A5020988596- Genetics, Bioinformatics, and Biomedical Research
- Academic Publishing and Open Access
- Scientific Computing and Data Management
- Biomedical Text Mining and Ontologies
- Research Data Management Practices
- Peroxisome Proliferator-Activated Receptors
- Bioinformatics and Genomic Networks
- Biosimilars and Bioanalytical Methods
- Biomedical Ethics and Regulation
- scientometrics and bibliometrics research
- Neuroscience and Neuropharmacology Research
- Gene Regulatory Network Analysis
- Mitochondrial Function and Pathology
- Microbial Community Ecology and Physiology
- Neuroendocrine regulation and behavior
- Data Quality and Management
- Estrogen and related hormone effects
- Receptor Mechanisms and Signaling
- Molecular Biology Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Academic integrity and plagiarism
- Circadian rhythm and melatonin
- Nuclear Receptors and Signaling
- Single-cell and spatial transcriptomics
- RNA regulation and disease
European Molecular Biology Organization
2015-2025
SIB Swiss Institute of Bioinformatics
2016
Meyer (China)
2012-2013
John Wiley & Sons (United Kingdom)
2012-2013
German Cancer Research Center
2001-2009
Heidelberg University
2002-2008
The Ohio State University
2008
Institute of Molecular and Cell Biology
2007
DKFZ-ZMBH Alliance
2001-2005
University of Freiburg
2005
Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone that can be activated by fatty acids and peroxisome proliferators. The PPARα subtype mediates the pleiotropic effects of these activators in liver regulates several target genes involved acid catabolism. In primary hepatocytes cultured vitro, gene is regulated at transcriptional level glucocorticoids. We investigated if this hormonal regulation also occurs whole animal physiological situations leading to increased plasma...
We describe the generation of transgenic mouse lines expressing Cre recombinase enzyme in brain under control CamKIIalpha gene present a BAC expression vector. The transgene gave faithful pattern resembling endogenous gene. Specifically, high levels were detected hippocampus, cortex, and amygdala, lower striatum, thalamus, hypothalamus. As expected, no was cerebellum or outside brain. level driven shown to be copy number dependent. To test activity recombinase, mice crossed with harbouring...
This study demonstrates that the expression of peroxisome proliferator-activated receptor alpha (PPAR alpha) is regulated by glucocorticoid hormones in hepatocytes. Hydrocortisone, dexamethasone, and triamcinolone stimulated PPAR mRNA synthesis a dose-dependent manner primary rat hepatocyte cultures. stimulation was inhibited RU 486, specific antagonist. Moreover, contrast to hormones, mineralocorticoid aldosterone had only weak effect, suggesting hormonal mediated receptor. The induction...
Marijuana and its main psychotropic ingredient Δ9-tetrahydrocannabinol (THC) exert a plethora of psychoactive effects through the activation neuronal cannabinoid receptor type 1 (CB1), which is expressed by different subpopulations in central nervous system. The exact neuroanatomical substrates underlying each effect THC are, however, not known. We tested locomotor, hypothermic, analgesic, cataleptic conditional knockout mouse lines, lack expression CB1 subpopulations, including principal...
Abstract This article presents a practical roadmap for scholarly publishers to implement data citation in accordance with the Joint Declaration of Data Citation Principles (JDDCP), synopsis and harmonization recommendations major science policy bodies. It was developed by Publishers Early Adopters Expert Group as part Implementation Pilot (DCIP) project, an initiative FORCE11.org NIH BioCADDIE program. The structure presented here follows “life paper” workflow includes categories...
Peer review is an important part of the scientific process, but traditional peer at journals coming under increased scrutiny for its inefficiency and lack transparency. As preprints become more widely used accepted, they raise possibility rethinking peer-review process. Preprints are enabling new forms that have potential to be thorough, inclusive, collegial than journal review, thus fundamentally shift culture toward constructive collaboration. In this Consensus View, we make a call action...
