- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Insect and Pesticide Research
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Agronomic Practices and Intercropping Systems
- Cocoa and Sweet Potato Agronomy
- Plant Pathogens and Fungal Diseases
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Agricultural pest management studies
- Plant pathogens and resistance mechanisms
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Phytoplasmas and Hemiptera pathogens
- Botanical Research and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Plant and animal studies
- Peanut Plant Research Studies
Universidade Metodista de São Paulo
2022-2025
Universidade de São Paulo
2022-2025
Universidade Brasil
2023-2025
Universidade Estadual Paulista (Unesp)
2017-2024
Agricultural Research Organization
2020-2021
Dead Sea and Arava Science Center
2020-2021
Proteca (Brazil)
2019
The Bemisia tabaci is a polyphagous insect and successful vector of plant viruses. B. species complex in Brazil native from the New World (NW) group, as well invasive species, Middle East-Asia Minor 1 (MEAM1) Mediterranean (MED) were reported. For better understanding distribution different four years after invasion Brazil, whiteflies collected 237 locations throughout country between 2013 2017, identified facultative endosymbionts detected. survey revealed that MEAM1 was prevalent found on...
Abstract Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify virus isolate present in symptomatic S. caterpillars. sample extracted and OBs purification performed. Viral DNA using the PureLink RNA/DNA Mini Kit (ThermoFisher) primers prl8-1 prl8-1B were used for amplification gene fragment. identified showing high genetic similarity with “Spodoptera nucleopolyhedrovirus CNPSo-165”, characterizing itself novelty scenario...
ABSTRACT In 2023, Adenium obesum plants displaying virus‐like symptoms were observed in two locations Brazil. High‐throughput sequencing (HTS) analysis of RNA‐Seq one sample yielded a contig sequence 3923 nucleotides (nt), showing 97.77% nt identity with the genome pelargonium flower break virus (PFBV, genus Alphacarmovirus ). Total RNA was also extracted from another sample, and presence PFBV confirmed by real‐time reverse transcription‐polymerase chain reaction (RT‐qPCR) these samples....
ABSTRACT In 2024, a tobamovirus was found in symptomless prickly pear ( Opuntia leucotricha ) plant Brazil. Transmission electron microscopy examinations revealed tobamovirus‐like particles ca. 300 nm long negatively stained cladode's extracts from an asymptomatic O. plant. High‐throughput sequencing resulted 6374 nucleotide contig that shares 98.68% sequence identity with rattail cactus necrosis‐associated virus (RCNaV, Tobamovirus muricaudae ), first detected Aporocactus flagelliform,...
Pereskia aculeata Miller is a climbing cactus, native to tropical areas of the Americas, whose leaves are used in cooking and folk medicine. Known as “Barbados gooseberry”, “leaf cactus” “ora-pro-nóbis” (OPN), it considered an unconventional food plant, valued for its high protein mineral content, offering sustainable healthy options. In 2024, three OPN plants displaying ringspots, mosaic leaf distortion were collected vegetable garden at Instituto Biológico, São Paulo, Brazil. Elongated...
ABSTRACT An isolate of the tobamovirus tobacco mild green mosaic virus (TMGMV), Tobamovirus mititessellati, was found naturally infecting petunia (Petunia × hybrida) cv. Pinstripe, causing reduced leaf size, mottling, and color breaking flowers, in an experimental field at Piracicaba, São Paulo state, Brazil. TMGMV identified by virion morphology, cytopathology, transmission experiments, molecular assays. The entire nucleotide sequence this (TMGMV-Pet) obtained demonstrated, essence, to be...
Abstract The whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most important agricultural pests and virus vectors worldwide. considered a complex cryptic species with at least 44 species. Among them, Middle East-Asia Minor 1 (MEAM1, formerly B biotype) Mediterranean (MED, Q are important, they have attained global status. In Brazil, MEAM1 was first reported in 1990s currently predominant country, meanwhile, MED recently South Southeast regions found to be mainly...
Abstract Since the detection of Bemisia tabaci Middle East‐Asia Minor 1 (MEAM1) species in Brazil early 1990s, this whitefly has been predominant and main viral vector country. In work, whiteflies were collected from commercial soybean fields near far greenhouses where Mediterranean (MED) had previously detected infesting vegetable crops. Results indicated that MEAM1 was most sampling sites, while MED found colonizing plants open field conditions alone and/or with several places. Among...
Abstract In 2022, virus‐like symptoms were observed in Adenium obesum plants Brazil. High‐throughput sequencing analysis found three contig sequences of cucumber mosaic virus (CMV) genome segments, which 3352, 3006 and 2221 nucleotides, shared 98.59%, 95.02% 98.05% nucleotide identities with the CMV RNAs 1, 2 3, respectively. Phylogenetic coat protein gene indicated that isolate belongs to subgroup IB. Symptoms mottle like those field samples reproduced on mechanically inoculated A. obesum,...
