Abstract DNA has been used as a scaffold to stabilize small, atomically monodisperse silver nanoclusters, which have attracted attention due their intriguing photophysical properties. Herein, we describe the X‐ray crystal structure of DNA‐encapsulated, near‐infrared emitting Ag 16 nanocluster (DNA–Ag NC). The asymmetric unit contains two DNA–Ag NCs and packing between is promoted by several interactions, such silver‐mediated base pairs 3′‐terminal adenines, phosphate–Ca 2+ –phosphate...

DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method linking well-characterized DNA-AgNC molecules interest is presented. Three different peptides small protein, human insulin, were tested as labeling targets. The target compounds was verified...

Due to desirable optical properties, such as efficient luminescence and large Stokes shift, DNA-templated silver nanoclusters (DNA-AgNCs) have received significant attention over the past decade. Nevertheless, excited-state dynamics of these systems are poorly understood, studies processes ultimately leading a fluorescent state scarce. Here we investigate early time relaxation 16-atom cluster (DNA-Ag16NC) featuring NIR emission in combination with an unusually shift 5000 cm–1. We follow...

Abstract DNA‐stabilized silver nanoclusters (DNA‐AgNCs) are a class of emitters that have primarily been studied for their molecular‐like photophysical properties with ns‐lived fluorescence. However, µs‐lived luminescence has recently reported an increasing number DNA‐AgNCs, but little is still known about the origin long‐lived emission. To deepen understanding state, (DNA) 2 ‐[Ag 11 ] 7+ , emitter short‐ and dual emission, studied. By examining crystals it found fluorescence transitions...

Silver nanoclusters stabilized by 5′-CCCGGAGAAG-3′ DNA strands display an unusually high fluorescence quantum yield in the near-infrared region.

The near-infrared (NIR) I and II regions are known for having good light transparency of tissue less scatter compared to the visible region electromagnetic spectrum. However, number bright fluorophores in these is limited. Here we present a detailed spectroscopic characterization DNA-stabilized silver nanocluster (DNA-AgNC) that emits at around 960 nm solution. DNA-AgNC converts blue-shifted emitters over time. Embedding DNA-AgNCs poly(vinyl alcohol) (PVA) shows they photostable enough be...

DNA oligomers are known to serve as stabilizing ligands for silver nanoclusters (AgN-DNAs) with rod-like nanocluster geometries and nanosecond-lived fluorescence. Here, we report two AgN-DNAs that possess distinctly different structural properties the first exhibit only microsecond-lived luminescence. These emitters characterized by significant broadband downconversion from ultraviolet/visible near-infrared region. Circular dichroism spectroscopy shows structures of these differ...

Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic hemodynamic studies but challenging due to complexity sensing individual particles within circulatory system a live animal. Here, we demonstrate that DNA-stabilized silver nanocluster (DNA-Ag16NC) emits first near-infrared window upon two-photon excitation second NIR can be used multiphoton vivo fluorescence correlation spectroscopy measurement cerebral blood flow rates mice with...

DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters formed by atoms and cations encapsulated in DNA oligomers. Here, we present an analytical approach to calculate the molar absorption coefficient (ε) of these systems, which consists combining UV-Vis spectroscopy, electrospray ionization-mass spectrometry (ESI-MS), inductively coupled plasma-optical emission (ICP-OES). ESI-MS enables determination number silvers bound strands, whereas ICP-OES allows measurement total...

In this paper we present a new near-IR emitting silver nanocluster (NIR-DNA-AgNC) with an unusually large Stokes shift between absorption and emission maximum (211 nm or 5600 cm-1). We studied the effect of viscosity temperature on steady state time-resolved emission. The results NIR-DNA-AgNC show that relaxation dynamics slow down significantly increasing solvent. high solution, spectral stretches well into nanosecond scale. As result in solutions, multi-exponential fluorescence decay time...

The nanosecond excited state temporal and spectral relaxation of a purified, red-emitting DNA-templated silver nanocluster (DNA-AgNC) was characterized as function temperature. findings are explained by introducing phenomenological electronic structure diagram. reproducibility cyclability the average decay time opens up possibility using DNA-AgNCs for time-based nanothermometry.