The peroxisome proliferator-activated receptors (PPAR) and thyroid hormone (TR) are members of the nuclear receptor superfamily, which regulate lipid metabolism tissue differentiation. In order to bind DNA activate transcription, PPAR requires formation heterodimers with retinoid X (RXR). addition activating transcription through its own response elements, is able selectively down-regulate transcriptional activity TR, but not vitamin D receptor. molecular basis this functional interaction...
The central nervous system is fundamentally dependent on guided cell migration, both during development and in adulthood. We report an absolute requirement of the transcription factor serum response (SRF) for neuronal migration mouse forebrain. Conditional, late-prenatal deletion Srf causes neurons to accumulate ectopically at subventricular zone (SVZ), a prime neurogenic region brain. SRF-deficient cells SVZ exhibit impaired tangential chain along rostral migratory stream into olfactory...
Induction of specific gene expression patterns in response to activity confers functional plasticity neurons. A principal role the regulation these processes has been ascribed cAMP responsive element binding protein (CREB). Using genome-wide profiling mice lacking CREB forebrain, accompanied by deletion modulator (CREM), we here show that proteins activity-induced is surprisingly selective and highly context dependent. Thus, only a very restricted subset genes (i.e., Gadd45b or Nr4a2)...
Abstract Background Dopamine-activated signaling regulates locomotor and emotional responses alterations in dopamine-signaling are responsible of several psychomotor disorders. In order to identify specific functions these pathways, the Cre/loxP system has been used. Here, we describe generation characterization a transgenic mouse line expressing Cre recombinase dopaminoceptive neurons. To this purpose, used as expression vector 140 kb yeast artificial chromosome (YAC) containing dopamine D1...
Text mining in the biomedical sciences is rapidly transitioning from small-scale evaluation to large-scale application. In this article, we argue that text-mining technologies have become essential tools real-world research. We describe four large scale applications of text mining, as showcased during a recent panel discussion at BioCreative V Challenge Workshop. draw on these case studies characterize common requirements for successfully applying techniques practical biocuration needs. note...
Preprints enable new forms of peer review that have the potential to be more thorough, inclusive, and collegial. In December 2022, 80 researchers representatives funders, institutions, preprint servers, journals, indexers, services were invited gather online at Janelia Research Campus for a workshop on Recognizing Preprint Peer Review. Sponsored by HHMI, ASAPbio, EMBO, this meeting aimed catalyze community consensus support create model funder, institutional, journal policies recognize both...
Here we describe the generation of a new tamoxifen-inducible double Cre fusion protein generated by fusing two ERT2 domains onto both ends iCre recombinase (a codon improved recombinase). This (ERiCreER) had twofold increased activity in cell culture assays than previously described MerCreMer protein. ERiCreER was targeted to brain placing it under control promoter from CamKIIalpha gene using 170 kb BAC. The detected hippocampus, cortex, striatum, thalamus, and hypothalamus but not...
Current methods to analyze gene expression measure steady-state levels of mRNA. To specifically mRNA transcription, we have developed a technique that can be applied in vivo intact cells and animals. Our method makes use the cellular pyrimidine salvage pathway is based on affinity-chromatographic isolation thiolated When combined with data levels, this able assess relative contributions synthesis degradation/stabilization. It overcomes limitations associated currently available such as...
Preprints catalyze rapid and open communication of research. A frequent criticism preprints, however, is their lack peer review. In recent years, myriad new initiatives have enabled review preprinted research to be coordinated, collected, displayed alongside preprints. This provides evaluation context for readers, as well feedback the authors. The processes behind preprint are diverse may differ from journal review, which can a challenge readers seeking compare interpret reviews. To address...
The Cre-loxP system is increasingly exploited for spatial and temporal gene inactivation. Here we present a novel approach to achieve this goal of selective Following the model alpha complementation in beta-galactosidase enzyme, where enzyme split into independent polypeptides which are able associate maintain enzymatic activity, divided Cre recombinase two (one containing NH(2) terminus (alpha) second one COOH-terminus (beta)). Individually, have no detectable activity. However, when...