Abstract Begomoviruses cause losses in several crops around the world. Whiteflies of Bemisia tabaci cryptic species MEAM1 and MED are efficient vectors begomoviruses. has been predominant Brazil since mid‐1990s. was detected 2014, then, high insect infestations have reported, mainly greenhouse production solanaceous crops. In this study, we compared transmission efficiency begomoviruses tomato severe rugose virus (ToSRV) mosaic (ToRMV) to ( Solanum lycopersicum ) bean golden (BGMV) common...
Abstract Whiteflies are important agricultural pests of several crops and cause great economic losses, mainly by the transmission plant viruses. Among different species whiteflies, Trialeurodes vaporariorum Bemisia tabaci MEAM 1 (B biotype) transmit crinivirus Tomato chlorosis virus (To CV ). Previous studies report that virus‐infected plants can influence attractiveness behaviour whiteflies. In this study, we evaluated number eggs, egg hatching rate emergence adults both T. B. on To...
Soybean stem necrosis is caused by cowpea mild mottle virus (CPMMV), transmitted the whitefly Bemisia tabaci . CPMMV has already been recorded in all major soybean-producing areas of Brazil. The impacts to current Brazilian soybean production are unknown, thus main objective this study was evaluate effects infection on important cultivars grown Southern and Midwestern regions Although asymptomatic some tested cultivars, significantly reduced plant height, number pods per 1,000-grain weight....
Abstract Tomato severe rugose virus (ToSRV) is the most important begomovirus transmitted and spread by whitefly Bemisia tabaci in tomato crops Brazil. Cultural practices are being adopted, along with insecticides, for controlling this virus. However, little known about importance of weeds pathosystem, which can contribute to failure these practices. This work aimed evaluate role Datura stramonium Nicandra physaloides as alternative hosts ToSRV verify viral influence on biological...
Abstract In 2023, virus‐like symptoms were observed in Pelargonium zonale plants Brazil. Transmission electron microscope analysis revealed the presence of isometric particles measuring ca. 30 nm diameter leaf extracts and ultrathin sections symptomatic field plants. Molecular assays using PFBV‐specific primers sequencing amplicons identified pelargonium flower break virus (PFBV, genus Alphacarmovirus ). Mechanically inoculated Chenopodium quinoa P. exhibited chlorotic local lesions systemic...
Cowpea mild mottle virus (CPMMV, genus Carlavirus, family Betaflexividae) is an economically important infecting soybeans in Brazil, where it was initially identified 1983. CPMMV transmitted by the whitefly, Bemisia tabaci, and occasionally seeds. Over last three decades, most invasive B. tabaci Middle East–Asia Minor 1 (MEAM1), lately Mediterranean (MED) cryptic species, have replaced indigenous species with MEAM1 being predominant. In this study, we investigated transmission properties of...
The silverleaf whitefly, Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae), is a significant agricultural pest worldwide, impacting variety of crop yields. Since the introduction B. Mediterranean (MED) species in Brazil, limited research has measured relative efficacy primary insecticides used whitefly management. This study evaluated susceptibility three distinct MED populations to 11 insecticide active ingredients and characterized bacterial endosymbionts within each population. tested...
HomePlant DiseaseVol. 104, No. 2First Report of Tomato Chlorosis Virus Infecting Cucumber in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst BrazilV. H. Bello, E. S. Gorayeb, L. F. M. Watanabe, B. R. De Marchi, Ribeiro-Junior, Vicentin, da Silva, and Krause-SakateV. Bello†Corresponding author: V. Bello; E-mail Address: vhbello@hotmail.comhttp://orcid.org/0000-0002-2869-8119Faculdade de Ciências Agronômicas, UNESP, Botucatu, SP, Brazil,...
Pepper cultivation in Israel has been constrained by two sympatric poleroviruses, vein yellows virus-2 (PeVYV-2) and whitefly-borne virus (PeWBVYV), which are transmitted specifically aphids whiteflies, respectively. This study investigated the interaction between PeVYV-2 PeWBVYV inside host plant insect vectors. Our results show that compete against each other also aphids. was weaker competitor plant, with diminished transmission rates when inoculated simultaneously or successively after...
Sunflower (Helianthus annuus) plants showing symptoms of chlorosis, mosaic, chlorotic ringspot, and necrosis on younger leaves were found in a small experimental plot Piracicaba, the state São Paulo, Brazil. Preliminary examinations by transmission electron microscopy symptomatic leaf tissue revealed flexuous filamentous particles 13-15 nm wide 700-750 long, cytoplasmatic cylindrical inclusions typical those plant cells infected members Potyvirus genus. Total RNA extracted from subjected to...