DNA-stabilized silver nanoclusters (DNA-AgNCs) are easily tunable emitters with intriguing photophysical properties. Here, a DNA-AgNC dual emission in the red and near-infrared (NIR) regions is presented. Mass spectrometry data showed that two DNA strands stabilize 18 atoms nanocluster charge of 12+. Besides determining composition DNA2 [Ag18 ]12+ , steady-state time-resolved methods were applied to characterize picosecond fluorescence relatively intense microsecond-lived NIR luminescence....

Abstract The combination of mass spectrometry and single crystal X‐ray diffraction HPLC‐purified DNA‐stabilized silver nanoclusters (DNA‐AgNCs) is a powerful tool to determine the charge structure encapsulated AgNC. Such information not only relevant design new DNA‐AgNCs with tailored properties, but it also important for bio‐conjugation experiments essential electronic calculations. Here, efforts green emissive DNA‐AgNC are presented. Unfortunately, original DNA‐AgNC, known have four...

Abstract DNA‐stabilized silver nanoclusters (DNA‐Ag N ) are atomically precise and sequence‐tuned nanomaterials with potential applications for deep tissue biomedical imaging. Here, a dual‐emissive DNA‐Ag is presented fluorescence in the first near‐infrared (NIR‐I) spectral window microsecond‐lived photoluminescence second (NIR‐II) window. High‐resolution electrospray ionization mass spectrometry showed that emitter has molecular formula (DNA) 2 [Ag 17 ] 11+ . The crystallization of was...

We determine the first solution‐state structure of a DNA‐stabilised Ag16Cl2 nanocluster using X‑ray total scattering and pair distribution function analysis. find that in solution exhibits both displacive rotational distortions compared to known crystal structure. Additionally, our measurements are sensitive towards changes DNA conformation, revealing scaffold significantly more flexibility than when conformationally locked crystalline form. Our results demonstrate capability clusters beyond...

We determine the first solution‐state structure of a DNA‐stabilised Ag16Cl2 nanocluster using X‑ray total scattering and pair distribution function analysis. find that in solution exhibits both displacive rotational distortions compared to known crystal structure. Additionally, our measurements are sensitive towards changes DNA conformation, revealing scaffold significantly more flexibility than when conformationally locked crystalline form. Our results demonstrate capability clusters beyond...

A single-stranded DNA-based (ssDNA) dyad was constructed comprising 15 silver atoms stabilized by a ssDNA scaffold (DNA-AgNC) and an Alexa 546 fluorophore bound to the 5′ end. The chosen function as Förster resonance energy transfer (FRET) donor for AgNC. Time-correlated single photon counting (TCSPC) experiments allowed unraveling excited-state relaxation processes of purified DNA-AgNC-only system. TCSPC results revealed slow dynamics red shift emission spectrum during lifetime. from model...

We investigated an ss-DNA sequence that can stabilize a red- and green-emissive silver nanocluster (DNA-AgNC). These two emitters convert between each other in reversible way. The change from to green-emitting DNA-AgNCs be triggered by the addition of H2O2, while opposite conversion achieved NaBH4. Besides demonstrating switching determining recoverability, we fully characterized photophysical properties, such as steady-state emission, quantum yield, fluorescence lifetime, anisotropy...

Photophysical characterization of two DNA-AgNCs emitting in the 800–900 nm range and observation microsecond luminescence.

We investigated the effect of using D 2 O versus H as solvent on spectroscopic properties two NIR emissive DNA-stabilized silver nanoclusters (DNA–AgNCs).

A method for measuring emission over a range of sub-nanosecond to millisecond timescales is presented and demonstrated DNA-stabilized silver nanocluster (DNA-AgNC) displaying dual emission.

DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties. They capable generating anti-Stokes fluorescence upon excitation near-infrared lasers. The has previously been speculated to be either the result consecutive photon absorption (upconversion) or hot band (HBA). Here, we revisit DNA-AgNCs determine underlying mechanistic origin. We investigate two studied together organic and emission features in same spectral regions as...

Fluorescence imaging is a key tool in biological and medical sciences. Despite the potential for increased depth near‐infrared range, limited availability of bright emitters hinders its widespread implementation. In this work, DNA‐stabilized silver nanocluster (DNA–AgNC) with emission at 960 nm solution presented, which redshifts further to 1055 solid crystalline states. The atomic structure, composition charge DNA–AgNC are determined by combining single‐crystal X‐ray diffraction...

Optically activated delayed fluorescence and upconversion allow removing unwanted auto-fluorescence.