A5076743849- Regulation of Appetite and Obesity
- Reproductive biology and impacts on aquatic species
- Hypothalamic control of reproductive hormones
- Aquaculture Nutrition and Growth
- Physiological and biochemical adaptations
- Biochemical Analysis and Sensing Techniques
- Aquaculture disease management and microbiota
- Neuropeptides and Animal Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Zebrafish Biomedical Research Applications
- melanin and skin pigmentation
- Neurobiology and Insect Physiology Research
- Cephalopods and Marine Biology
- Neuroendocrine regulation and behavior
- Sleep and Wakefulness Research
- Fish Ecology and Management Studies
- Animal Behavior and Reproduction
- Ovarian function and disorders
- Synthesis and Characterization of Heterocyclic Compounds
- Animal Genetics and Reproduction
- Stress Responses and Cortisol
- Adipose Tissue and Metabolism
- Ecology and Conservation Studies
- Geology and Paleoclimatology Research
- Marine Toxins and Detection Methods
Kitasato University
2014-2024
University of Toyama
2009
Japan Society for the Promotion of Science
2009
The pars tuberalis of the pituitary gland is regulatory hub for seasonal reproduction in birds and mammals. Although fish also exhibit robust responses, they do not possess an anatomically distinct tuberalis. Here we report that saccus vasculosus a sensor. We observe expression key genes regulating rhodopsin family masu salmon. Immunohistochemical studies demonstrate all these are expressed coronet cells vasculosus, suggesting existence photoperiodic signalling pathway from light input to...
We recently identified a cDNA encoding three novel fish hypothalamic neuropeptides, having LPXRF-NH 2 from the goldfish brain. In this study, to clarify physiological functions of these LPXRFamide peptides (gfLPXRFa-1, -2, and -3), we analysed localisation hypophysiotrophic activity using sockeye salmon, Oncorhynchus nerka , in which immunoassay systems for several anterior pituitary hormones have been developed. gfLPXRFa-immunoreactive cell bodies were detected nucleus posterioris...
In diurnal and nocturnal organisms, daily activity is regulated by the perception of environmental stimuli circadian rhythms, which enable organisms to maintain their essential behaviors. The Japanese sand lances genus Ammodytes are coastal marine fish that exhibit unique burrowing behavior. To elucidate extrinsic intrinsic regulation this behavior its endocrinological basis, we conducted a series rearing experiments under various light conditions hormone administrations. Under light-dark...
We examined whether gonadotropin-releasing hormone (GnRH)-like peptides are present in the neural ganglia of gastropod Pacific abalone (Haliotis discus hannai) by reverse-phase high performance liquid chromatography (rpHPLC) combined with time-resolved fluoroimmunoassay (TR-FIA) analysis and immunohistochemistry. Cerebral ganglion extracts showed a similar retention time to lamprey GnRH-II (lGnRH-II) rpHPLC TR-FIA analysis. GnRH-like-immunoreactive (ir) cell bodies (which reacted mouse...
Orexins (orexin-A and -B) are involved in the regulation of food intake mammals. In barfin flounder, Verasper moseri, we previously reported that orexin-A-like-immunoreactive (ir) cell bodies localized hypothalamus, which is a possible orexigenic center fish. However, physiological roles orexin flounder remain unclear. Here, cloned prepro-orexin cDNA examined effects feeding status on gene expression to obtain better insight into orexins regulation. A molecular cloning study showed encodes...
We examined whether a gonadotropin-releasing hormone (GnRH)-like peptide exists in the central nervous system (CNS) of kuruma prawn, Marsupenaeus japonicus, by reverse-phase high performance liquid chromatography (rpHPLC) combined with time-resolved fluoroimmunoassay (TR-FIA) analysis and immunohistochemistry. The displacement curve obtained for serially diluted extracts prawn brain paralleled chicken GnRH-II (cGnRH-II) standard cGnRH-II TR-FIA using anti-cGnRH-II antibody, which...
Dogfish (Squalus acanthias) growth hormone (GH) was identified by cDNA cloning and protein purification from the pituitary gland. GH encoded a prehormone of 210 amino acids (aa). Sequence analysis purified revealed that is composed signal peptide 27 aa mature 183 aa. showed 94% sequence identity with blue shark GH, also 37-66%, 26%, 48-67% osteichtyes, an agnathan, tetrapods. The site production through immunocytochemistry to be cells proximal pars distalis stimulates both insulin-like...
Neuropeptides with the Arg-Phe-amide motif at their C termini (RFamide peptides) were identified in brains of several vertebrates, and shown to have important physiological roles neuroendocrine, behavioral, sensory, autonomic functions. The present study RFamide peptides, which are teleost prolactin-releasing peptide (PrRP) homologs, sea lamprey, Petromyzon marinus characterized effect on release pituitary hormones vitro. Two peptides (RFa-A RFa-B) isolated from an acid extract lamprey...