INTRODUCTION: Leptospirosis is a re-emerging zoonotic disease of humans and animals worldwide. The caused by pathogenic species the genus Leptospira. These organisms are maintained in nature via chronic renal infection carrier animals, which excrete their urine. Humans become infected through direct or indirect exposure to urine contact with contaminated water soil. This study was conducted investigate Leptospira infections as zoonosis that has been neglected Egypt. METHODS: Samples from...

Ringworm is a worldwide distributed contagious disease infecting both man and animals that constitute an economic, zoonotic, health problem concern all over the world. During last decade, attention has been directed to vaccination as ideal approach control of such diseases. In present study, non-adjuvanted polyvalent vaccines were prepared from locally isolated hot virulent dermatophyte species, namely Trichophyton verrucosum (T. verrucosum), mentagrophytes mentagrophytes), Microsporum canis...

Abstract Strangles is a highly contagious disease of the equine upper respiratory tract caused by Streptococcus equi subspecies. subsp. ( S. ) and zooepidemicus was isolated, as local, hot, field strains, from horses clinically suffering distress. The isolated Streptococci were identified using bacteriological molecular techniques. Four formulations inactivated vaccines developed evaluated. first formulation prepared isolates, adjuvanted with MONTANIDE GEL adjuvant, while second ISA-70...

The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera.A rapid (LFIT) has been developed, which SE Group D antigen labeled with the gold chloride molecules laid on conjugate pad. Staphylococcus aureus protein A was used as capture antibody at line (T) a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit were control (C) NC layout device.Using developed LFIT, minimal...

In the presented study, we developed a nanogold lateral glow immunoassay-based technique (LFI-COVID-19 antigen test) for detection of SARS-CoV-2 nucleocapsid proteins; LFI-COVID-19 Ag test has been tested limit (LOD), cross-reactivity and interfering substances, performance. It was found that performance when it evaluated by RT-qPCR indicated 95, 98, 97% sensitivity, specificity accuracy, respectively. This complies with WHO guidelines. concluded is point care an alternative approach to...

Rabies is a severe viral infection that causes acute encephalomyelitis and has case fatality rate of almost 100%. There no cure for symptomatic rabies, but post-exposure prophylaxis (PEP), which comprises vaccines anti-rabies immunoglobulins (RIGs), can successfully prevent the development irreversible clinical symptoms. In situation with low resources, primary PEP protocol confronts significant access implementation challenges could be overcome by using RIGs instead monoclonal antibodies...

mucous membranes and torn skin (De la Rua-Domenech, 2006).Bovine tuberculosis can affect a wide range of species is defined by the progressive development granulomatous lesions (tubercles/granulomas) in any body organ (Borham et al., 2022). The intradermal tuberculin test has been use for almost century diagnosis bovine Research

Summary. In this study 19 donkeys suffering from face excoriations below the medial canthas and lesions in lacrymal apparatus were subjected to clinical radiological microbiological investigations. Also histopathological examination of biopsy materials ocular made. Eleven out (57.9%) showed specific apparatus. Mycological revealed isolation Histoplasma cells 10 examined cases. The isolated culture, although not completely identical H. farciminosum , was more related than other species....

An affordable, sensitive, specific, fast, and easy to be delivered end-users immunochromatographic lateral flow kit (LFK) has been developed for the detection of β-lactams antibiotic residues in dairy milk.The LFK is based on a competitive format using anti-antibiotic-specific polyclonal antibodies.Specific artificially induced active acquired antibodies production were performed female New Zealand rabbits against following β-lactam antibiotics; Amoxicillin (AMX)-Keyhole Limpet Hemocyanin...

Summary: Two hundred horses in a horse farm with history of infedion ephtic lymphangitis were examined clinically, mycologically, serologically and by skin test using soluble antigen (‘Histofarch’) prepared from H. farciminosum. 3% the animals showed clinical manifestations disease 26.5% had antiiodies demonstrated Immuno D&sion (ID) Complement Fmtion Test (CFT). The ‘Histofarcin’ was applied to 40 representing negative positive cases, either apparently healthy, recovered previous...

Hybridomas that secreted antibodies against aflatoxin B1 for multiple uses were prepared using a unique immunization schedule. Aflatoxin B1-BSA conjugate was used of Balb/c mice. Spleen cells harvested from the hyper immunized mice to be fused with myeloma cell line (P3NS1) polyethylene glycol 3000, 50% concentration as fusogenic agent. The produced hybridomas selected HAT selective medium replaced by complete HT medium. From 10 th day after fusion, wells contain colonies covering 30% or...

Bovine tuberculosis is an infectious and chronic disease affecting cattle, caused by Mycobacterium bovis (M.bovis).The zoonotic nature of the has a serious worldwide impact on human health.Also, significant economic costs such in addition to deficiency precise estimates actual prevalence necessitate more efficient detection control measures, particularly developing countries.The main target present work was develop local, less expensive bovine interferon-gamma ELISA (Bo-IFN-γ ELISA) kit for...

Summary: 36 isolates of Microsporum gypseum recovered from Egyptian soil were found to be capable agglutinating red blood cells cow, buffalo, camel, sheep, chicken, duck, pigeon, Guinea pig and mouse. Also microconidial variants obtained dnring repeated subculturing had the same property. Haemagglutinins both wild forms partly thermolabile thermostable. They resisted effect trypsin, concentrated hydrochloric acid 4N sodium hydroxide, but they markedly inactivated by potassium periodate (wild...

The ultrastructure of cultured blood mondcyte-derived human macrophages was investigated and correlated under the effect different doses rh-GMCSF (dose 1=25IU/ml, dose 2 = 125IU/ml 3 250IU/ml). Resting showed irregular cell borders pseudopodia pushed out in all directions. Their cytoplasm depicted rough endoplasmic reticulum Golgi complex perinuclear area. Lipid globules, primary lysosomes mitochondria were characteristically prominent. rh-GMCSF-stimulated more voluminous their nuclei...

In the present work a lateral flow immunochromatographic test (LFT) for rapid detection of Clostri­dium perfringens toxins types, alpha (α), beta (β) and epsilon (ε) in clinical samples was developed. C. were prepared, purified inactivated with 0.2% formalin. Polyclonal antibodies specific to types α, β ε toxoids prepared rabbits guinea pigs. The toxoid polyclonal labelled gold chloride nanoparticles. toxin rabbit pigs goat anti-rabbit utilised development latter - evaluated samples....