A5027276778- Bacillus and Francisella bacterial research
- Enzyme Structure and Function
- Heat shock proteins research
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Vibrio bacteria research studies
- Microbial Inactivation Methods
- Plant biochemistry and biosynthesis
- Meat and Animal Product Quality
- Probiotics and Fermented Foods
- Environmental Toxicology and Ecotoxicology
- Risk and Safety Analysis
- Antibiotic Resistance in Bacteria
- Safety Warnings and Signage
- Listeria monocytogenes in Food Safety
- Pesticide and Herbicide Environmental Studies
- Fermentation and Sensory Analysis
- Microbial Metabolism and Applications
- Polyamine Metabolism and Applications
- Insect and Pesticide Research
- Pharmacological Effects of Natural Compounds
- Microbial Metabolic Engineering and Bioproduction
Sécurité et Qualité des Produits d'Origine Végétale
1998-2018
Université d'Avignon et des Pays de Vaucluse
2002-2015
Université de Bourgogne
2001
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
1997-1999
Institut National de la Recherche Agronomique
1999
In Leuconostoc oenos, different stresses such as heat, ethanol, and acid shocks dramatically induce the expression of an 18-kDa small heat shock protein called Lo 18. The corresponding gene (hsp18) was cloned from a genomic library L. oenos constructed in Escherichia coli. A 2.3-kb DNA fragment carrying hsp18 sequenced. encodes polypeptide 148 amino acids with calculated molecular mass 16,938 Da. Lo18 has significant identity proteins alpha-crystallin family. transcriptional start site...
In Leuconostoc oenos, a malolactic bacterium, the synthesis of stress protein called LO18 with an apparent molecular mass 18 kDa was greatly induced after heat (42 degrees C), acid (pH 3) or ethanolic (12% (v/v)) shocks. Moreover, in stationary growth phase and detected for long time (30 h) during this phase. Significant identity found between N-terminal parts Hsp18 from Clostridium acetobutylicum suggesting that belongs to family small shock proteins conserved prokaryotic eukaryotic cells.
Sulfite is an antimicrobial agent used at the beginning of winemaking to avoid development undesirable microorganisms. However, Oenococcus oeni, which mainly responsible for malolactic fermentation, has grow in wine and therefore be resistant sulfite. This study showed that acid-adapted cells O. oeni survived better than non-adapted presence a high sulfite concentration (30 mg l−1). Addition sub-lethal (15 l−1) during adaptation step acidic medium increases tolerance. Moreover, appeared able...
Sequencing of the DNA region located upstream alpha-acetolactate synthase and decarboxylase (alsS-alsD) cluster Oenococcus oeni allowed identification an ORF, named trxA. This encodes a protein 104 amino acids very similar to known thioredoxins. The encoded by cloned fragment was able complement Escherichia coli strains lacking functional thioredoxin. Considering results sequence comparisons complementation experiments, it concluded that trxA gene Studies expression showed abundance mRNA...
ABSTRACT Using degenerated primers from conserved regions of previously studied clpX gene products, we cloned the malolactic bacterium Oenococcus oeni . The was sequenced, and deduced protein 413 amino acids (predicted molecular mass 45,650 Da) highly similar to analyzed products other organisms. An open reading frame located upstream identified as tig by similarity its predicted product bacterial trigger factors. ClpX purified using a maltose binding fusion system shown possess an ATPase...
Journal Article Improved acid tolerance of a recombinant strain Escherichia coli expressing genes from the acidophilic bacterium Oenococcus oeni Get access F. Morel, Morel Laboratoire de Microbiologie, Université Bourgogne, Dijon, France Search for other works by this author on: Oxford Academic Google Scholar Delmas, Delmas M.‐P. Jobin, Jobin C. Diviès, Diviès J. Guzzo Dr Guzzo, UMR INRA, E.N.S.B.A.N.A., 1, esplanade Erasme, F‐21000 (e‐mail: jguzzo@u‐bourgogne.fr). Letters in Applied...
Abstract This study examined the involvement of ATP ase activity in acid tolerance response ( ATR ) Bacillus cereus ATCC 14579 strain. In current work, B. cells were grown anaerobic chemostat culture at external pH e 7.0 or 5.5 and a growth rate 0.2 h −1 . Population reduction internal i after shock 4.0 was either with without inhibitor N,N’ ‐dicyclohexylcarbodiimide DCCD ionophores valinomycin nigericin. strongly limited (acid‐adapted cells) compared (unadapted cells), indicating that low...
Characterization of small heat shock proteins in lactic acid bacteria.Among (Hsp), low molecular weight Hsp (smHsp) are the less known.Four smHsp were recently studied bacteria (Streptococcus thermophilus, Lactobacillus delbrueckii, helveticus et Oenococcus oeni).These related to Eukaryotic oe-crystallins able form high complexes with a chaperoning activity.Induction factors vary bacterium.The expression L. and O. oeni genes governed by different promoter structures.In helveticus, regulation...