overexpresses a similar manner to keratoses, overexpression is...
Prophylactic human papillomavirus (HPV) vaccines are commercially available for prevention of infection with cancerogenic HPV genotypes but not able to combat pre-existing HPV-associated disease. In this study, we designed a nanomaterial-based therapeutic vaccine, comprising manganese (Mn4+)-doped silica nanoparticles (Mn4+-SNPs) and the viral neoantigen peptide GF001 derived from HPV16 E7 oncoprotein. We show in mice that Mn4+-SNPs act as self-adjuvants by activating inflammatory signaling...
Natural killer (NK) cells are critical to immune surveillance against infections and cancer. Their role in requires that NK present within tissues a quiescent state. Mechanisms by which remain incompletely elucidated. The transcriptional repressor BACH2 plays the adaptive system, but its function innate lymphocytes has been unclear. Here, we show acts as an intrinsic negative regulator of cell maturation function. is expressed developing mature promotes maintenance immature restricting their...
<p>Supplementary Figure 2: T cell populations in the spleen and tumor of FS120m treated mice. (A) Percentages indicated tumors mice with or control antibodies. (B) spleens (C) Absolute counts exTreg cells Foxp3+ RFP+ Treg per gram (left) whole (right) animals. (D) IFN-γ producing FS120m-treated (E) TNF * P ≤ 0.05, ** 0.01. Student’s t test. Bars error are mean s.e.m.</p>
<p>Supplementary Figure 3: Increased IL-2 expression by conventional CD4+ and CD8+ T cells with FS120m treatment. Representative plots (left) replicate measurements (right) of IL-2+ Tconv in the spleen draining lymph nodes on day 18 post tumor implantation. *** P ≤ 0.001, **** 0.0001. Unpaired Student’s t test. Bars error are mean s.e.m.</p>
<p>Supplementary Figure 1: OX40 and CD137 are highly expressed on Treg cells. Representative plots showing OX40+ (left) CD137+ (right) SP resting or activated cells from the spleen tumor day 18 post implantation. ** P ≤ 0.01, *** 0.001, **** 0.0001. One-way ANOVA with Tukey’s correction for multiple comparisons. Bars error mean s.e.m.</p>
Batf3 is a transcription factor that impacts the development of CD103 + tissue‐resident dendritic cells (DCs). However, whether absolutely required for CD8 DCs remains controversial. Id2 DC development. Here we show bone marrow chimeric mice with deletion in CD11c compartment lose ability to reject skin graft expressing non‐self protein antigen or mount delayed hypersensitivity response. In contrast, −/− remained competent rejection and hypersensitivity, retained population markers...
While neuronal activity regulates neurite outgrowth and branching, the details of underlying mechanisms are still largely unclear. This study investigated effect glutamate receptors on neuritogenesis using cultured hippocampal neurons. At 3-4 days in vitro, NMDA treatment promoted axon branching but not primary extension. In contrast, blockade receptor (NMDAR) by AP5 enhanced NMDAR activation also increased dendrite number total length. These results suggest that controls morphogenesis. A...
SUMMARY We have isolated a new filamentous phage from Xanthomonas citri, pathogenic bacterium of citrus canker. This phage, designated as Cf, measures 10075 ± 985 × 10−1 nm in length and forms an unusually clear but tiny plaque on the host within 12 h p.i. Infection by Cf neither killed nor drastically prevented cells propagation. However, rate cell division was severely retarded. exhibited exceedingly narrow range unstable conventional tris buffer synthetic medium routinely used for...
Certain factors which affect production and accumulation of indole-3- acetic acid (IAA) activity enzymes associated with its synthesis from L-tryptophan were investigated in Pseudomonas savastanoi. Addition L- tryptophan to the culture medium enhances IAA by bacterium. However, both oxidative decarboxylase indoleacetamide hydrolase, are involved conversion IAA, produced bacterium without addition medium. In a containing L-tryptophan, yields per liter suspension increased as much 2-fold.IAA...
It was demonstrated for the first time that filamentous bacteriophage Cflt, which contains single-stranded DNA, can incorporate its genome into of host. Evidence in support incorporation obtained from a Southern blot hybridization analysis DNA isolated Cflt-lysogenized cells. DNAs different cells were purified, and integration patterns compared. Because all identical only one fragment Cflt replicative-form missing, it appears site specific. Only complement viral integrated per host...
Data regarding the prevalence of depression and anxiety among cancer patients, especially before diagnosis, remains scarce. This study investigated these conditions associated drug use patients pre- post-diagnosis.This population-based cohort using data from Taiwan's National Health Insurance Research Database recruited with a registered diagnosis matched control between January 1, 2000, December 31, 2011. We compared depressive disorders non-cancer participants during 2-year period both...
Rejuvenation, as evidenced by restored rooting competence, of Sequoia sempervirens a series repeated grafting adult shoot tips onto juvenile rootstocks in vitro was confirmed. Complete restoration competence achieved after repeatedly five times. The rejuvenation correlated with disappearance adult-associated esterase and peroxidase isozymes an appearance isoesterases isoperoxidases that were characteristic juvenile-phase shoots. These could serve markers to assist phasechange investigations.
Abstract Bcl11A/Evi9/CTIP1, a Kruppel‐like zinc finger gene, plays an important role in B‐cell development. In addition to expression B lymphocytes, Bcl11A/Evi9/CTIP1 is also highly expressed the brain, although its function there still unclear. present study, regional and subcellular distributions of rat brain were investigated by immunostaining biochemical fractionation. Using antibodies recognizing first 18 amino acid residues distribution 2 isoforms gene products, Bcl11A‐L/Evi9a...
Abstract Immune checkpoint inhibitors (ICI), such as anti-PD-1, have revolutionized cancer treatment, but they are only effective for a minority of patients. The gut microbiome plays crucial role in modulating immunotherapy treatment responses, and previous studies correlated lipopolysaccharide (LPS)-producing microbes with poorer prognosis. However, LPS from diverse bacterial species activities ranging immunostimulatory to inhibitory. By functionally analyzing fecal metagenomes 112 melanoma...