A5049921302- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Magnetic and transport properties of perovskites and related materials
- Hepatitis B Virus Studies
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- Analytical Chemistry and Sensors
- HIV-related health complications and treatments
- Hepatitis C virus research
- Multiferroics and related materials
- Glycosylation and Glycoproteins Research
- Research in Social Sciences
- Viral Infections and Outbreaks Research
- Bacterial Infections and Vaccines
- Polyomavirus and related diseases
- Influenza Virus Research Studies
- Epilepsy research and treatment
- Alzheimer's disease research and treatments
- Malaria Research and Control
- Bipolar Disorder and Treatment
- Pneumonia and Respiratory Infections
University of Turku
2010-2024
International Centre for Genetic Engineering and Biotechnology
2010
Turku Centre for Biotechnology
2008
Abstract Multiplexed lateral flow assays (LFAs) offer efficient on-site testing by simultaneously detecting multiple biomarkers from a single sample, reducing costs. In cancer diagnostics, where can lack specificity, multiparameter detection provides more information at the point-of-care. Our research focuses on epithelial ovarian (EOC), STn-glycosylated forms of CA125 and CA15-3 antigens better discriminate benign conditions. We have developed dual-label LFA that detects both CA125-STn...
Abstract Detection of hepatitis B Virus surface antigen (HBsAg) is an established method for diagnosing both acute and chronic virus (HBV) infection. In addition to enzyme immunoassays (EIAs), rapid diagnostic tests (RDTs) are available the detection HBsAg in resource-poor settings. However, RDTs have inadequate sensitivity therefore not suitable diagnosis patients with low levels blood screening. To provide a high-sensitivity RDT, we developed lateral flow immunoassay (LFIA) utilizing...
Rapid diagnostic tests (RDTs) are often used for the detection of anti-human immunodeficiency virus (HIV) antibodies in remote locations low- and middle-income countries (LMIC) with low or limited access to central laboratories. The typical format an RDT is a lateral flow assay (LFA) visual interpretation prone subjectivity. This risk misinterpretation can be overcome luminescent upconverting nanoparticle reporters (UCNPs) measured miniaturized easy-to-use reader instrument. An LFA UCNPs...
The development of sensitive point-of-care (POC) assay platforms is interest for reducing the cost and time diagnostics. Lateral flow assays (LFAs) are gold standard POC systems, but their sensitivity as such inadequate, example, in case cardiac performance can be improved by incorporating different steps, pre-incubation to prolong interaction between sample reporter immunocomplex formation, washing steps background reduction. However, assays, manual conductor not desired. In this research,...
Myxovirus resistance protein A (MxA) is a biomarker of interferon-induced gene expression state involved in many viral infections and some autoimmune disorders. It has variety potential utilities clinical diagnostics, including distinguishing between bacterial infections. Currently, MxA-assays are used for monitoring IFN-β therapy multiple sclerosis (MS) patients. As proof-of-concept rapid quantitative measurement interferon response, lateral flow immunoassay (LFIA) with upconverting...
Plasmodium falciparum malaria is widespread in the tropical and subtropical regions of world. There ongoing effort to eliminate from endemic regions, sensitive point-of-care (POC) diagnostic tests are required support this effort. However, current POC not sufficiently detect P. asymptomatic individuals. After extensive optimization, we have developed a highly robust test for detection infection. The based on upconverting nanophosphor-based lateral flow (UCNP-LF) immunoassay. UCNP-LF was...
A novel recombinant multiepitope protein (MEP) has been designed that consists of four linear, immunodominant, and phylogenetically conserved epitopes, taken from human immunodeficiency virus (HIV)-encoded antigens are used in many third-generation immunoassay kits. This HIV-MEP evaluated for its diagnostic potential the detection anti-HIV antibodies sera. synthetic MEP gene encoding these joined by flexible peptide linkers a single open reading frame, was overexpressed Escherichia coli. The...
The Human Immunodeficiency Virus type 1 (HIV-1) envelope glycoprotein gp160, useful in detecting anti-HIV-1 antibodies, is difficult to express heterologous hosts. major hurdles are its signal sequence, strong hydrophobic regions and heavy glycosylation. While it has not been possible full length recombinant (r)-gp160 E. coli, can be expressed insect mammalian cells, but at relatively higher cost. In this work, we report coli-based over-expression of r-gp160 variant evaluate performance...
Abstract Background Several assays reliably measure cerebrospinal fluid (CSF) total tau (t‐tau) protein for Alzheimer’s diseases (AD) diagnosis. However, CSF sampling is invasive and hampers availability of biomarker testing in AD. Therefore, less blood t‐tau are needed, preferably on different platforms to enable implementation clinical settings. Point‐of‐care (POC) the development practical easily implemented assays. Here, we aim develop compare two POC‐assays, (1)on Ella microfluidics...
s: Critical and Point-of-Care Testing: Managing Technology for the Benefit of All Populations: 22nd International Symposium: September 17-20, 2008: Catalonia Barcelona Plaza Hotel|Barcelona, Spain