A5024402171- Electrochemical Analysis and Applications
- Analytical Chemistry and Chromatography
- Edible Oils Quality and Analysis
- Fermentation and Sensory Analysis
- Vanadium and Halogenation Chemistry
- Metal complexes synthesis and properties
- Analytical chemistry methods development
- Advanced Chemical Sensor Technologies
- Arsenic contamination and mitigation
- Phytochemicals and Antioxidant Activities
- Horticultural and Viticultural Research
- Heavy metals in environment
- Probiotics and Fermented Foods
- Essential Oils and Antimicrobial Activity
- Plant Surface Properties and Treatments
- Postharvest Quality and Shelf Life Management
- Chemical and Physical Properties in Aqueous Solutions
- Magnetism in coordination complexes
- Tea Polyphenols and Effects
- Coffee research and impacts
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Organometallic Compounds Synthesis and Characterization
- Water Quality Monitoring and Analysis
- Dye analysis and toxicity
Universidade Nova de Lisboa
2000-2014
Instituto de Biologia Experimental e Tecnológica
1996-2013
Instituto de Medicina Tropical
2012
Universidade de São Paulo
2012
Instituto de Tecnología Química
2010
Instituto Superior Técnico
1991-2008
Instituto Politécnico de Lisboa
1991-2008
Instituto Nacional de Investigação Agrária e Veterinária
2000
Cardiff University
1991
Rede de Química e Tecnologia
1990
Extracts of leaves, fruits and seeds olive tree cultivars Trás-os-Montes e Alto Douro (Portugal) were analysed by reverse phase HPLC with diode array detection mass spectrometry (MS). This methodology allowed the identification some common phenolic compounds, namely, verbascoside, rutin, luteolin-7-glucoside, oleuropein hydroxytyrosol. Moreover hyphenation MS enabled nüzhenide in seeds. An glucoside was also detected leaves. The total content determined Folin Denis reagent antioxidant...
The purpose of this work was to screen for and characterize the potential probiotic features strains lactic acid bacteria isolated from Galega cultivar fermented olives, eventually develop an improved food plant origin. From 156 strains, 10 were – bile salt tolerant, exhibited survival rates up 48%, following simulated digestion. All auto- (4–12%) co-aggregation (≥30%), as well hydrophobicity (5–20%) exopolysaccharide-producing abilities, while no strain possessed haemolytic capacity or...
A mixed-valence complex of vanadium-(IV) and -(V). Na[V2O3(DL-salser)2]·5H2O (salser =N-salicylideneserinate) has been isolated from solutions containing oxovanadium(IV), DL-serine salicylaldehyde. Its structure determined by X-ray diffraction analysis: space group P21/c, a= 12.845(5), b= 16.615(4), c= 13.072(0)Å, β= 103.53(9)°, Z= 4 R= 0.0494 for 2860 observed reflections. The V2O33+ unit in this a V–O–V angle smaller than the usual values each ion contains D- L-serine residue. Each sodium...
The analysis of BTEX and other substituted benzenes in water samples using solid phase microextraction (SPME) quantification by gas chromatography with flame ionization detection (GC-FID) was validated. best analytical conditions were obtained PDMS/DVB/CAR fibre headspace extraction (HS-SPME) at 50 °C for 20 min without stirring. linear range each compound HS-SPME GC/FID defined. limits these compounds were: benzene (15 ng L−1), toluene (160 monochlorobenzene (54 ethylbenzene (32 m-xylene...
The seed extracts of olive tree cultivars were analyzed by reverse phase HPLC with diode array detection and mass spectrometry. hyphenation spectrometry (ESI source) in both polarity modes enabled the identification seeds nüzhenide 11-methyl oleoside, among other oleosides, means MSn. methods used allowed us to obtain profiles phenolic components conclude that they are mainly secoiridoids. quantification detected secoiridoids was also achieved using ultraviolet (λ = 240 nm) which comparison...
We investigated the clinical impact of human coronaviruses (HCoV) OC43, 229E, HKU1 and NL63 in pediatric patients with cystic fibrosis (CF) during routine exacerbation visits. A total 408 nasopharyngeal aspirate samples were obtained from 103 over a 1-year period. Samples positive for HCoV submitted nucleotide sequencing to determine species. Nineteen (4.65%) HCoV, which 8 NL63, 6 4 HKU1, 1 229E. Identification was not associated an increased rate respiratory exacerbations, but NL63-positive...
BACKGROUND Plants have developed an efficient system of recognition that induces a complex network signalling molecules such as salicylic acid (SA), jasmonic (JA) and abscisic (ABA) in case pathogenic infection. The use specific sensitive methods is mandatory for the analysis compounds these samples. RESULTS In this study liquid chromatography/electrospray ionisation tandem mass spectrometry method was validated simultaneous quantification SA, JA ABA Coffea arabica (L.) leaves order to...
Gall-inducing insects display intimate interactions with their host plants, usually described as parasitic relationships; the galls seem to favor galler alone. We report on a case in which presence of induced by Leptocybe invasa Fisher & LaSalle (Hymenoptera; Eulophidae) benefit its plant, river red gum Eucalyptus camaldulensis Dehnh. Field observations showed that E. plants infected this gall wasp were less susceptible cold injury than neighboring conspecific without galls. In laboratory,...
The enzymatic production of ethyl butyrate was studied: the lipase Candida rugosa (E.C. 3.1.1.3.) immobilized in a polyurethane matrix and subsequently introduced an organic medium containing substrates appropriate concentrations. large majority experiments carried out n-hexane. Two further solvents were tested, namely n-heptane n-dodecane. partition coefficients matrix/solvent estimated for various solvent systems. initial esterification rate, molar yield ester/acid degree conversion found...
The vanadium(IV) octaco-ordinated complex (NH4+)[N(CH3)4+][V(HIDA)2](HIDA =N-hydroxy-iminodiacetate), whose anion is a likely model for the natural compound 'amavadin,' has been prepared and its crystal molecular structures have determined by X-ray crystallography; this first example of an with just N O donor atoms.
‘Galega' is the main Portuguese olive variety providing greatest percentage of table production from homemade and industrial methods. In this work a better understanding fermentation involved in both methods intended. Yeasts lactic acid bacteria (LAB) constitute microflora acting fermentation, with Pichia membranaefaciens being dominant yeast specie present throughout process. LAB develop their activity mainly along second stage where Lactobacillus plantarum pentosus were isolated...