A5082983821- Glioma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Pluripotent Stem Cells Research
- Cancer Genomics and Diagnostics
- Neurogenesis and neuroplasticity mechanisms
- Chromatin Remodeling and Cancer
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- RNA Research and Splicing
- Renal and related cancers
- SARS-CoV-2 and COVID-19 Research
- Telomeres, Telomerase, and Senescence
- CRISPR and Genetic Engineering
- Microtubule and mitosis dynamics
- Hedgehog Signaling Pathway Studies
- Protein Degradation and Inhibitors
- Meningioma and schwannoma management
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Head and Neck Cancer Studies
- Endoplasmic Reticulum Stress and Disease
- Cancer therapeutics and mechanisms
Medical University of Lodz
2016-2025
Pro Persona
2021-2022
Biolog (United States)
2020
CS Diagnostics
2019
Temple University
2005-2008
In-Q-Tel
2005
Memorial Hospital of South Bend
2004
Drexel University
2001
It has recently been reported by several sources that original (i.e., present in vivo) glioma cell phenotypes or genotypes cannot be maintained vitro. For example, glioblastoma lines presenting EGFR amplification established. IDH1 sequencing and loss of heterozygosity analysis was performed for 15 surgery samples astrocytoma early late passages cells derived from those 11 archival samples. We were not able to culture tumour mutations originating currently proceeded 10 tumours; the same...
Abstract Introduction The prospect of therapeutic applications the induced pluripotent stem cells (iPSCs) is based on their ability to generate virtually any cell type present in human body. Generation iPSCs from somatic has opened up new possibilities investigate biology, better understand pathophysiology diseases, and design therapy approaches field regenerative medicine. In this study, we focus episomal system, a non-viral integration-free reprogramming method derive various origin....
Abstract Glial fibrillary acidic protein (GFAP)‐positive cells derived from the neurogenic areas of brain can be stem/progenitor and give rise to new neurons in vitro vivo . We report here that a population GFAP‐positive fetal human parenchyma coexpress markers early neural neuronal cells, have progenitor cell characteristics. used monolayer culture system expend differentiate these cells. During initial proliferative phase, all expressed GFAP, nestin low levels βIII‐tubulin. When were...
Induced pluripotent stem cells (iPSC) possess an enormous potential as both, scientific and therapeutic tools. Their application in the regenerative medicine provides new treatment opportunities for numerous diseases, including type 1 diabetes. In this work we aimed to derive insulin producing (IPC) from iPS established defined conditions. We optimized iPSC generation protocol created cell lines with stably integrated PDX1 NKX6.1 transgenes under transcriptional control of...
Although features of variable differentiation in glioblastoma cell cultures have been reported, a comparative analysis properties normal neural GFAP positive progenitors, and those shown by cells, has not performed.Following methods were used to compare cells GFAP+NNP (NHA): exposure medium, adipogenic osteogenic western blot analysis, immunocytochemistry, single assay, BrdU incorporation assay. To characterize EGFR amplification LOH/MSI P53 nucleotide sequence performed.In vitro cancer...
The Epidermal Growth Factor Receptor (EGFR) and its mutations contribute in various ways to tumorigenesis biology of human cancers. They are associated with tumor proliferation, progression, drug resistance the process apoptosis. There also reports that overexpression activation wild-type EGFR may lead cell To study this phenomenon, we overexpressed an AD293 line two most frequently observed forms receptor: constitutively active mutant-EGFR variant III (EGFRvIII). Then, compared effect EGF...
Glioblastoma cell cultures in vitro are frequently used for investigations on the biology of tumors or new therapeutic approaches. Recent reports have emphasized importance culture type maintenance tumor original features. Nevertheless, ability GBM cells to preserve EGFR overdosage remains controversial. Our experimental approach was based quantitative analysis gene dosage both at DNA and mRNA level. Real-time PCR data were verified with a FISH method allowing distinction between...
Glioblastoma is a highly aggressive tumour of the central nervous system, characterised by poor prognosis irrespective applied treatment. The aim our study was to analyse whether molecular markers glioblastoma (i.e. TP53 and IDH1 mutations, CDKN2A deletion, EGFR amplification, chromosome 7 polysomy EGFRvIII expression) could be associated with distinct and/or response therapy. Moreover, we describe method which allows for reliable, as well time- cost-effective, screening amplification...
Primary cancer cells constitute a favourable testing platform for in vitro research oncology field as they reflect tumour state more accurately than the most commonly employed stable cell lines. Unfortunately, due to limited availability of material and difficulties with protocols validation, primary models are rarely implemented into laboratory practice. We have compared cultures, differing media components plate coatings. In terms culture establishment, application Geltrex® coating...
Small-molecule compounds that even partially restore the GTPase activity of RASG12V can be used in anticancer therapy. Until now, attempts to obtain such have failed. Compounds with this ability been defined our research. The were initially identified through virtual screening, and their optimal binding conformation RAS SW-II pocket was determined using flexible docking technique. Efficacy verified based on IC50 determination, activity, as well AKT ERK phospho WB assays. tested significantly...
Glioblastoma is the most common and malignant brain tumor, characterized by high cellular heterogeneity. About 50% of glioblastomas are positive for EGFR amplification, half which express accompanying mutation, encoding truncated constitutively active receptor termed EGFRvIII. Currently, no cell models suitable development EGFRvIII-targeting drugs exist, while available ones lack intratumoral heterogeneity or extrachromosomal nature EGFRvIII.The reports regarding biology EGFRvIII expressed...
Cell line analysis is an important element of cancer research. Despite the progress in glioblastoma cell culturing, cells isolated from majority specimens cannot be propagated infinitely vitro. The aim this study was to identify processes responsible for stabilization failure. Therefore, we analyzed 56 primary GB cultures, 7 which were stabilized. Our results indicate that senescence primarily failure, while mitotic catastrophe and apoptosis play a minor role. Moreover, new technical...
Recent research results have confirmed the high significance of OPG/RANK/RANKL system in development bone diseases. The aim reported study was to assess gene expression levels peripheral blood mononuclear cells (PBMCs) after strontium ranelate (SR) and ibandronate administered patients with postmenopausal osteoporosis. A total 89 women, aged 51 85 years, Outpatient Clinic Osteoporosis Military Teaching Hospital Lodz, were enrolled into study. randomly assigned different medical therapies:...
Background The high frequency of mutations in the isocitrate dehydrogenase 1 (IDH1) gene diffuse gliomas indicates its importance process gliomagenesis. These result loss normal function and acquisition neomorphic activity converting α-ketoglutarate to 2-hydroxyglutarate. This potential oncometabolite may induce epigenetic changes, resulting deregulated expression numerous genes, including those related differentiation or cell survivability. Methods Neural stem cells were derived from human...
Cell therapy offers hope, but it also presents challenges, most particularly the limited ability of human organs and tissues to regenerate. Since many diseases are associated with irreversible pathophysiological or traumatic changes, stem cells their derivatives unable secure healing. Although regenerative medicine chances for improvements in diseases, such as type one diabetes Parkinson's disease, cannot eliminate primary cause them. While successes can be expected sickle cell this is not...
Tumorigenic potential of induced pluripotent stem cells (iPSCs) infiltrating population neural (iNSCs) generated from iPSCs may limit their medical applications. To overcome such a difficulty, direct reprogramming adult somatic into iNSCs was proposed. The aim this study the systematic comparison (iNc) obtained with different methods—direct human fibroblasts either SOX2 (SiNSc-like) or and c-MYC (SMiNSc-like) differentiation to ebiNSc—in terms gene expression profile, as well proliferation...